scholarly journals Genetic environment and location of the lnu(A) and lnu(B) genes in methicillin-resistant Staphylococcus aureus and other staphylococci of animal and human origin

2012 ◽  
Vol 67 (12) ◽  
pp. 2804-2808 ◽  
Author(s):  
C. Lozano ◽  
C. Aspiroz ◽  
Y. Saenz ◽  
M. Ruiz-Garcia ◽  
G. Royo-Garcia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Human Origin ◽  
Methicillin Resistant ◽  
Genetic Environment

scholarly journals What Is the Origin of Livestock-Associated Methicillin-Resistant Staphylococcus aureus Clonal Complex 398 Isolates from Humans without Livestock Contact? An Epidemiological and Genetic Analysis

2015 ◽  
Vol 53 (6) ◽  
pp. 1836-1841 ◽  
Author(s):  
W. S. N. Lekkerkerk ◽  
W. J. B. van Wamel ◽  
S. V. Snijders ◽  
R. J. Willems ◽  
E. van Duijkeren ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clonal Complex ◽  
Unknown Origin ◽  
Human Origin ◽  
Methicillin Resistant ◽  
Content Type ◽  
Veal Calves ◽  
Indirect Transmission ◽  
Outbreak Isolate

Fifteen percent of all methicillin-resistantStaphylococcus aureus(MRSA) clonal complex 398 (CC398) human carriers detected in The Netherlands had not been in direct contact with pigs or veal calves. To ensure low MRSA prevalence, it is important to investigate the likely origin of this MRSA of unknown origin (MUO). Recently, it was shown that CC398 strains originating from humans and animals differ in the presence of specific mobile genetic elements (MGEs). We hypothesized that determining these specific MGEs in MUO isolates and comparing them with a set of CC398 isolates of various known origin might provide clues to their origin. MUO CC398 isolates were compared to MRSA CC398 isolates obtained from humans with known risk factors, a MRSA CC398 outbreak isolate, livestock associated (LA) MRSA CC398 isolates from pigs, horses, chickens, and veal calves, and five methicillin-susceptibleStaphylococcus aureus(MSSA) CC398 isolates of known human origin. All strains werespatyped, and the presence or absence of,scn,chp, φ3int, φ6int, φ7int,rep7,rep27, andcadDXwas determined by PCRs. The MRSA CC398 in humans, MUO, or MRSA of known origin (MKO) resembled MRSA CC398 as found in pigs and not MSSA CC398 as found in humans. The distinct human MSSA CC398spatype, t571, was not present among our MRSA CC398 strains; MRSA CC398 was tetracycline resistant and carried no φ3 bacteriophage withscnandchp. We showed by simple PCR means that human MUO CC398 carriers carried MRSA from livestock origin, suggestive of indirect transmission. Although the exact transmission route remains unknown, direct human-to-human transmission remains a possibility as well.

Silviavirus phage ɸMR003 displays a broad host range against methicillin-resistant Staphylococcus aureus of human origin

2019 ◽  
Vol 103 (18) ◽  
pp. 7751-7765 ◽  
Author(s):  
Chanthol Peng ◽  
Tomoko Hanawa ◽  
Aa Haeruman Azam ◽  
Cierra LeBlanc ◽  
Porsry Ung ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Host Range ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Broad Host Range ◽  
Human Origin ◽  
Methicillin Resistant

scholarly journals Phages for methicillin-resistant Staphylococcus aureus: an international trial

1999 ◽  
Vol 122 (2) ◽  
pp. 227-233 ◽  
Author(s):  
J. F. RICHARDSON ◽  
V. T. ROSDAHL ◽  
W. J. van LEEUWEN ◽  
A. M. VICKERY ◽  
A. VINDEL ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Human Origin ◽  
Methicillin Resistant ◽  
International Trial ◽  
Resistant Strains

An internationally agreed and validated set of phages is used worldwide for the typing of strains of Staphylococcus aureus of human origin. However, because of the sometimes reduced susceptibility of methicillin-resistant strains (MRSA) to these phages, some of the national typing centres use locally isolated and characterized sets of experimental phages. In this trial, 42 such phages were distributed to 6 centres and tested against 744 isolates of MRSA with the intention of defining a phage set to augment the international set. The use of these experimental phages increased the percentage typability from 75% with the international set to 93% and the number of identifiable lytic patterns from 192 to 424. A subset of 10 experimental phages was selected. When this subset was compared with the experimental panel, the typability rate was 91% and 370 distinct patterns were obtained. This subset of phages has been distributed for international trial.

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