Silviavirus phage ɸMR003 displays a broad host range against methicillin-resistant Staphylococcus aureus of human origin

2019 ◽  
Vol 103 (18) ◽  
pp. 7751-7765 ◽  
Author(s):  
Chanthol Peng ◽  
Tomoko Hanawa ◽  
Aa Haeruman Azam ◽  
Cierra LeBlanc ◽  
Porsry Ung ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Host Range ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Broad Host Range ◽  
Human Origin ◽  
Methicillin Resistant

scholarly journals What Is the Origin of Livestock-Associated Methicillin-Resistant Staphylococcus aureus Clonal Complex 398 Isolates from Humans without Livestock Contact? An Epidemiological and Genetic Analysis

2015 ◽  
Vol 53 (6) ◽  
pp. 1836-1841 ◽  
Author(s):  
W. S. N. Lekkerkerk ◽  
W. J. B. van Wamel ◽  
S. V. Snijders ◽  
R. J. Willems ◽  
E. van Duijkeren ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clonal Complex ◽  
Unknown Origin ◽  
Human Origin ◽  
Methicillin Resistant ◽  
Content Type ◽  
Veal Calves ◽  
Indirect Transmission ◽  
Outbreak Isolate

Fifteen percent of all methicillin-resistantStaphylococcus aureus(MRSA) clonal complex 398 (CC398) human carriers detected in The Netherlands had not been in direct contact with pigs or veal calves. To ensure low MRSA prevalence, it is important to investigate the likely origin of this MRSA of unknown origin (MUO). Recently, it was shown that CC398 strains originating from humans and animals differ in the presence of specific mobile genetic elements (MGEs). We hypothesized that determining these specific MGEs in MUO isolates and comparing them with a set of CC398 isolates of various known origin might provide clues to their origin. MUO CC398 isolates were compared to MRSA CC398 isolates obtained from humans with known risk factors, a MRSA CC398 outbreak isolate, livestock associated (LA) MRSA CC398 isolates from pigs, horses, chickens, and veal calves, and five methicillin-susceptibleStaphylococcus aureus(MSSA) CC398 isolates of known human origin. All strains werespatyped, and the presence or absence of,scn,chp, φ3int, φ6int, φ7int,rep7,rep27, andcadDXwas determined by PCRs. The MRSA CC398 in humans, MUO, or MRSA of known origin (MKO) resembled MRSA CC398 as found in pigs and not MSSA CC398 as found in humans. The distinct human MSSA CC398spatype, t571, was not present among our MRSA CC398 strains; MRSA CC398 was tetracycline resistant and carried no φ3 bacteriophage withscnandchp. We showed by simple PCR means that human MUO CC398 carriers carried MRSA from livestock origin, suggestive of indirect transmission. Although the exact transmission route remains unknown, direct human-to-human transmission remains a possibility as well.

scholarly journals Isolation and Host Range of Bacteriophage with Lytic Activity against Methicillin-Resistant Staphylococcus aureus and Potential Use as a Fomite Decontaminant

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0131714 ◽  
Author(s):  
Kyle C. Jensen ◽  
Bryan B. Hair ◽  
Trevor M. Wienclaw ◽  
Mark H. Murdock ◽  
Jacob B. Hatch ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Host Range ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Lytic Activity ◽  
Methicillin Resistant ◽  
Potential Use

scholarly journals Phages for methicillin-resistant Staphylococcus aureus: an international trial

1999 ◽  
Vol 122 (2) ◽  
pp. 227-233 ◽  
Author(s):  
J. F. RICHARDSON ◽  
V. T. ROSDAHL ◽  
W. J. van LEEUWEN ◽  
A. M. VICKERY ◽  
A. VINDEL ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Human Origin ◽  
Methicillin Resistant ◽  
International Trial ◽  
Resistant Strains

An internationally agreed and validated set of phages is used worldwide for the typing of strains of Staphylococcus aureus of human origin. However, because of the sometimes reduced susceptibility of methicillin-resistant strains (MRSA) to these phages, some of the national typing centres use locally isolated and characterized sets of experimental phages. In this trial, 42 such phages were distributed to 6 centres and tested against 744 isolates of MRSA with the intention of defining a phage set to augment the international set. The use of these experimental phages increased the percentage typability from 75% with the international set to 93% and the number of identifiable lytic patterns from 192 to 424. A subset of 10 experimental phages was selected. When this subset was compared with the experimental panel, the typability rate was 91% and 370 distinct patterns were obtained. This subset of phages has been distributed for international trial.

scholarly journals Characterization of vB_Sau_S90, and vB_Sau_S165, a Broad Host Range Phages, and Multiple Phage Doses as a Potential Therapeutic Strategy to Eliminate Transient Phage Resistant Mutants in Staphylococcus Aureus

2021 ◽  
Author(s):  
Archana Loganathan ◽  
Ramesh Nachimuthu
Keyword(s):  
Staphylococcus Aureus ◽  
Host Range ◽  
Serial Passage ◽  
Broad Host Range ◽  
Bacterial Cells ◽  
Bacterial Killing ◽  
Methicillin Resistant ◽  
Adsorption Time ◽  
Wide Range

Abstract Methicillin-Resistant Staphylococcus aureus is a human pathogen. MRSA has acquired resistance to major antibiotics; thus, phage therapy has become a potential alternative treatment. In this work, two broad host range Staphylococcus phages were characterized for lifecycle, physio-chemical parameters and bacterial killing kinetics, and the in vitro behavior of phage insensitive bacterial cells to alternative serial passage and multiple phage doses were assessed by reduction in the bacterial turbidity and spot assay. Phage vB_Sau_S90 showed an absorption efficiency of 91 ± 0.6% with an adsorption time of 17 ± 1 min and vB_Sau_S165 of 95 ± 0.5% adsorption efficiency and 15 ± 2 min adsorption time. Both the phages were stable over a wide range of temperature (20 to 50 ℃) and pH (3 to 11). vB_Sau_S90 phage belonging to the family Siphoviridae [order Caudovirals] showed killing efficiency against 88% (181/205) of S. aureus isolates, and vB_Sau_S165 belonging to family Podoviridae [order Caudovirals] showed killing efficiency against 94% (192/205) of S. aureus isolates. The sensitive and transient phage-resistant cells that remained uninfected during the single dose of phage treatment were eliminated upon a minimum of five alternative serial passage and multiple phage doses. This study concludes that both the phages showed promising activity against methicillin-resistant Staphylococcus aureus. Our study revealed that despite phage auto-dosing and high therapeutic efficiency, both phages did not produce a complete bacterial clearance at a single phage dose; hence indicated that multiple phage doses were required to attain a successful and complete bacterial eradication.

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