scholarly journals Detection and new genetic environment of the pleuromutilin-lincosamide-streptogramin A resistance gene lsa(E) in methicillin-resistant Staphylococcus aureus of swine origin

2013 ◽  
Vol 68 (6) ◽  
pp. 1251-1255 ◽  
Author(s):  
B. Li ◽  
S. Wendlandt ◽  
J. Yao ◽  
Y. Liu ◽  
Q. Zhang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Gene ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant ◽  
Genetic Environment

scholarly journals Identification of a Plasmid-Borne Resistance Gene Cluster Comprising the Resistance Genes erm(T), dfrK, and tet(L) in a Porcine Methicillin-Resistant Staphylococcus aureus ST398 Strain

2009 ◽  
Vol 54 (2) ◽  
pp. 915-918 ◽  
Author(s):  
Kristina Kadlec ◽  
Stefan Schwarz
Keyword(s):  
Staphylococcus Aureus ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Streptococcus Pyogenes ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Tetracycline Resistance ◽  
Sequence Type ◽  
The Novel ◽  
Methicillin Resistant ◽  
Resistance Gene Cluster

ABSTRACT A novel plasmid-borne resistance gene cluster comprising the genes erm(T) for macrolide-lincosamide-streptogramin B resistance, dfrK for trimethoprim resistance, and tet(L) for tetracycline resistance was identified in a porcine methicillin-resistant Staphylococcus aureus sequence type 398 (ST398) strain. This erm(T)-dfrK-tet(L) region was flanked by copies of the novel IS element ISSau10. The erm(T) region resembled that of Streptococcus pyogenes plasmid pRW35. The erm(T) gene of pKKS25 was expressed constitutively due to a 57-bp deletion in the erm(T) translational attenuator.

scholarly journals Copresence oftet(K) andtet(M) in Livestock-Associated Methicillin-Resistant Staphylococcus aureus Clonal Complex 398 Is Associated with Increased Fitness during Exposure to Sublethal Concentrations of Tetracycline

2016 ◽  
Vol 60 (7) ◽  
pp. 4401-4403 ◽  
Author(s):  
Jesper Larsen ◽  
Julie Clasen ◽  
Julie E. Hansen ◽  
Wilhelm Paulander ◽  
Andreas Petersen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Gene ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clonal Complex ◽  
Tetracycline Resistance ◽  
Tetracycline Resistance Gene ◽  
Methicillin Resistant ◽  
Content Type ◽  
Sublethal Concentrations ◽  
Staphylococcal Cassette Chromosome

ABSTRACTThe tetracycline resistance genetet(K) was shown to be integrated within the predominant staphylococcal cassette chromosomemec(SCCmec) element of Danish livestock-associated methicillin-resistantStaphylococcus aureusCC398 (LA-MRSA CC398). These LA-MRSA CC398 isolates already possessedtet(M), but the acquisition oftet(K) significantly improved their fitness at sublethal concentrations of tetracycline. Becausetet(K) is genetically linked to SCCmec, the use of tetracycline in food animals may have contributed to the successful spread of LA-MRSA CC398.

scholarly journals Incidence of Vancomycin Resistant Phenotype of the Methicillin Resistant Staphylococcus aureus Isolated from a Tertiary Care Hospital in Lahore

Antibiotics ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 3 ◽  
Author(s):  
Aqib Saeed ◽  
Fatima Ahsan ◽  
Muhammad Nawaz ◽  
Khadeja Iqbal ◽  
Kashif Rehman ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Gene ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Vancomycin Resistance ◽  
Diffusion Method ◽  
Care Hospital ◽  
Methicillin Resistant ◽  
Vancomycin Resistant

Staphylococcus aureus (S. aureus)-associated infections are one of the major threats to public health. The aim of the present study was to determine the antibiotic resistance pattern as well as the genetic characterization of methicillin and vancomycin resistant S. aureus (VRSA) isolated from a tertiary care hospital in Lahore. The S. aureus isolates were isolated from different clinical samples, identified by biochemical testing, and subjected to antibiotic susceptibility testing via the disc diffusion method or broth microdilution method. The methicillin resistance gene (mecA) and vancomycin resistance gene (vanA) were amplified by the polymerase chain reaction. The S. aureus isolates showed high incidences of resistance against methicillin (76%) and moderate incidences of resistance to vancomycin (14%). Isolates were also resistant to several other drugs, such as cefoxitin (76%), ertapenem (83%), ampicillin (81%), tobramycin (78%), moxifloxacin (76%), and tetracycline (74%). An encouraging finding was that 98% of isolates were susceptible to tigecycline, indicating its possible role in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) and VRSA, as well as the multi-drug resistant S. aureus. The mecA gene was detected in 33.3% of tested isolates (10/30), while the vanA gene was also detected in 30% (9/30) of the tested isolates. In conclusion, the frequent presence of methicillin and vancomycin resistance in S. aureus appraises the cautious use of these antibiotics in clinical practices. Furthermore, it is suggested that there should be continuous monitoring of tigecycline treatments in clinical setups in order to delay the development of resistance against it.

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