Simultaneous Assay for Six Alkaloids in Opium, Using High-Performance Liquid Chromatography

1975 ◽  
Vol 58 (5) ◽  
pp. 888-897
Author(s):  
Howard W Ziegler ◽  
Thomas H Beasley ◽  
David W Smith
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Internal Standard ◽  
Dilute Acid ◽  
Reduced Pressure ◽  
Double Beam ◽  
Flow Through ◽  
Chloroform Methanol

Abstract A method is described for the quantitative determination of morphine, codeine, cryptopine, thebaine, papaverine, and narcotine in opium by high-performance liquid chromatography. The alkaloids are isolated from a dilute acid extract by adsorption on an Amberlite XAD-2 resin column and eluted first with methanol and then with chloroform-methanol ( 3+1 ) . After solvent removal by reduced pressure evaporation, the alkaloids are redissolved in chloroformmethanol ( 3+1 ) . The sample solution, plus brucine as an internal standard, is injected onto a Corasil II column and eluted with hexane that is gradient programmed with a solution of chloroform-methanol-diethylamine ( 100+300+1). The absorbances of the separated alkaloids are continuously monitored at 254 nm, using a flow-through ultraviolet double-beam photometer.

Determination of cocaine concentrations in plasma by high-performance liquid chromatography

1990 ◽  
Vol 36 (8) ◽  
pp. 1436-1439 ◽  
Author(s):  
P Jatlow ◽  
H Nadim
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Plasma Concentrations ◽  
Internal Standard ◽  
Reversed Phase ◽  
Dilute Acid ◽  
Nitrogen Detection ◽  
Chromatographic Procedure

Abstract We describe a procedure for measuring concentrations of cocaine in plasma by reversed-phase high-performance liquid chromatography, with ion-pairing. The procedure involves solvent extraction followed by back-extraction with dilute acid. The n-propyl ester of benzoylecgonine is used as an internal standard. An evaporation step is not required, and concentrations as low as 5 micrograms/L can be quantified. Plasma concentrations of cocaine determined by high-performance liquid chromatography correlated well with those determined by a previously reported gas-chromatographic procedure with nitrogen detection.

scholarly journals A Simple High-Performance Liquid Chromatography Method for Simultaneous Determination of Three Triazole Antifungals in Human Plasma

2012 ◽  
Vol 57 (1) ◽  
pp. 484-489 ◽  
Author(s):  
Mei Zhang ◽  
Grant A. Moore ◽  
Murray L. Barclay ◽  
Evan J. Begg
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
High Performance ◽  
Internal Standard ◽  
Gradient Elution ◽  
Limit Of Quantification ◽  
Chromatography Method

ABSTRACTA rapid and simple high-performance liquid chromatography (HPLC) assay was developed for the simultaneous determination of three triazole antifungals (voriconazole, posaconazole, and itraconazole and the metabolite of itraconazole, hydroxyitraconazole) in human plasma. Sample preparation involved a simple one-step protein precipitation with 1.0 M perchloric acid and methanol. After centrifugation, the supernatant was injected directly into the HPLC system. Voriconazole, posaconazole, itraconazole, its metabolite hydroxyitraconazole, and the internal standard naproxen were resolved on a C6-phenyl column using gradient elution of 0.01 M phosphate buffer, pH 3.5, and acetonitrile and detected with UV detection at 262 nm. Standard curves were linear over the concentration range of 0.05 to 10 mg/liter (r2> 0.99). Bias was <8.0% from 0.05 to 10 mg/liter, intra- and interday coefficients of variation (imprecision) were <10%, and the limit of quantification was 0.05 mg/liter.

Simplified micro-scale procedure for preparing samples for theophylline determination by liquid chromatography.

1977 ◽  
Vol 23 (1) ◽  
pp. 124-126 ◽  
Author(s):  
J W Nelson ◽  
A L Cordry ◽  
C G Aron ◽  
R A Bartell
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Standard Sample ◽  
Retention Volume ◽  
Internal Standard ◽  
Reverse Phase ◽  
Micro Scale ◽  
Quantitative Results

Abstract We describe an improved procedure for the preparation of plasma or serum for determination of theophylline by reverse phase high-performance liquid chromatography. Quantitative results are available in less than 30 min from receipt of sample. The chromatogram is complete in 8 to 16 min, which includes the use of an internal standard. Sample preparation consists of simple solvent denaturation of the sample proteins, and centrifugation to remove protein before chromatography. No precolumn is required to pretect the separating column. No interference was noted when sodium or lithium heparin or ethylenediaminetetraacetate were used as anticoagulants, but citrate treatment proved to be unsatisfactory because of a highly absorbing band that eluted with the same retention volume as theophylline.

scholarly journals Relevance of Matrix Effect in Determination of Biogenic Amines in Plaice (Pleuronectes platessa) and Whiting (Merlangus merlangus)

1999 ◽  
Vol 82 (5) ◽  
pp. 1097-1101 ◽  
Author(s):  
Guillaume Duflos ◽  
Catherine Dervin ◽  
Pierre Malle ◽  
Stephane Bouquelet
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Biogenic Amines ◽  
Muscle Tissue ◽  
Biogenic Amine ◽  
Matrix Effect ◽  
High Performance ◽  
Internal Standard ◽  
Tissue Matrix

Abstract Spoilage can be evaluated by separating and determining biogenic amines by various techniques, notably high-performance liquid chromatography. Previous studies have not taken into account how the muscle tissue matrix affects the assay. We demonstrate a matrix effect in plaice and whiting and show that it changes during spoilage. This effect should be taken into account when plotting regression lines relating the quantity of amine to the biogenic amine/internal standard ratio.

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