High Performance Liquid Chromatographic Determination of Phenothiazine Residues in Sheep Tissues

1980 ◽  
Vol 63 (5) ◽  
pp. 988-991
Author(s):  
Graeme L Blackman ◽  
Ah Chai Ho ◽  
Alex Jozsa ◽  
John D Kelly
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Tissue Samples ◽  
Silica Column ◽  
Liquid Chromatographic Determination ◽  
Residue Levels ◽  
Liquid Chromatographic

Abstract A high performance liquid chromatographic (HPLC) technique is described for the determination of residue levels of the anthelmintic drug phenothiazine in sheep tissues. Phenothiazine was administered to sheep which were slaughtered after withholding periods of 24, 48, and 72 h. Residues of phenothiazine were then extracted from tissue samples by homogenization in methanol. The HPLC analysis of the extracts involved separation on a 10 μm silica column using a mobile phase of 0.3% n-propanol in cyclohexane. The lower limit of detection by ultraviolet absorption at 254 nm was 0.05 ppm

Liquid-chromatographic determination of alpha- and gamma-tocopherols in erythrocytes, with fluorescence detection.

1983 ◽  
Vol 29 (10) ◽  
pp. 1840-1842 ◽  
Author(s):  
J Lehmann ◽  
H L Martin
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract We have adapted to erythrocytes a method for the determination of alpha- and gamma-tocopherols in plasma and platelets. Erythrocytes (50 microL) were extracted with methanol containing tocol (internal standard) and pyrogallol. Tocopherols were partitioned into chloroform, washed, and injected in methanol onto a reversed-phase (C18) "high-performance" liquid-chromatographic column. The mobile phase was methanol/water (99/1 by vol) at a flow rate of 2 mL/min and detection was with a "high-performance" spectrophotofluorometer. The limit of detection for either tocopherol is 0.10 microgram/mL of packed cells. Analytical recoveries ranged from 93 to 104%. Some values for tocopherols in human erythrocytes are presented.

High Performance Liquid Chromatographic Determination of Lactose in Milk

1981 ◽  
Vol 64 (4) ◽  
pp. 805-807
Author(s):  
Leslie G West ◽  
Marie A Llorente
Keyword(s):  
High Performance ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Chocolate Milk ◽  
Silica Column ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A rapid and simple high performance liquid chromatographic method for the determination of lactose in milk was developed. Samples were diluted with 0.5% perchloric acid and centrifuged, and an aliquot of the supernate was mixed with acetonitrile. Lactose was separated on a 10 μm particle-size silica column with aqueous acetonitrile as the mobile phase. The recovery of lactose from whole, skim, and chocolate milk averaged 99.2, 101.1, and 100.4%, respectively. Coefficients of variation for routinely performed duplicate determinations are between 1.0 and 1.5%.

Ion-Pair High Performance Liquid Chromatographic Determination of Inosinic Acid in Meat

1983 ◽  
Vol 66 (3) ◽  
pp. 632-634
Author(s):  
Yoshimi Kitada ◽  
Masashige Inoue ◽  
Kikuo Tamase ◽  
Masako Imou ◽  
Akikazu Hasuike ◽  
...  
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Ion Pair ◽  
Inosinic Acid ◽  
Tetrabutyl Ammonium ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract An ion-pair high performance liquid chromatographic (HPLC) technique is described for the determination of inosinic acid (IMP) in meat. The compound was extracted with perchloric acid and analyzed without cleanup. IMP is effectively separated, identified, and quantitated by using a reverse phase column, with ultraviolet detection. A C8 stationary phase and tetrabutyl ammonium as counter ion are used. Recovery of IMP added to meat at 500 or 2500 ppm levels was more than 95%; the limit of detection for IMP is 50 ppm.

High Performance Liquid Chromatographic Determination of Bendiocarb on Wool

1980 ◽  
Vol 63 (1) ◽  
pp. 47-48
Author(s):  
James M Zehner ◽  
Richard A Simonaitis ◽  
Roy E Bry
Keyword(s):  
Standard Deviation ◽  
High Performance ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Uv Detector ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A high performance liquid chromotographic method is presented for determining bendiocarb (2,2-dimethyl-1,3-benzodioxol-4-yl methylcarbamate) on wool. Bendiocarb is extracted from wool with methanol containing methyl benzoate as internal standard, eluted through a Zorbax ODS column with methanol-water (55 + 45), and detected with a UV detector at 280 nm. The method can be used to determine bendiocarb at 0.001–0.02% by weight. The limit of detection is 0.0004%, or 4 ppm. At 4 analyses each, recovery at 0.013% was 101%, standard deviation 2.8%; at 0.003%, recovery was 96%, standard deviation 5.6%; at 0.001%, recovery was 103%, standard deviation 2.9%.

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