Analytical Methods for Determining Ascorbic Acid in Biological Samples, Food Products, and Pharmaceuticals

Abstract Over the last decade, numerous publications have appeared describing \ analyses for ascorbic acid in food products, pharmaceuticals, and biological samples. This review focuses on the chemistry associated with many of these procedures. The papers discussed have historical importance, are important to understanding the method, or have significantly advanced ascorbic acid analysis. The review has 4 major, sections: spectroscopic, electrochemical, enzymatic, and chromatographic methods of analysis.

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Analytical Methods for Measuring Uric Acid in Biological Samples and Food Products

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/70.1.1 ◽

1987 ◽

Vol 70 (1) ◽

pp. 01-14 ◽

Cited By ~ 1

Author(s):

Lawrence A Pachla ◽

Donald L Reynolds ◽

D Scott Wright ◽

Peter T Kissinger

Keyword(s):

Uric Acid ◽

Analytical Method ◽

Biological Samples ◽

Analytical Methods ◽

State Of The Art ◽

Food Products ◽

Chemical Processes ◽

Advantages And Disadvantages ◽

Chromatographic Methods ◽

Unique Chemical

Abstract Daring the last 7 decades, uric acid methodology has kept pace with the introduction of state-of-the-art technology (e.g., spectroscopy, electrochemistry, chromatography) or the discovery of unique chemical processes (e.g., redox, enzymatic). We envision this practice will continue in the future. There never will be a single analytical method applicable for biofluids or foodstuffs. Therefore, it is imperative that the analyst not only understand the advantages and disadvantages of a procedure, but also thoroughly understand its underlying chemical and technological principles. Since many procedures available for analysis of biofluids and foodstuffs rely on identical chemical or technological principles, this report shall review both sample types and the available spectroscopic, electroanalytical, and chromatographic methods

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Validationof chromatographic methods of analysis of drugs in biological samples

TrAC Trends in Analytical Chemistry ◽

10.1016/0165-9936(86)85042-7 ◽

1986 ◽

Vol 5 (5) ◽

pp. 115-117 ◽

Cited By ~ 19

Author(s):

Darioush Dadgar ◽

Malcolm R. Smyth

Keyword(s):

Biological Samples ◽

Chromatographic Methods ◽

Methods Of Analysis

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Measurement of ascorbic acid in human plasma and serum: stability, intralaboratory repeatability, and interlaboratory reproducibility

Clinical Chemistry ◽

10.1093/clinchem/42.8.1257 ◽

1996 ◽

Vol 42 (8) ◽

pp. 1257-1262 ◽

Cited By ~ 49

Author(s):

S A Margolis ◽

D L Duewer

Keyword(s):

Ascorbic Acid ◽

Human Plasma ◽

Analytical Methods ◽

Dehydroascorbic Acid ◽

Metaphosphoric Acid ◽

Serum Stability ◽

Chromatographic Methods ◽

Measurement Reproducibility ◽

Stability Data ◽

Interlaboratory Reproducibility

Abstract We demonstrate that total ascorbic acid (TAA, the sum of ascorbic acid and dehydroascorbic acid) in properly prepared human plasma is stable at -70 degrees C for at least 6 years when preserved with dithiothreitol. TAA in human plasma or serum preserved with metaphosphoric acid degrades slowly, at the rate of no more than 1% per year. As assessed from our stability data and from data obtained from 23 laboratories over a period of > 2 years, the intralaboratory repeatability of TAA measurement is approximately 2 mumol/L, irrespective of TAA concentration. Nonchromatographic analytical methods involving dinitrophenylhydrazine and 0-phenylenediamine yield biased results relative to chromatographic methods. Within groups of laboratories that use roughly similar analytical methods, the interlaboratory measurement reproducibility CV for TAA is 15%.

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