Rapid Screening Method for Detection of Deoxynivalenol

1985 ◽  
Vol 68 (6) ◽  
pp. 1126-1128 ◽  
Author(s):  
Gail M Shannon ◽  
Robert E Peterson ◽  
Odette L Shotwell
Keyword(s):  
Environmental Samples ◽  
Chloride Solution ◽  
Screening Method ◽  
Response Characteristic ◽  
Rapid Screening ◽  
Potassium Chloride Solution ◽  
Reverse Phase ◽  
Hydrophilic Matrix ◽  
Rapid Screening Method ◽  
Layer Chromatography

Abstract A rapid method for detecting deoxynivalenol (DON), also known as vomitoxin, was developed. DON was extracted from grains and other samples with acetonitrile-4% potassium chloride solution (9 + 1). Impurities that would interfere with detection were removed on a Ci8 silica gel reverse phase column. Water was removed from eluates on a hydrophilic matrix column. DON was detected by thin layer chromatography using an aluminum chloride solution to develop the blue response characteristic of the mycotoxin. Total time involved is approximately 30 min. The method was applicable to corn, wheat, and barley at detection levels of 1 ppm, and oats at 1.5 ppm. It is applicable to environmental samples (soil, green plants, and water) at detection levels of 0.75 ppm.

Rapid Screening Method for Deoxynivalenol in Agricultural Commodities by Fluorescent Minicolumn

1990 ◽  
Vol 73 (2) ◽  
pp. 266-270
Author(s):  
William C Gordon ◽  
Linda J Gordon
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Selective Adsorption ◽  
Screening Method ◽  
Rapid Screening ◽  
Blue Fluorescence ◽  
False Negatives ◽  
Rapid Screening Method ◽  
Gas Chromatography Mass Spectroscopy ◽  
Layer Chromatography

Abstract A rapid screening procedure based on the selective adsorption of deoxynivalenol (DON) from extracts of wheat and corn has been developed. DON is extracted from the sample with acetonitrile-water (85 + 15) and partially purified on a preparative minicolumn. Solvent is evaporated and the residue is dissolved in toluene-acetone (95 + 5) and chromatographed on a novel detector minicolumn which selectively adsorbs DON. A blue fluorescence is produced when the column is heated 5 min at 100°C. The procedure is capable of detecting DON at ≥ 500 ng/g. Forty-three wheat samples, contaminated with DON at 60-6300 ng/g, were assayed by gas chromatography-mass spectroscopy (GC-MS) of the heptafluorobutyryl derivative of DON and by the selective adsorption procedure. Comparison of results showed 9 1% agreement between data from the 2 methods. Selective adsorption assays were positive for all samples that were ≥ 500 ng/g by GC-MS (no false negatives) and were negative for 85 % of samples < 500 ng/g (4/27 false positives). These four samples contained > 200 ng/g by GC-MS. Samples of wheat (64), corn (23), soybeans (8), and sorghum (6) were extracted and extracts were assayed by thin-layer chromatography and the selective adsorption procedure. Selective adsorption assays agreed with TLC results.

Thin-Layer Chromatography as a Rapid Screening Method for the Determination of Carbaryl and 1-Naphthol Residues Without Cleanup

1964 ◽  
Vol 47 (4) ◽  
pp. 667-670
Author(s):  
M Chiba ◽  
H V Morley
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Screening Method ◽  
Rapid Screening ◽  
Detection And Estimation ◽  
Rapid Screening Method ◽  
Layer Chromatography

Abstract A rapid, sensitive, screening method has been developed in which thin-layer chromatography is used for the detection and estimation of carbaryl and l-naphthol residues in apple, lettuce, and tomato extracts without prior cleanup. A procedure giving a convenient screening range of 0.1—30 ppm is described, but 0.02 ppm in apples and lettuce and 0.03 ppm in tomatoes may be detected without cleanup. By use of a partitioning cleanup 5 ppb of carbaryl may be detected.

Development of a simple and rapid screening method for the detection of 1-(3-chlorophenyl)piperazine in forensic samples

Talanta ◽  
2021 ◽  
pp. 122597
Author(s):  
Weberson P. Silva ◽  
Raquel G. Rocha ◽  
Luciano C. Arantes ◽  
Camila D. Lima ◽  
Larissa M.A. Melo ◽  
...  
Keyword(s):  
Screening Method ◽  
Rapid Screening ◽  
Forensic Samples ◽  
Rapid Screening Method
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