scholarly journals Simultaneous Determination of Chlorpheniramine Maleate and Dexamethasone in a Tablet Dosage Form by Liquid Chromatography

2005 ◽  
Vol 88 (6) ◽  
pp. 1677-1683 ◽  
Author(s):  
María A Moyano ◽  
María A Rosasco ◽  
María T Pizzorno ◽  
Adriana I Segall
Keyword(s):  
Potassium Phosphate ◽  
Reversed Phase ◽  
Chlorpheniramine Maleate ◽  
Constant Flow Rate ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Tablet Dosage ◽  
Liquid Chromatographic ◽  
Interday Precision

Abstract An accurate, simple, reproducible, and sensible liquid chromatographic method was developed and validated for the determination of chlorpheniramine maleate and dexamethasone in a tablet formulation. The analysis was performed at room temperature on a reversed-phase C18 column with UV detection at 254 nm. The mobile phase consisted of 7.5 mM monobasic potassium phosphate in methanol–water (62.5 + 37.5) at a constant flow rate of 1 mL/min. The method was validated in terms of linearity, precision, accuracy, and specificity by forced decomposition of chlorpheniramine maleate and dexamethasone initiated by using acid, base, water, hydrogen peroxide, heat, and light. The response was linear in the ranges of 0.04–0.12 and 0.006–0.016 mg/mL for chlorpheniramine maleate (r2 = 0.9999) and dexamethasone (r2 = 0.9994), respectively. The relative standard deviation values for intra- and interday precision studies were 2.39 and 2.02, respectively, for chlorpheniramine maleate and 2.39 and 1.25, respectively, for dexamethasone. Recoveries ranged from 95.07 to 101.95% for chlorpheniramine maleate and from 97.75 to 102.10% for dexamethasone.

scholarly journals Determination of Omeprazole in Bulk and Injectable Preparations by Liquid Chromatography

2003 ◽  
Vol 86 (3) ◽  
pp. 501-504 ◽  
Author(s):  
Alexandre Schubert ◽  
Almeci L Werle ◽  
Cleber A Schmidt ◽  
Cristiane Codevilla ◽  
Lisiane Bajerski ◽  
...  
Keyword(s):  
Room Temperature ◽  
Reversed Phase ◽  
Constant Flow ◽  
Uv Detector ◽  
Constant Flow Rate ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic ◽  
Interday Precision

Abstract An accurate, simple, reproducible, and sensitive liquid chromatographic method was developed and validated for the determination of omeprazole in powder for injection and in pellets. The analyses were performed at room temperature on a reversed-phase C18 column of 250 × 4.6 mm id, 5 μm particle size. The mobile phase, composed of methanol–water (90 + 10, v/v), was pumped at a constant flow rate of 1.5 mL/min. Detection was performed on a UV detector at 301 nm. The method was validated in terms of linearity, precision, accuracy, and ruggedness. The response was linear in the range 32–48 μg/mL (r2 = 0.9976). The relative standard deviation values for intra-and interday precision studies were 1.22 and 1.56% for injectable and 2.13 and 2.45% for pellets, respectively. Recoveries ranged between 95.81 and 100.48%.

scholarly journals Validation of a Liquid Chromatographic Method for Determination of Tacrolimus in Pharmaceutical Dosage Forms

2006 ◽  
Vol 89 (6) ◽  
pp. 1547-1551 ◽  
Author(s):  
MarÍa A Moyano ◽  
Laura D Simionato ◽  
Mara T Pizzorno ◽  
Adriana I Segall
Keyword(s):  
Room Temperature ◽  
Chromatographic Method ◽  
Reversed Phase ◽  
Pharmaceutical Dosage Forms ◽  
Constant Flow Rate ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic ◽  
Interday Precision

Abstract An accurate, simple, and reproducible liquid chromatographic method was developed and validated for the determination of tacrolimus in capsules. The analysis is performed at room temperature on a reversed-phase C18 column with UV detection at 210 nm. The mobile phase is methanolwater (90 10) at a constant flow rate of 0.8 mL/min. The method was validated in terms of linearity, precision, accuracy, and specificity by forced decomposition of tacrolimus, using acid, base, water, hydrogen peroxide, heat, and light. The response was linear in the range of 0.090.24 mg/mL (r2 0.9997). The relative standard deviation values for intra- and interday precision studies were 1.28 and 2.91%, respectively. Recoveries ranged from 98.06 to 102.52%.

scholarly journals Validated RP-HPLC Method for Analysis of Aripiprazole in a Formulation

2010 ◽  
Vol 7 (3) ◽  
pp. 827-832 ◽  
Author(s):  
R. Kalaichelvi ◽  
B. Thangabalan ◽  
D. Srinivasa Rao
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Hplc Method ◽  
Rp Hplc ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Tablet Dosage ◽  
Liquid Chromatographic

A rapid, simple and validated reversed-phase high-performance liquid chromatographic method has been developed for analysis of aripiprazole in tablet dosage form. Aripiprazole was separated on an ODS analytical column with a 40:60 (v/v) mixture of acetonitrile and triethanolamine buffer (5 mM, pH 3.5 ± 0.05 adjusted by addition of 85% phosphoric acid) as mobile phase at a flow rate of 1.5 mL min-1. The effluent was monitored by UV detection at 254 nm. Calibration plots were linear in the range of 20 to 60 µg mL-1and the LOD and LOQ were 0.411 and 1.248 µg mL-1, respectively. The high recovery and low relative standard deviation confirm the suitability of the method for routine quality control determination of aripiprazole in tablets.

scholarly journals Application of Liquid Chromatography to the Simultaneous Determination of Acetylsalicylic Acid, Caffeine, Codeine, Paracetamol, Pyridoxine, and Thiamine in Pharmaceutical Preparations

2001 ◽  
Vol 84 (3) ◽  
pp. 676-683 ◽  
Author(s):  
Natividad Ramos-Martos ◽  
Francisco Aguirre-Gómez ◽  
Antonio Molina-Díaz ◽  
Luis F Capitán-Vallvey
Keyword(s):  
Salicylic Acid ◽  
Acetylsalicylic Acid ◽  
Simultaneous Determination ◽  
Pharmaceutical Preparations ◽  
Reversed Phase ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract This paper describes a rapid reversed-phase liquid chromatographic method, with UV detection, for the simultaneous determination of acetylsalicylic acid, caffeine, codeine, paracetamol, pyridoxine, and thiamine in pharmaceutical preparations. A reversed-phase C18 Nucleosil column is used. The mobile phase consists of 2 successive eluants: water (5 min) and acetonitrile–water (75 + 25, v/v; 9 min), both adjusted to pH 2.1 with phosphoric acid. Before determination acetylsalicylic acid is completely converted to salicylic acid by alkaline hydrolysis. Salicylic acid, caffeine, paracetamol, pyridoxine, and thiamine are all detected at 285 nm, whereas codeine is detected at 240 nm. Calibration curves were linear for salicylic acid, caffeine, paracetamol, and pyridoxine in the range of 50–500 mg/L, and for codeine and thiamine in the range of 50–1000 mg/L. The method was applied to the analysis of 13 fortified commercial pharmaceutical preparations. Recoveries ranged from 92.6 to 105.5%, with relative standard deviations of 1.1–5.8%.

scholarly journals Simultaneous Determination of Bioactive Xanthone Glycosides and Norlignans from Ethanolic Extract of Anemarrhena asphodeloides by Liquid Chromatography

2008 ◽  
Vol 91 (6) ◽  
pp. 1271-1277 ◽  
Author(s):  
M Nurul Islam ◽  
Hye Hyun Yoo ◽  
Jun Lee ◽  
Joo Won Nam ◽  
Eun Kyoung Seo ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Reversed Phase ◽  
Linear Behavior ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Aggregation Inhibition ◽  
Phase Liquid ◽  
Liquid Chromatographic ◽  
Anemarrhena Asphodeloides

Abstract The rhizomes of Anemarrhena asphodeloides Bunge (Liliaceae) are prescribed as crude drugs in herbal medication for the treatment of various diseases such as diabetes, inflammation, and platelet aggregation inhibition. A simple, sensitive, and precise reversed-phase liquid chromatographic method was developed to study the quantitative determination of 5 bioactive compounds from these rhizomes, namely, neomangiferin, mangiferin, isomangiferin, nyasol, and methylnyasol. Chromatographic analysis was performed on Capcell Pak C18 column (150 4.6 mm, 3 m) with a mobile phase consisting of acetonitrile, methanol, and 0.1 formic acid at a flow rate of 1.00 mL/min. Quantitation was performed using a UV-visible detector at 260 nm. The method for the determination of reported medicinal agents was accurate and reproducible. Excellent linear behavior was observed over the investigated concentration range of 2.5100.0 g/mL for neomangiferin; 1.560.0 g/mL for mangiferin; 0.520.0 g/mL for nyasol; and 0.220.0 g/mL for methylnyasol; correlation coefficient >0.99. The intraday and interday precision over the concentration range of compounds was <6.6 (relative standard deviation) and accuracy was between 94.9 and 109.3. This method can be successfully applied for the analysis of medicinal compounds from the ethanolic extract of A. asphodeloides Bunge.

Comparison of Liquid Chromatographic Method to AOAC Microbiological Method for Determination of L-Tryptophan in Tablets and Capsules

1993 ◽  
Vol 76 (2) ◽  
pp. 414-417 ◽  
Author(s):  
Henry S Kim ◽  
Gerald Angyal
Keyword(s):  
Reversed Phase ◽  
Precolumn Derivatization ◽  
Microbiological Method ◽  
Test Portion ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Phase Liquid ◽  
The Mean ◽  
Liquid Chromatographic

Abstract A reversed-phase liquid chromatographic (LC) method coupled with precolumn derivatization of L-tryptophan with phenylisothiocyanate was compared to the AOAC microbiological method for determining L-tryptophan in tablets and capsules. For the microbiological method, the concentrations of L-tryptophan were 4-8% lower in autoclaved test samples (hot method) than in test samples that were not autoclaved (cold method). When L-tryptophan values obtained by the LC method were compared to those obtained by the cold microbiological method, no significant differences were observed (P > 0.05). The mean relative standard deviations were 2.9% for the LC method and 1.6% for the cold microbiological method. The mean recoveries of standard L-tryptophan added before analysis were 99% for the LC method and 101 % for the cold microbiological method. These results demonstrate that both methods are reliable for determining free L-tryptophan contained in tablets and capsules. However, the LC method has the advantages of using a smaller test portion and having a shorter analysis time.

Gradient Multipeak Liquid Chromatographic Method for Determination of Ardacin in Bulk Chemical and Premix Formulations

1995 ◽  
Vol 78 (2) ◽  
pp. 289-293
Author(s):  
Hafez Abdel-Kader ◽  
Myriam M Kobylkevich ◽  
Larry S Wigman ◽  
Govind K Menon
Keyword(s):  
Pilot Scale ◽  
Reversed Phase ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
External Standard ◽  
Bulk Chemical ◽  
Method Accuracy ◽  
Liquid Chromatographic ◽  
Reversed Phase Lc

Abstract A liquid chromatographic (LC) method was developed for the determination of ardacin in bulk chemical (78 to 100%, w/w anhydrous) and premix formulations (3.3 to 26.4%, w/w). The method is based on reversed-phase LC resolution of ardacin components and detection by UV absorbance at 220 nm. Ardacin has 10 components, and each component can be quantitated separately. Total ardacin is determined by summing the areas of the 10 component peaks. Calculations are performed using an external standard approach. The method is linear for ardacin at 50 to 150 μ/mL. The method accuracy for a typical bulk chemical is ± 1.5%, w/w (relative standard deviation [RSD], 1.6%), and recovery from a typical pilot scale premix is 99.9% (RSD, 3.4%). The method is useful for monitoring stability during storage.

scholarly journals Simple and Rapid Liquid Chromatography Method for Determination of Rifabutin in Plasma

2013 ◽  
Vol 9 (2) ◽  
pp. 26-29 ◽  
Author(s):  
AK Hemanth Kumar ◽  
V Sudha ◽  
Geetha Ramachandran
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Pharmacokinetic Studies ◽  
Chromatography Method ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatography Method ◽  
Liquid Chromatographic

A high performance liquid chromatographic method for determination of rifabutin in human plasma was  developed. The method involved deproteinisation of the sample with acetonitrile and analysis of the  supernatant using a reversed-phase C18 column (250mm) and UV detection at a wavelength of 265nm.  The assay was specific for rifabutin and linear from 0.025 to 10.0μg/ml. The relative standard deviation  of intra- and inter-day assays was lower than 10%. The method was able to remove interfering materials  in plasma, yielding an average recovery of rifabutin from plasma of 101%. Due to its simplicity, the assay  can be used for pharmacokinetic studies of rifabutin. SAARC Journal of Tuberculosis, Lung Diseases & HIV/AIDS; 2012; IX(2) 26-29 DOI: http://dx.doi.org/10.3126/saarctb.v9i2.7975

scholarly journals Liquid Chromatographic Determination of the Glycoalkaloids α-Solanine and α-Chaconine in Potato Tubers: NMKL Interlaboratory Study

1997 ◽  
Vol 80 (3) ◽  
pp. 549-554 ◽  
Author(s):  
Karl-Erik Hellenás ◽  
Carina Branzell ◽  
H Poutanen ◽  
T Suortti ◽  
R Kaario ◽  
...  
Keyword(s):  
Collaborative Study ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Potato Tubers ◽  
Photometric Detection ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Twelve laboratories participated in a collaborative study to evaluate precision parameters of a liquid chromatographic method for analysis of the glycoalkaloids α-solanine and α-chaconine in potato tubers. Samples consisted of frozen potato tuber homogenates distributed as 3 blind duplicates and 3 split-level pairs. The analytical method included aqueous extraction, workup on disposable solidphase extraction cartridges, and reversed-phase chromatography with photometric detection at 202 nm. Results for α-solanine and α-chaconine were received from 10 and 9 laboratories, respectively. Relative standard deviations for reproducibilo ity for α-solanine and α-chaconine were similar, ranging from 8 to 13% in the applied concentration range of 12 to 260 mg/kg fresh weight.

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