scholarly journals Comparative Validation Study to Demonstrate the Equivalence of a Minor Modification to AOAC Method 999.09 Visual Immunoprecipitate (VIP®) for Salmonella Method to the Reference Culture Method

2009 ◽  
Vol 92 (5) ◽  
pp. 1426-1431 ◽  
Author(s):  
Philip T Feldsine ◽  
David E Kerr ◽  
George Shen ◽  
Andrew H Lienau
Keyword(s):  
Culture Method ◽  
Official Method ◽  
Comparison Study ◽  
Culture Methods ◽  
Aoac Method ◽  
Aoac Official ◽  
A Minor ◽  
And Control ◽  
Comparative Validation ◽  
Aoac Official Method

Abstract The Visual Immunoprecipitate (VIP®) for the Detection of Salmonella in Foods, AOAC Official Method 999.09, has been modified to change the color of the test and control lines of the device. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Three foods were analyzed. In total, there were valid results from 155 samples and controls. Results showed that the modified VIP Gold® for Salmonella is equivalent to the reference culture methods for the detection of Salmonella.

scholarly journals Comparative Validation Study to Demonstrate the Equivalence of a Minor Modification to AOAC Method 996.09 [Visual Immunoprecipitate (VIP®) for E. coli O157:H7] to the Reference Culture Methods

2008 ◽  
Vol 91 (2) ◽  
pp. 360-364
Author(s):  
Philip T Feldsine ◽  
David E Kerr ◽  
George S Shen ◽  
Andrew H Lienau
Keyword(s):  
Culture Method ◽  
Official Method ◽  
Comparison Study ◽  
Culture Methods ◽  
E Coli ◽  
Aoac Method ◽  
Aoac Official ◽  
A Minor ◽  
Comparative Validation ◽  
Aoac Official Method

Abstract The Visual Immunoprecipitate (VIP®)for the Detection of E. coli O157:H7 in Foods, AOAC Official Method 996.09, has been modified to use a simplified plastic housing for the device. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Three foods were analyzed. In total, valid results were obtained from 240 samples and controls. Results showed that the VIP for E. coli O157:H7 is equivalent to the reference culture methods for the detection of E. coli O157:H7.

scholarly journals Comparative Validation Study to Demonstrate the Equivalence of a Minor Modification to AOAC Method 999.09 [Visual Immunoprecipitate (VIP®) for Salmonella] to the Reference Culture Methods

2008 ◽  
Vol 91 (2) ◽  
pp. 370-375 ◽  
Author(s):  
Philip T Feldsine ◽  
David E Kerr ◽  
George S Shen ◽  
Andrew H Lienau
Keyword(s):  
Validation Study ◽  
Culture Method ◽  
Official Method ◽  
Comparison Study ◽  
Culture Methods ◽  
Aoac Method ◽  
Aoac Official ◽  
A Minor ◽  
Comparative Validation ◽  
Aoac Official Method

Abstract The Visual Immunoprecipitate (VIP®) for the Detection of Salmonella in Foods, AOAC Official Method 999.09, has been modified to use a simplified housing for the device. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Three foods were analyzed. In total, valid results were obtained from 125 samples and controls. Results showed that the modified VIP for Salmonella is equivalent to the reference culture methods for the detection of Salmonella.

scholarly journals Comparative Validation Study to Demonstrate the Equivalence of a Minor Modification to AOAC Method 997.03 [Visual Immunoprecipitate (VIP®) for Listeria] to the Reference Culture Methods

2008 ◽  
Vol 91 (2) ◽  
pp. 365-369 ◽  
Author(s):  
Philip T Feldsine ◽  
David E Kerr ◽  
George S Shen ◽  
Andrew H Lienau
Keyword(s):  
Official Method ◽  
Comparison Study ◽  
Culture Methods ◽  
Environmental Surfaces ◽  
Environmental Surface ◽  
Aoac Method ◽  
Aoac Official ◽  
A Minor ◽  
Comparative Validation ◽  
Aoac Official Method

Abstract The Visual Immunoprecipitate (VIP®) for the Detection of Listeria in Foods and Environmental Surfaces, AOAC Official Method 997.03, has been modified to use a simplified housing for the device. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture methods. Two food matrixes and one environmental surface were analyzed. In total, valid results were obtained from 145 samples and controls. Results showed that the modified VIP for Listeria spp. is equivalent to the reference culture methods for the detection of Listeria.

scholarly journals Comparative Validation Study to Demonstrate the Equivalence of a Minor Modification to AOAC Method 996.09 Visual Immunoprecipitate (VIP®) for E. coli O157:H7 Method to the Reference Culture Method

2009 ◽  
Vol 92 (5) ◽  
pp. 1416-1420
Author(s):  
Philip T Feldsine ◽  
David E Kerr ◽  
George Shen ◽  
Andrew H Lienau
Keyword(s):  
Culture Method ◽  
Official Method ◽  
Escherichia Coli O157 ◽  
Culture Methods ◽  
E Coli ◽  
Aoac Method ◽  
Aoac Official ◽  
A Minor ◽  
And Control ◽  
Comparative Validation

Abstract The Visual Immunoprecipitate (VIP®) for the Detection of Escherichia coli O157:H7 in Foods, AOAC Official Method 996.09, has been modified to change the color of the test and control lines of the device. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Three foods were analyzed. In total, there were valid results from 225 samples and controls. Results showed that the VIP Gold® for E. coli O157:H7 is equivalent to the reference culture methods for the detection of E. coli O157:H7.

scholarly journals Comparative Validation Study to Demonstrate the Equivalence of a Minor Modification to AOAC Method 997.03 Visual Immunoprecipitate (VIP®) for Listeria to the Reference Culture Method

2009 ◽  
Vol 92 (5) ◽  
pp. 1421-1425
Author(s):  
Philip T Feldsine ◽  
David E Kerr ◽  
George Shen ◽  
Andrew H Lienau
Keyword(s):  
Culture Method ◽  
Official Method ◽  
Culture Methods ◽  
Environmental Surfaces ◽  
Environmental Surface ◽  
Aoac Method ◽  
Aoac Official ◽  
A Minor ◽  
And Control ◽  
Comparative Validation

Abstract The Visual Immunoprecipitate (VIP®) for the Detection of Listeria in Foods and Environmental Surfaces, AOAC Official Method 997.03, has been modified to change the color of the test and control lines of the device. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Two food matrixes and one environmental surface were analyzed. In total, there were valid results from 100 samples and controls. Results showed that the modified VIP Gold® for Listeria spp. is equivalent to the reference culture methods for the detection of Listeria.

scholarly journals Validation Study to Demonstrate the Equivalence of a Minor Modification of Assurance Enzyme Immunoassay Method, Official Method 996.14, for Detection of Listeria in Foods and Environmental Surfaces to the Reference Culture Methods

2008 ◽  
Vol 91 (1) ◽  
pp. 159-163
Author(s):  
Philip T Feldsine ◽  
David E Kerr ◽  
Andrew H Lienau
Keyword(s):  
Enzyme Immunoassay ◽  
Conjugate Addition ◽  
Official Method ◽  
Comparison Study ◽  
Culture Methods ◽  
Environmental Surfaces ◽  
Environmental Surface ◽  
Aoac Official ◽  
A Minor ◽  
Aoac Official Method

Abstract The Assurance Enzyme Immunoassay (EIA) for the Detection of Listeria in Foods and Environmental Surfaces, AOAC Official Method 996.14, has been modified to combine the separate antibody and conjugate addition steps into one. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture methods. Two food matrixes and one environmental surface were analyzed. In total, there were valid results from 145 samples and controls. Results showed that the Assurance EIA for Listeria spp. is equivalent to the reference culture methods for the detection of Listeria.

scholarly journals Visual Immunoprecipitate Assay Eight Hour Method for Detection of Enterohemorrhagic Escherichia coli O157:H7 in Raw and Cooked Beef (Modification of AOAC Official Method 996.09): Collaborative Study

2002 ◽  
Vol 85 (5) ◽  
pp. 1029-1036 ◽  
Author(s):  
Philip T Feldsine ◽  
David E Kerr ◽  
Stephanie C Leung ◽  
Andrew H Lienau ◽  
Ruth F Moser ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Collaborative Study ◽  
Culture Method ◽  
Enterohemorrhagic Escherichia Coli ◽  
Official Method ◽  
Escherichia Coli O157 ◽  
Culture Methods ◽  
Aoac Official ◽  
Inspection Service ◽  
Aoac Official Method

Abstract AOAC Official Method 996.09, Visual Immunoprecipitate Assay (VIP®) for Escherichia coli O157:H7, was modified to incorporate a new enrichment protocol using BioControl EHEC8™ medium for testing raw and cooked beef. Foods were tested by VIP assay and the U.S. Department of Agriculture/Food Safety and Inspection Service (USDA/FSIS) enrichment procedure and the FDA Bacteriological Analytical Manual (BAM) isolation and confirmation techniques. A total of 15 collaborators participated. Raw and cooked ground beef were inoculated with E. coli O157:H7 at 2 different levels: a high level, where predominantly positive results were expect d, and a low level where fractional recovery was anticipated. Collaborators tested 396 test portions and controls by both methods, for a total of 792 test portions. Of the 396 paired test portions, 75 were positive and 230 were negative by both the VIP and culture methods. Eleven test portions were presumptively positive by VIP and could not be confirmed culturally; 32 were negative by VIP, but confirmed positive by culture; and 65 were negative by the culture method, but confirmed positive by the VIP method. There was no statistical difference between results obtained with the VIP for EHEC 8 h method and the culture method except for cooked beef, where the VIP had significantly higher recovery for one inoculation level.

ComparativeValidation Study To Demonstrate the Equivalence of a Minor Modification to AOAC Official Method 2005.04 Assurance GDS® E. coli O157:H7 Method to the Reference Culture Method: 375 Gram Sample Size

2013 ◽  
Vol 96 (4) ◽  
pp. 776-780
Author(s):  
Philip T Feldsine ◽  
Megan Montgomery-Fullerton ◽  
Nerie Roa ◽  
Mandeep Kaur ◽  
Andrew H Lienau ◽  
...  
Keyword(s):  
Sample Size ◽  
Culture Method ◽  
Probability Of Detection ◽  
Official Method ◽  
Culture Methods ◽  
E Coli ◽  
Aoac Official ◽  
High Level ◽  
A Minor ◽  
Leaf Lettuce

Abstract The Assurance GDS® Escherichia coli (E. coli) O157:H7, AOAC Official MethodSM 2005.04, has been modified to include a larger sample size of 375 g. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Ninety samples and controls, representing three foods, were analyzed. Results show no statistically detectable difference between the Assurance GDS E. coli O157:H7 assay and the reference culture methods for the detection of E. coli O157:H7, other than the low level of inoculation for leaf lettuce, for which the GDS gave noticeably higher recovery [difference in probability of detection between candidate methods (dPODc = +0.45)]. There were also suggestions of moderate differences (dPODc = +0.15 to +0.20) for ground beef and the high level of leaf lettuce, but the study size was too small to detect differences of this size. Results showed that the Assurance GDS E. coli O157:H7 method is equivalent to reference culture methods for the detection of E. coli O157:H7.

Allyl Isothiocyanate in Mustard Seed

1970 ◽  
Vol 53 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Donald L Andersen
Keyword(s):  
Allyl Isothiocyanate ◽  
Mustard Seed ◽  
Official Method ◽  
Average Recovery ◽  
Aoac Method ◽  
Aoac Official ◽  
Mustard Seeds ◽  
Column Preparation ◽  
Aoac Official Method

Abstract A new GLC method for the determination of allyl isothiocyanate in mustard seed was compared to a method of the Midwest Research Institute and to a combination of the AOAC official method and the proposed method. Twelve collaborators compared the AOAC method and the GLC method, using whole mustard seeds. Each collaborator assayed three seed portions by both methods. The range, standard deviation, and coefficient of variation are less for each seed portion by the proposed than by the official method. The average recovery value of allyl isothiocyanate in the prepared standard solutions is lower, using the proposed GLC procedure, but seed assay values are significantly and consistently higher for each seed portion when compared with the results for the AOAC method. Reports from the collaborators also indicate that the proposed method is rugged, as the GLC column preparation was subjected to many changes. It is recommended that the GLC method be adopted as official first action.

scholarly journals Assurance® Enzyme Immunoassay Eight Hour Method for Detection of Enterohemorrhagic Escherichia coli O157:H7 in Raw and Cooked Beef (Modification of AOAC Official Method 996.10): Collaborative Study

2002 ◽  
Vol 85 (5) ◽  
pp. 1037-1044 ◽  
Author(s):  
Philip T Feldsine ◽  
David E Kerr ◽  
Stephanie C Leung ◽  
Andrew H Lienau ◽  
Stephanie M Miller ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enzyme Immunoassay ◽  
Collaborative Study ◽  
Ground Beef ◽  
Culture Method ◽  
Enterohemorrhagic Escherichia Coli ◽  
Official Method ◽  
Escherichia Coli O157 ◽  
Aoac Official ◽  
Aoac Official Method

Abstract AOAC Official Method 996.10, Assurance® Enzyme Immunoassay (EIA) for Escherichia coli O157:H7 (EHEC), was modified to incorporate a new enrichment protocol using BioControl EHEC8™ medium for testing raw and cooked beef. Foods were tested by EIA and the U.S. Department of Agriculture/Food Safety and Inspection Service (USDA/FSIS) enrichment conditions and the FDA Bacteriological Analytical Manual (BAM) isolation and confirmation techniques. A total of 14 collaborators participated. Raw and cooked ground beef were inoculated with E. coli O157:H7 at 2 different levels: a high level where predominantly positive results were expected, and a low level where fractional recovery was anticipated. Collaborators tested 378 test portions and controls by both the 8 h EIA and the USDA/FSIS enrichment methods, for a total of 756 test portions. Of the 378 paired test portions, 75 were positive and 212 were negative by both methods. Thirteen test portions were presumptively positive by EIA and could not be confirmed culturally; 30 were negative by EIA, but confirmed positive by culture; and 65 were negative by the culture method, but confirmed positive by the EIA method. There was no statistical difference between results obtained with the Assurance EIA for EHEC 8 h method and the culture method for raw ground beef. The Assurance EIA had a significantly higher recovery for cooked beef.

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