Evaluation of Transgenic Sweet Corn Hybrids Expressing CryIA(b) Toxin for Resistance to Corn Earworm and Fall Armyworm (Lepidoptera: Noctuidae)

1999 ◽  
Vol 92 (1) ◽  
pp. 246-252 ◽  
Author(s):  
R. E. Lynch ◽  
B. R. Wiseman ◽  
D. Plaisted ◽  
D. Warnick
Keyword(s):  
Sweet Corn ◽  
Fall Armyworm ◽  
Corn Earworm ◽  
Corn Hybrids ◽  
Lepidoptera Noctuidae

Evaluation of Bt Transgenic Sweet Corn Hybrids for Resistance to Corn Earworm and Fall Armyworm (Lepidoptera: Noctuidae) using a Meridic Diet Bioassay2

1999 ◽  
Vol 34 (4) ◽  
pp. 415-425 ◽  
Author(s):  
B. R. Wiseman ◽  
R. E. Lynch ◽  
D. Plaisted ◽  
D. Warnick
Keyword(s):  
Spodoptera Frugiperda ◽  
Helicoverpa Zea ◽  
Sweet Corn ◽  
Fall Armyworm ◽  
Corn Earworm ◽  
Laboratory Bioassay ◽  
Corn Hybrids ◽  
Pinto Bean ◽  
Highly Correlated ◽  
Lepidoptera Noctuidae

A laboratory bioassay was used to evaluate Bt transgenic sweet corn hybrids for resistance against the corn earworm, Helicoverpa zea (Boddie), and the fall armyworm, Spodoptera frugiperda (J. E. Smith). Whorl leaves, silks, and kernels, either fresh or oven-dried and ground with a mill, were incorporated into a dilute pinto bean diet and bioassayed against neonate, 3-, or 6-day-old larvae. Regardless of age of the larvae, results with the diet bioassay using fresh silks, oven-dried silks or fresh kernels were highly correlated with those for the fresh silk bioassay. Differences in susceptibility between insect species to the CrylA(b) toxin produced in the transgenic plants were also readily discernable using the diet bioassay. Based on results of the bioassays, Novartis sweet corn hybrids containing a crylA(b) gene gene for δ-endotoxin production were very highly resistant to leaf, silk and kernel feeding by the corn earworm and highly resistant to leaf and silk feeding by the fall armyworm.

Management of Corn Earworm and Fall Armyworm (Lepidoptera: Noctuidae) Injury on a Sweet Corn Hybrid Expressing a cryIA(b) Gene

1999 ◽  
Vol 92 (5) ◽  
pp. 1217-1222 ◽  
Author(s):  
R. E. Lynch ◽  
B. R. Wiseman ◽  
H. R. Sumner ◽  
D. Plaisted ◽  
D. Warnick
Keyword(s):  
Sweet Corn ◽  
Fall Armyworm ◽  
Corn Earworm ◽  
Lepidoptera Noctuidae ◽  
Corn Hybrid

scholarly journals Evaluation of Efficacy of Selected Conventional and Experimental Insecticides and Bt Hybrids for Control of Corn Earworm and European Corn Borer on Sweet Corn, 1997

1998 ◽  
Vol 23 (1) ◽  
pp. 99-100
Author(s):  
J. T. Shaw ◽  
R. Weinzierl ◽  
J. W. Finger
Keyword(s):  
European Corn Borer ◽  
Sweet Corn ◽  
Corn Earworm ◽  
Compressed Air ◽  
Hollow Cone ◽  
Corn Hybrids ◽  
Corn Borer ◽  
Chemical Treatments ◽  
Untreated Check ◽  
High Clearance

Abstract The tests were arranged in a RCB design with four replications. Each plot consisted of four rows, each being 30 inches wide and 50 ft long. Thirty-foot-wide alleys were established between the four replications. Insecticides were applied to the middle two rows of each plot, leaving two untreated rows between adjoining plots. Twelve insecticide treatments were compared with two Br-sweet corn hybrids (Heritage Bt and Bonus Bt) and their non-Bt isolines to two untreated controls. The 12 chemical treatments and the two untreated checks were planted with the Silver King variety sweet corn. Two untreated check plots were included in each replication and were averaged for the ANOVA. Treatments were applied very four to five days beginning on 8 Aug at 8% silk. All insecticide treatments were applied with a modified John Deere 6000 high-clearance vehicle (HCV) with a rear-mounted boom. Six Conejet (TX VS-8) hollow-cone nozzles (three per row) were calibrated to deliver 30.1 gpa at 40 psi and a speed of 2.5 mph, utilizing a compressed air system. Four nozzles (two per row) were attached to drops and directed the spray towards the ear zone area, a third nozzle (one per row) was mounted directly over the row with the spray being directed into the whorl (tassel) area.

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