Efficacy of Ethanolic plant extracts of Zingiber officinale, Raphanus sativus, Rosa indica and Aloe vera against Heterotermes indicola

Present study was performed to evaluate the anti-termitic potential of four common plant species of Zingiber officinale, Raphanus sativus, Rosa indica and Aloe vera. During laboratory bioassay, the biological activity of ethanolic extracts of these plants was evaluated. The ethanol extract of Z. officinale caused highest mortality (100%) while least mortality (68%) was observed in R. indica extract. The LT50 for Z. officinale were 24.34, 40.40 and 64.7 h for concentrations of 10%, 5% and 3% respectively. Present findings suggested that these plant extracts can provide environmental friendly management of H. indicola. In A. vera, also resulted in significant mortality against H. indicola i.e. 94%, 64% and 44% on exposure of 10%, 5% and 3% concentraions. While LT50 were 34.66, 65.59, and 100.1 hours against 10%, 5% and 3% concentrations. In R. sativus, mortality of H. indicola was 76%, 50% and 30% while LT50 against were 59.10, 86.20, and 127.3 h, against concentration of 10%, 5% and 3%. Least mortality was observed in R. indica i.e. 68%, 44% and 30% while LT50 were 64.17, 94.58 and 125.5 h at 10%, 5% and 3% respectively. This study revealed that the extracts of Z. officinale, A.vera and R. Sativus have a potential to be used for termite control especially Heterotermes indicola to minimize the damage. Copyright(c) The Author

Effect of Endectocides and Antibiotic Dung Poisoning on Mortality of Dung Beetle Species

We explored the effect of endectocides (deltamethrin and trichlorophenol) and antibiotics (enrofloxacin and oxytetracycline) on dung beetle species, 0nthophagus gazella and 0nitis excavatus under laboratory conditions. 0. gazella and 0. excavatus were collected from pastures and crop lands of Jhelum, Punjab, Pakistan. The selected endectocides and antibiotics were applied on dung pats placed over soil @ 0.25, 0.50, 0.75 and 1 ppm and kept in glass containers of 2' x 2' x 1.5' size. The laboratory bioassay was carried out at 28 ± 2 °C temperature and 65 ± 5 % RH with 12:12 of light to darkness ratio. Our results demonstrated that deltamethrin, trichlorophenol and oxytetracycline were toxic to both species, 0. gazella and 0. excavatus after two weeks of the exposure. Comparatively higher dung beetle mortality was recorded in both deltamethrin and trichlorophenol when applied @ 1 ppm as compared to 0.25, 0.50 and 0.75 ppm concentrations in 0. gazella and 0. excavatus showed significantly higher mortality against oxytetracycline (53.3 % and 93.3 %, respectively), trichlorophenol (80 % and 94 %, respectively) and deltamethrin (88% and 76 %, respectively) at 1 ppm concentrations, whereas no mortality was recorded in all concentrations of enrofloxacin. 0. gazella and 0. excavatus responded negatively to the presence of different concentrations of veterinary parasiticides. In addition to the hazardous effect of 0. gazella and 0. excavatus, the study reports that these dung beetle species can be exploited as bio-indicators for environmental assessment in ecosystem studies.

Spray and Pour-On Acaricides Killed Tennessee (United States) Field-Collected Haemaphysalis longicornis Nymphs (Acari: Ixodidae) in Laboratory Bioassays

Haemaphysalis longicornis Neumann (Asian longhorned tick) is an exotic and invasive tick species presenting a health and economic threat to the United States (U.S.) cattle industry due to its ability to transmit pathogens and infest hosts in large numbers. The objective of this study was to evaluate available products at causing H. longicornis mortality in a laboratory bioassay. The efficacy of products was evaluated at label rates using H. longicornis nymphs collected from a cattle farm in eastern Tennessee in two different bioassays (spray or dip) against untreated controls. After exposure, ticks were transferred to clean petri dishes and checked for mortality at 0, 1, 2, 3, 4, 21, 24, and 48 h post exposure. No mortality occurred in the untreated controls, whereas all treated ticks were dead within 24 h of exposure (P < 0.0001). These findings support the hypothesis that currently available spray and pour-on products are effective at causing H. longicornis mortality. We conclude that these acaricides can be used as a component to prevent H. longicornis dispersal and for control in the U.S.

Using Citronella to Protect Bees (honeybee Apis mellifera L.) from certain Insecticides and Their Nano Formulations

Experiments were performed investigating citronella (Cymbopogon winterianus Jowitt) as a repellent to honeybee Apis mellifera (L.) (Hymenoptera: Apidae) in Egypt, it was conducted in laboratory in the Department of Entomology and Pesticides Science, Faculty of Agriculture, Cairo University, to check long-term survival of honeybee when exposed to different nano insecticides alone or combined with citronella at the same examination box for each. In this study, we used a modeling approach regarding survival data of caged worker bees under chronic exposure to four insecticides (Chloropyrophos, Nano-chloropyrophos Imidacloprid, Nano-Imidacloprid) each of them was supplemented in a box alone and in combination with citronella. Having three replicates and five concentrations (100, 200, 300, 400 and 500 ppm). Laboratory bioassay of these insecticides showed that chloropyrophos and nano chloropyrophos were the most toxic at their high dose (500 ppm) with LT50 of 120.98 and 122.02 followed by 132.14 and 136.5 minutes for Imidacloprid and Nano-Imidacloprid, respectively. No consumption occurred by bees to mixed sugar syrup with insecticides in all treatments when citronella was added. These data highly recommended that adding citronella is very effective when nicotinoid pesticides are used to longevity honeybee life and keep bee safe.