Effects of Diet, Ginger Root Oil, and Elevation on the Mating Competitiveness of Male Mediterranean Fruit Flies (Diptera: Tephritidae) from a Mass-Reared, Genetic Sexing Strain in Guatemala

AbstractThe sexual performance of Anastrepha ludens males of the Tapachula-7 genetic sexing strain, produced via selection based on mating success, was compared with that of males produced without selection in competition with wild males. Mating competition, development time, survival, mass-rearing quality parameters and pheromone production were compared. The results showed that selection based on mating competitiveness significantly improved the sexual performance of offspring. Development time, survival of larvae, pupae and adults, and weights of larvae and pupae increased with each selection cycle. Differences in the relative quantity of the pheromone compounds (Z)-3-nonenol and anastrephin were observed when comparing the parental males with the F4 and wild males. The implications of this colony management method on the sterile insect technique are discussed.

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Male lures and male mating competitiveness in a genetic sexing strain of the melon fly (Diptera: Tephritidae)

Journal of Asia-Pacific Entomology ◽

10.1016/j.aspen.2019.01.014 ◽

2019 ◽

Vol 22 (2) ◽

pp. 393-397 ◽

Cited By ~ 2

Author(s):

Todd E. Shelly

Keyword(s):

Male Mating ◽

Genetic Sexing ◽

Melon Fly ◽

Mating Competitiveness ◽

Genetic Sexing Strain ◽

Male Lures

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Mating Competitiveness of Irradiated Male Stable Flies, Stomoxys Calcitrans (Diptera: Muscidae), from a Genetic Sexing Strain

Journal of Medical Entomology ◽

10.1093/jmedent/21.2.179 ◽

1984 ◽

Vol 21 (2) ◽

pp. 179-182 ◽

Cited By ~ 2

Author(s):

B.J. Smittle ◽

J.A. Seawright ◽

N.L. Willis

Keyword(s):

Stomoxys Calcitrans ◽

Genetic Sexing ◽

Stable Flies ◽

Mating Competitiveness ◽

Genetic Sexing Strain

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Determining the Sterilization Doses under Hypoxia for the Novel Black Pupae Genetic Sexing Strain of Anastrepha fraterculus (Diptera, Tephritidae)

Insects ◽

10.3390/insects12040308 ◽

2021 ◽

Vol 12 (4) ◽

pp. 308

Author(s):

Paloma Della Giustina ◽

Thiago Mastrangelo ◽

Sohel Ahmad ◽

Gabriel Mascarin ◽

Carlos Caceres

Keyword(s):

Fruit Fly ◽

South American ◽

Response Curves ◽

Genetic Sexing ◽

Egg Hatch ◽

Anastrepha Fraterculus ◽

Common Strategy ◽

Genetic Sexing Strain ◽

South American Fruit Fly ◽

First Time

A common strategy used to maintain sterile fly quality without sacrificing sterility is to irradiate the insects under an oxygen-reduced atmosphere. So far, sterilizing doses for the South American fruit fly Anastrepha fraterculus have only been determined under normoxia. Our study reports for the first time the dose-sterility response under hypoxia for two different A. fraterculus strains. The pupae were derived from a bisexual strain (a Brazilian-1 population) and a recently developed genetic sexing strain (GSS-89). Two hours prior to irradiation, pupae were transferred to sealed glass bottles and irradiated when oxygen concentration was below 3%. Four types of crosses with nonirradiated flies of the bisexual strain were set to assess sterility for each radiation dose. For males from both strains, Weibull dose–response curves between radiation doses and the proportion of egg hatch, egg-to-pupa recovery, and recovery of adults were determined. The GSS males revealed high sterility/mortality levels compared to males from the bisexual strain at doses < 40 Gy, but a dose of 74 Gy reduced egg hatch by 99% regardless of the male strain and was considered the sterilizing dose. The fertility of irradiated females was severely affected even at low doses under hypoxia.

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Pupation Substrate Type and Volume Affect Pupation, Quality Parameters and Production Costs of a Reproductive Colony of Ceratitis capitata (Diptera: Tephritidae) VIENNA 8 Genetic Sexing Strain

Insects ◽

10.3390/insects12040337 ◽

2021 ◽

Vol 12 (4) ◽

pp. 337

Author(s):

Carlos Pascacio-Villafán ◽

Luis Quintero-Fong ◽

Larissa Guillén ◽

José Pedro Rivera-Ciprian ◽

Reynaldo Aguilar ◽

...

Keyword(s):

Wheat Bran ◽

Ceratitis Capitata ◽

Mass Rearing ◽

Physicochemical Characteristics ◽

Production Costs ◽

Cellulose I ◽

Coconut Fiber ◽

Genetic Sexing ◽

Recycled Paper ◽

Genetic Sexing Strain

Adequate pupation substrates and substrate volume are critical factors in the mass-rearing of insects for Sterile Insect Technique (SIT) applications. To identify an ideal pupation substrate for a reproductive colony of Ceratitis capitata (Wiedemann) VIENNA 8 genetic sexing strain, we first examined pupation in cellulose from recycled paper (cellulose I), sawdust, fine wheat bran, vermiculite and coconut fiber using a volume of 2.5–12.5 mL of substrate for each 5 mL volume of fly larvae. We found a positive relationship between substrate volume and pupation, with cellulose I generating the highest proportions of pupation and coconut fiber the lowest. Higher proportions of female flies (white pupae) pupated in sawdust. The proportion of female fliers increased as substrate volume rose in sawdust and coconut fiber, whereas it decreased in vermiculite and cellulose. In a second experiment, we tested three types of cellulose differing in physicochemical characteristics (celluloses I, II and III), sawdust, and fine wheat bran using a substrate:larvae ratio of 1:1. The three types of cellulose produced the highest pupation levels. The highest proportions of female fliers were observed in sawdust, and cellulose types III and II. Cellulose III and sawdust at relatively low volumes were more cost-effective to produce one million pupae than other substrates, including fine wheat bran used in a mass-rearing facility in Mexico.

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