The phylogenetic relationships amongst 29 species of
Carlia and Lygisaurus were
estimated using a 726-base-pair segment of the protein-coding mitochondrial
ND4 gene. Results do not support the recent resurrection of the genus
Lygisaurus. Although most
Lygisaurus species formed a single clade, this clade is
nested within Carlia and includes
Carlia parrhasius. Due to this new molecular evidence,
and the paucity of diagnostic morphological characters separating the genera,
Lygisaurus de Vis 1884 is re-synonymised with
Carlia Gray 1845. Our analysis is also inconsistent with
a previous suggestion that Lygisaurus timlowi should be
removed to Menetia, a genus that is distantly related
relative to outgroups used here. Intraspecific variation in
Carlia is, in several instances, greater than
interspecific distance. The most strikingly divergent lineages are found
within C. rubrigularis, which appears to be
paraphyletic, with southern populations more closely related to
C. rhomboidalis than to northern populations of
C. rubrigularis. The two
C. rubrigularis–C. rhomboidalis lineages form part
of a major polytomy at an intermediate level of divergence. Lack of resolution
at this level, however, does not appear to be due to saturation or loss of
phylogenetic signal. Rather, the polytomy probably reflects a period of
relatively rapid diversification that occurred sometime during the Miocene.