Chromosome Banding Patterns of Rat Fibrosarcomas Induced by In Vitro Transformation of Embryo Cells or In Vivo Injection of Rats by 7,12-Dimethylbenz[a] anthracene

Chick embryo fibroblasts infected in vitro with Rous sarcoma virus have properties similar to tumor cells when injected into virus-immune chickens. When such virus-transformed fibroblasts are injected into normal chickens, they apparently participate in the production of tumors independent of their release of virus and are thus apparently malignant in vivo.

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Effectiveness of exogenous DNA transfer to chicken embryo cells in vitro and in vivo using retroviral vectors

Russian Agricultural Sciences ◽

10.3103/s1068367407030135 ◽

2007 ◽

Vol 33 (3) ◽

pp. 180-182 ◽

Cited By ~ 1

Author(s):

N. A. Volkova ◽

A. O. Tulyakova ◽

L. A. Volkova ◽

N. A. Zinov’eva ◽

L. K. Ernst ◽

...

Keyword(s):

Chicken Embryo ◽

Retroviral Vectors ◽

Dna Transfer ◽

Exogenous Dna ◽

Embryo Cells

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Methylation and expression of the Myo D1 determination gene

Philosophical Transactions of the Royal Society of London Series B Biological Sciences ◽

10.1098/rstb.1990.0011 ◽

1990 ◽

Vol 326 (1235) ◽

pp. 277-284 ◽

Cited By ~ 22

Keyword(s):

De Novo ◽

Cell Types ◽

Model System ◽

Parental Line ◽

Molecular Control ◽

Embryo Cells ◽

End Stage ◽

Derivatives Of

Mouse embryo cells induced to differentiate with the demethylating agent 5- azacytidine represent an excellent model system to investigate the molecular control of development. Clonal derivatives of 10T1/2 cells that have become determined to the myogenic or adipogenic lineages can be isolated from the multipotential parental line after drug treatment. These determined derivatives can be cultured indefinitely and will differentiate into end-stage phenotypes on appropriate stimulation. A gene called Myo D1, recently isolated from such a myoblast line, will confer myogenesis when expressed in 10T1/2 or other cell types (Davis et al. 1987). The cDNA for Myo D1 contains a large number of CpG sequences and the gene is relatively methylated in 10T1/2 cells and an adipocyte derivative, but is demethylated in myogenic derivatives. Myo D1 may therefore be subject to methylation control in vitro . On the other hand, preliminary observations suggest that Myo D1 is not methylated at CCGG sites in vivo so that a de novo methylation event may have occurred in vitro . These observations may have significance in the establishment of immortal cell lines and tumours.

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In vitro transformation of mouse embryo cells by SV 40 DNA

Okayama Igakkai Zasshi (Journal of Okayama Medical Association) ◽

10.4044/joma1947.83.7-8_267 ◽

1971 ◽

Vol 83 (7-8) ◽

pp. 267-271

Author(s):

Shinichiro YAMAMOTO ◽

Kuniyuki EGUSA ◽

Takuzo ODA

Keyword(s):

Mouse Embryo ◽

In Vitro Transformation ◽

Embryo Cells

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Evidence of Genomic Instability inCampylobacter jejuni Isolated from Poultry

Applied and Environmental Microbiology ◽

10.1128/aem.64.5.1816-1821.1998 ◽

1998 ◽

Vol 64 (5) ◽

pp. 1816-1821 ◽

Cited By ~ 107

Author(s):

Trudy M. Wassenaar ◽

Barbara Geilhausen ◽

Diane G. Newell

Keyword(s):

Chromosomal Dna ◽

Unrelated Control ◽

Banding Patterns ◽

Phage Types ◽

Single Colony ◽

Pcr Products ◽

Colonization Model ◽

Phenotypic Methods

ABSTRACT Poultry isolates of Campylobacter jejuni derived from a survey of meat processing batches were genotyped by pulsed-field gel electrophoresis (PFGE) of chromosomal DNA to establish the clonal relationships between single-colony isolates. In the majority of batches studied, one or two genotype patterns predominated. However, in one batch (batch A), 21 single-colony isolates gave 14 different PFGE genotypes. The banding patterns obtained with SmaI were sufficiently different to distinguish between genotypes, although the patterns also produced many common bands. The question of whether these isolates represented different clones or had a common clonal ancestry was addressed by additional genotypic and phenotypic methods. Restriction length polymorphism of PCR products obtained from the flagellin genes showed an identical flagellin genotype for all of these isolates. In contrast, unrelated control isolates resulted in different flagellin genotypes. Moreover, all 14 different PFGE genotypes of batch A had identical Penner serotypes and identical or similar biotypes and phage types. It was concluded that the isolates were of clonal origin and that the diversity in the PFGE banding patterns had most likely originated from genomic rearrangements. However, the PFGE genotypes were shown to be stable upon subculturing in vitro and after in vivo passage in chickens, and natural transformation between isogenic mutants carrying antibiotic markers did not occur in vivo in a chick colonization model. The possible mechanisms for the hypothesized genomic recombinations and the conditions that allow, induce, or select for such events are discussed.

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