Characterization of Mouse Mammary Tumor Viruses From Primary Tumor Cell Cultures. I. Immunologic and Structural Studies 2

1976 ◽  
Vol 56 (1) ◽  
pp. 111-117 ◽  
Author(s):  
P. C. Kimball ◽  
M. Boehm-Truitt ◽  
G. Schochetman ◽  
J. Schlom
Keyword(s):  
Tumor Cell ◽  
Cell Cultures ◽  
Mammary Tumor ◽  
Primary Tumor ◽  
Structural Studies ◽  
Primary Tumor Cell ◽  
Tumor Viruses ◽  
Mouse Mammary Tumor

Electron Immunochemical Labeling of Ultrathin Sections of Murine Tumor Viruses and Cell Cultures using the Unlabeled Antibody Enzyme Technique

1976 ◽  
Vol 34 ◽  
pp. 266-267
Author(s):  
W. J. Hamilton
Keyword(s):  
Mammary Tumor ◽  
Leukemia Virus ◽  
Parallel Methods ◽  
Tumor Viruses ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
Antibody Conjugates ◽  
Rauscher Leukemia ◽  
Antigen Localization ◽  
Unlabeled Antibody

The study of RNA tumor viruses has been greatly facilitated by the use of immunochemical tagging methods. In the past these methods have been constrained to antibody conjugates with ferritin or peroxidase. In order to avoid the disadvantages of using conjugated antisera, investigators have applied the unlabeled antibody enzyme method of Sternberger to mammary tumor derived mouse cells prior to embedding for electron microscopy. The current study has successfully applied the Sternberger method to virusproducing cells and purified virus pellets after epoxy-embedding and ultrathin sectioning. The results demonstrate the distinct advantages of this “post-embedding” method for viral antigen localization.Purified Rauscher leukemia virus (RLV) and mouse mammary tumor virus (MMTV) were pelleted, fixed in buffered 2% paraformaldehyde and washed thoroughly. These were dehydrated in acetone, infiltrated and embedded in Spurr resin according to common procedures. A tumor derived cell line, Mm5mt, producing MMTV was embedded by parallel methods.

Isolation, Identification, and Assay of [3H]-Porfiromycin Adducts of EMT6 Mouse Mammary Tumor Cell DNA: Effects of Hypoxia and Dicumarol on Adduct Patterns

1991 ◽  
Vol 3 (7) ◽  
pp. 213-223 ◽  
Author(s):  
Maria Tomasz ◽  
Christine S. Hughes ◽  
Dondapati Chowdary ◽  
Susan Riley Keyes ◽  
Roselyn Lipman ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Mammary Tumor ◽  
Mammary Tumor Cell ◽  
Mouse Mammary Tumor ◽  
Mouse Mammary Tumor Cell

Ecotropic murine leukemia viruses and exogenous mouse mammary tumor viruses are not essential for pristane-induced lupus

2003 ◽  
Vol 48 (10) ◽  
pp. 2990-2992 ◽  
Author(s):  
Jun Akaogi ◽  
Dina C. Nacionales ◽  
Yoshiki Kuroda ◽  
Westley H. Reeves ◽  
Minoru Satoh
Keyword(s):  
Mammary Tumor ◽  
Tumor Viruses ◽  
Mouse Mammary Tumor ◽  
Leukemia Viruses ◽  
Murine Leukemia
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