Comparative Chromosome Study of Normal Mammary Tissue, Carcinogen-Induced Mammary Tumors, and Hyperplastic Alveolar Nodules in the Rat: Brief Communication 2

1977 ◽  
Vol 59 (3) ◽  
pp. 1007-1012 ◽  
Author(s):  
Yoshikazu Takahashi ◽  
Dilip K. Sinha ◽  
Thomas L. Dao
Keyword(s):  
Mammary Tumors ◽  
Chromosome Study ◽  
Mammary Tissue ◽  
Normal Mammary Tissue

scholarly journals FAMILIAL MAMMARY TUMORS IN THE RABBIT

1939 ◽  
Vol 70 (2) ◽  
pp. 167-184 ◽  
Author(s):  
Harry S. N. Greene
Keyword(s):  
Mammary Tumors ◽  
Mammary Tissue ◽  
Tumor Type ◽  
Natural Classification ◽  
Tumor Bearing ◽  
Clinically Normal ◽  
Experimental Induction ◽  
Papillary Structure ◽  
Natural Counterpart

The clinical and pathological course of 25 mammary tumors in rabbits has been described. The antecedent breast history and morphology of the growths allowed a natural classification into two distinct types, one of which was distinguished by a preexisting cystic mastitis and a papillary structure, while the other originated in clinically normal mammary tissue and was characterized by an adenomatous structure. The two types of neoplasia occurred almost exclusively in two family groups and heredity played a fundamental rôle both in the occurrence of the tumors and in the determination of tumor type. Endocrine changes, comparable with those found in animals after long continued administration of estrogenic substances, occurred in tumor-bearing rabbits and it was inferred that the spontaneous growths represented a natural counterpart of the experimental induction of neoplasia with estrone.

scholarly journals Localization of alpha-casein gene transcription in sections of epoxy resin-embedded mouse mammary tissues by in situ hybridization.

1988 ◽  
Vol 36 (12) ◽  
pp. 1503-1510 ◽  
Author(s):  
D S Liscia ◽  
P J Doherty ◽  
G H Smith
Keyword(s):  
In Situ Hybridization ◽  
Epoxy Resin ◽  
Specific Activity ◽  
Mammary Tumors ◽  
Image Resolution ◽  
Hybridization Signal ◽  
Mammary Tissue ◽  
Positive Hybridization ◽  
Mammary Tissues

The objective of our study was to evaluate the suitability of aldehyde-fixed, epoxy resin-embedded tissue for efficient and reproducible detection of casein mRNA in mouse mammary tissue by in situ hybridization. We used mouse alpha-casein-specific, 35S-labeled riboprobes generated from a Gemini-3 vector. Both complementary (anti-sense) and homologous (sense) RNA probes were utilized in our study (specific activity ranged from 5-7 x 10(8) cpm/micrograms). We tested the stability of newly synthesized [3H]-uridine-labeled RNA in tissue sections subjected to epoxy plastic solvents and found that no detectable loss of label occurred during preparation of semi-thin (1-2 micron) plastic sections for situ hybridization. In addition, it was possible to detect alpha-casein mRNA in deplasticized sections of mammary gland tissue taken from normal, pregnant, or lactating mice, pre-neoplastic mammary alveolar hyperplasias, explant cultures, and mammary tumors. A positive hybridization signal was consistently obtained in sections of mammary tissues where the estimated average copy number for total casein mRNA was greater than or equal to 250/cell. In mammary tumors, where the estimated casein mRNA content was much lower (less than 5/cell), our positive hybridization signal occurred in regions of the tumor that, in consecutive sections, stained positive for casein by immunoperoxidase. After formaldehyde-glutaraldehyde fixation, loss of hybridizable RNA from epoxy-embedded tissues and sections appears to be minimal. Image resolution was greatly enhanced over frozen or paraffin sections of mammary tissue. Non-specific binding of the radioactive probes was very low. Protease treatment of the sections was not necessary for detection of hybridizable signal.

Parvovirus H-1 inhibits growth of short-term tumor-derived but not normal mammary tissue cultures

1993 ◽  
Vol 55 (4) ◽  
pp. 672-677 ◽  
Author(s):  
C. Van Pachterbeke ◽  
M. Tuynder ◽  
J. Rommelaere ◽  
J. P. Cosyn ◽  
L. Lespagnard ◽  
...  
Keyword(s):  
Mammary Tissue ◽  
Tissue Cultures ◽  
Short Term ◽  
Normal Mammary Tissue

scholarly journals THE RELATION OF COAT COLOR TO THE SPONTANEOUS INCIDENCE OF MAMMARY TUMORS IN MICE

1934 ◽  
Vol 59 (2) ◽  
pp. 229-250 ◽  
Author(s):  
C. C. Little
Keyword(s):  
Lower Incidence ◽  
Mammary Cancer ◽  
Coat Color ◽  
Virgin Female ◽  
Mammary Tumors ◽  
Color Difference ◽  
Tumor Formation ◽  
Mammary Tissue ◽  
Biological Phenomenon ◽  
Pass Through

1. The material included in this paper consists of F1 and F2 virgin female mice derived from a cross between a strain high in mammary cancer incidence (dilute brown) and one relatively low in incidence of mammary cancer but relatively high in the incidence of various internal tumors (yellow). 2. In the F1 and F2 hybrid generations the yellow animals have a significantly lower incidence of mammary tumors than do the non-yellows. This is the first clear case of a difference in the incidence of spontaneous tumors in mice associated with a color difference. 3. Mammary tumors occur, however, significantly earlier in the yellow mice and are just as malignant as those appearing in the non-yellows. 4. The incidence of tumors other than mammary is not significantly different in the yellow and non-yellow hybrids. Such tumors, however, occur distinctly later in life than do the mammary tumors. This provides additional evidence that, in mice, mammary tumors cannot be considered to be the same biological phenomenon as are other types of tumor. 5. A study of the physiology of reproduction of yellow and non-yellow mice within the yellow stock suggests that the yellows pass through their reproductive cycle earlier than do the non-yellows. The duration of the cycle in the two forms is essentially equal. This fact would satisfactorily explain the earlier incidence of mammary tumors in yellow mice. 6. The lower incidence of mammary tumors in yellows as compared with non-yellows may be at least in part due to the same phenomenon. This would follow because the opportunity for mammary tissue in yellow mice of cancer age to be continuously affected by ovarian secretion would be less than in non-yellows. This would result in a higher percentage of yellows reaching an age at which stimuli from the ovary ceased before the mammary tissue had reached an age at which tumor formation is most frequent. 7. There is some evidence that, in this cross, dilute (dbdb) mice are less apt to form mammary tumors than are intensely pigmented animals. This point, however, needs further investigation before it can be considered to be established. 8. The facts recorded in this paper demonstrate that not all forms of tumor or all colors of mice can be lumped together in studying either the physiology or genetics of spontaneous tumor incidence.

scholarly journals Immunohistochemical Analysis of Na+/K+-ATPase and Bone Morphogenetic Protein-2 Expressions in Both Parenchymal and Stromal Cells from Benign and Malignant Canine Mammary Tumors

2021 ◽  
Author(s):  
ozlem ozmen
Keyword(s):  
Stromal Cells ◽  
Malignant Tumors ◽  
Breast Tumors ◽  
Mammary Tumors ◽  
Bone Morphogenetic Protein 2 ◽  
Mammary Tissue ◽  
Benign Tumors ◽  
Ion Pump ◽  
Human Breast ◽  
Canine Mammary Tumors

Abstract Canine mammary tumors are the most common type of dog tumor, and they are similar to human breast tumors. Na+/K+-ATPase is a common plasma membrane ion pump with important physiological and pathophysiological functions. In mammary tumors, the tumor microenvironment was composed of a heterogeneous population of tumor cells and nearby endogenous stromal cells. Bone morphogenetic proteins (BMPs) regulate fetal development, tissue homeostasis and differentiation, and a variety of cellular functions. The purpose of this study is to examine the immunohistochemical expression of Na+/K+-ATPase and BMP-2 in tumoral and stromal cells from benign and malignant canine mammary tumors. In this study, ten benign and ten malignant mammary tumors from the archives of the Department of Pathology were used, with five normal breast tissues serving as controls. The results of the revealed that tumors had higher levels of Na+/K+-ATPase and BMP-2 expressions than normal mammary tissue. While both markers were expressed negatively or mildly in benign tumors, they increased significantly in malignant tumors. Both Na+/K+-ATPase and BMP-2 are expressed by tumoral and stromal cells in canine mammary tumors. When compared to compared to BMP-2, Na+/K+-ATPase expression was found to be more severe. This study found that Na+/K+-ATPase and BMP-2 can be used as markers of malignancy in canine mammary tumors and that stromal cells also play an important role in tumor progression. These findings also indicated that Na+/K+-ATPase and BMP-2 may be used for early diagnosis or as a potential target for treatment of canine and human breast tumors in the future.

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