Can Animal and In Vitro Studies Give New, Relevant Answers to Questions Concerning Mammographic Screening for Human Breast Cancer?23

1978 ◽  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Mammographic Screening ◽  
In Vitro Studies ◽  
Human Breast

Assay of Gcdfp-15 by Elisa: An Available Method for in Vitro Studies of Functional Differentiation in Human Breast Cancer

1988 ◽  
Vol 74 (6) ◽  
pp. 669-674
Author(s):  
Françoise Revillion-Carette ◽  
Louis Hornez ◽  
Brigitte Vandewalle ◽  
Jean Lefebvre
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Culture Media ◽  
Functional Differentiation ◽  
In Vitro Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Breast ◽  
Breast Cyst Fluid ◽  
Breast Cyst

An enzyme-linked immunosorbent assay (ELISA) was applied to a light protein, isolated from human breast cyst fluid (BCF) termed « gross cystic disease fluid protein - 15 Kda » (GCDFP-15), a potential differentiation marker in in vitro human breast cancer studies. The detection limits of this procedure, performed in microtiter plates, were 0.5 to 250 ng/well corresponding to 10 ng/ml to 5 ng/ml of sample or antigen solution. Possible cross-reaction with various antigens, especially those found in culture media, were investigated. The correlation coefficient between enzymoassay and radioimmunoassay was 0.978. The results showed that quantification of GCDFP-15 by ELISA is a specific and highly sensitive method. This procedure may be of interest in in vitro studies on the functional differentiation of breast cancer cells.

scholarly journals Heme biosynthesis in human breast cancer—mimetic “in vitro” studies and some heme enzymic activity levels

1990 ◽  
Vol 22 (12) ◽  
pp. 1407-1411 ◽  
Author(s):  
Nora M. Navone ◽  
Cesar F. Polo ◽  
Alberto L. Frisardi ◽  
Nelida E. Andrade ◽  
alcira M. del C. Baille
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Enzymic Activity ◽  
Heme Biosynthesis ◽  
In Vitro Studies ◽  
Activity Levels ◽  
Human Breast
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