scholarly journals Emerging applications of the single cell gel electrophoresis (Comet) assay. I. Management of invasive transitional cell human bladder carcinoma. II. Fluorescent in situ hybridization Comets for the identification of damaged and repaired DNA sequences in individual cells

Mutagenesis ◽  
1998 ◽  
Vol 13 (1) ◽  
pp. 1-8 ◽  
Author(s):  
V. J. McKelvey-Martin ◽  
E. T.S. Ho ◽  
S. R. McKeown ◽  
S.R. Johnston ◽  
P. J.McCarthy ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Comet Assay ◽  
Dna Sequences ◽  
Bladder Carcinoma ◽  
Gel Electrophoresis ◽  
Fluorescent In Situ Hybridization ◽  
Transitional Cell ◽  
Human Bladder ◽  
Single Cell Gel Electrophoresis

Searching for telomeric sequences in two Allium species

Genome ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 640-643 ◽  
Author(s):  
N Cuñado ◽  
E Sánchez-Morán ◽  
J Barrios ◽  
J L Santos
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequences ◽  
Fluorescent In Situ Hybridization ◽  
Two Dimensional ◽  
Ltr Retrotransposons ◽  
Allium Species ◽  
Telomeric Sequences ◽  
Rdna Sequences ◽  
Dimensional Surface

Some Alliaceae species have no tandemly repeated TTTAGGG sequences. Instead, at the very end of their chromosomes, there are highly repetitive satellite and (or) rDNA sequences. These sequences apparently replace the canonical plant telomeric sequences in these species. A method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs), combined with fluorescent in situ hybridization, has revealed that telomeric chromatin is tightly condensed at the ends of SCs in plants and animals. Using this method, we have tested the organization and location of those sequences postulated to cap the chromosomes in two species of the genus Allium: A. cepa and A. altaicum. We have also extended this study to other putative telomere candidates, such as LTR (long terminal repeat) and non-LTR retrotransposons. None of the DNA sequences analyzed showed the characteristic telomeric organization at pachytene.Key words: fluorescent in situ hybridization, meiosis, repetitive DNA, Allium, synaptonemal complex.

scholarly journals Increasing the resolution of the comet assay using fluorescent in situ hybridization--a review

Mutagenesis ◽  
2009 ◽  
Vol 24 (5) ◽  
pp. 383-389 ◽  
Author(s):  
S. Shaposhnikov ◽  
E. Frengen ◽  
A. R. Collins
Keyword(s):  
In Situ Hybridization ◽  
Comet Assay ◽  
Fluorescent In Situ Hybridization

Analysis of bacterial community in membrane-separation bioreactors by fluorescent in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) techniques

2000 ◽  
Vol 41 (10-11) ◽  
pp. 259-268 ◽  
Author(s):  
B.S. Luxmy ◽  
F. Nakajima ◽  
Kazuo Yamamoto
Keyword(s):  
In Situ Hybridization ◽  
Bacterial Communities ◽  
Gel Electrophoresis ◽  
Fluorescent In Situ Hybridization ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Membrane Separation ◽  
Diversity Index ◽  
Ammonia Oxidizing Bacteria ◽  
Gradient Gel Electrophoresis

The bacterial communities of membrane-separation bioreactors (MBR) fed with raw sewage were analyzed by a pilot scale study. The community was analyzed by both Fluorescent in Situ Hybridization (FISH) and PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) techniques. Five rRNA targeted group specific oligonucleotide probes showed that the alpha- and beta- subclasses of proteobacteria were the most dominant groups among them. The identification of ammonia-oxidizing bacteria in MBR was confirmed by three probes: NEU, Nsv 443 and Nso 190. Mostly the ammonia-oxidizers were found in groups and present in the form of clusters or aggregates. The ratio of NEU/EUB was estimated by double hybridization and image analysis techniques as 6%. The Nitrobacter sp. was also identified inside the MBR with the help of a NIT3 probe and they were also found to be present in the form of a cluster. Usually the clusters formed by the Nitrobacter sp. were smaller than those of ammonia-oxidizing groups. After numerical analysis on the band pattern of DGGE, it was found that the MBR bacterial communities were different from that of conventional activated sludge (CAS) communities with dissimilarity indexes more than 0.6. The diversity of the microbial community was estimated by the Shannon-Weaver index of general diversity. It was found that the value of the diversity index for the CAS process was 1.61 while those for two MBR processes were 1.68 and 1.59.

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