scholarly journals Mutations in domain V of the 23S ribosomal RNA of Bacillus subtilis that inactivate its protein folding property in vitro

2002 ◽  
Vol 30 (5) ◽  
pp. 1278-1285 ◽  
Author(s):  
S. Chowdhury
Keyword(s):  
Protein Folding ◽  
Bacillus Subtilis ◽  
Ribosomal Rna ◽  
Domain V ◽  
23S Ribosomal Rna

scholarly journals A small sequence in domain v of the mitochondrial large ribosomal RNA restores Drosophila melanogaster pole cell determination in uv-irradiated embryos

2010 ◽  
Vol 15 (3) ◽  
Author(s):  
Rossana Psaila ◽  
Donatella Ponti ◽  
Marta Ponzi ◽  
Franca Gigliani ◽  
Piero Battaglia
Keyword(s):  
Ribosomal Rna ◽  
Luciferase Activity ◽  
Protein Biosynthesis ◽  
Base Sequence ◽  
Cell Determination ◽  
Pole Cell ◽  
Higher Eukaryotes ◽  
Domain V ◽  
Drosophila Embryos

AbstractThe mechanism by which the mitochondrial large rRNA is involved in the restoration of the pole cell-forming ability in Drosophila embryos is still unknown. We identified a 15-ribonucleotide sequence which is conserved from the protobacterium Wolbachia to the higher eukaryotes in domain V of the mitochondrial large rRNA. This short sequence is sufficient to restore pole cell determination in UV-irradiated Drosophila embryos. Here, we provide evidence that the conserved 15-base sequence is sufficient to restore luciferase activity in vitro. Moreover, we show that the internal GAGA sequence is involved in protein binding and that mutations in this tetranucleotide affect the sequence’s ability to restore luciferase activity. The obtained results lead us to propose that mtlrRNA may be involved either in damaged protein reactivation or in protein biosynthesis during pole cell determination.

scholarly journals Influence of a 23S ribosomal RNA mutation in Helicobacter pylori strains on the in vitro synergistic effect of clarithromycin and amoxicillin

2012 ◽  
Vol 5 (1) ◽  
Author(s):  
Türkan Sakinc ◽  
Barbara Baars ◽  
Nicole Wüppenhorst ◽  
Manfred Kist ◽  
Johannes Huebner ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Synergistic Effect ◽  
Ribosomal Rna ◽  
23S Ribosomal Rna

Protein folding in Escherichia coli: role of 23S ribosomal RNA

1999 ◽  
Vol 1429 (2) ◽  
pp. 293-298 ◽  
Author(s):  
Subrata Chattopadhyay ◽  
Saumen Pal ◽  
Debashis Pal ◽  
Dibyendu Sarkar ◽  
Suparna Chandra ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein Folding ◽  
Ribosomal Rna ◽  
23S Ribosomal Rna

scholarly journals A new approach to the analysis of hybridization of bacterial nucleic acids. Analysis of ribosomal ribonucleic acids of Bacillus subtilis

1969 ◽  
Vol 115 (3) ◽  
pp. 383-394 ◽  
Author(s):  
R. J. Avery ◽  
J. E. M. Midgley
Keyword(s):  
Bacillus Subtilis ◽  
Ribosomal Rna ◽  
Weight Ratio ◽  
Single Species ◽  
Mass Action ◽  
Analytical Technique ◽  
Dna And Rna ◽  
Bacterial Rna ◽  
Hybridization Efficiency ◽  
23S Ribosomal Rna

A new graphical analytical technique is described for the hybridization of bacterial RNA with denatured homologous DNA immobilized on cellulose nitrate membrane filters. To a constant amount of DNA, various amounts of bacterial RNA were added and the percentage of input RNA bound was plotted against the DNA/RNA weight ratio in a given experiment. When RNA samples were used that hybridize to denatured DNA as a single species, the resulting curves (RNA-hybridization-efficiency curves) could be analysed to show the percentage of the DNA capable of specifically binding the RNA and could also be used to detect the presence of minor RNA contaminants in a purified specimen. The method could also estimate the relative amounts of two species of RNA in a mixture when these were hybridized independently to different DNA cistrons or cistron groups. As an example of RNA that can be studied in this way, the 16s and 23s ribosomal RNA species of Bacillus subtilis were chosen. These each behave in DNA–RNA hybridization as a single species and bind independently to different groups of DNA cistrons. The results obtained from hybridization-efficiency curves were compared with those obtained by the more usual method of saturating the specific DNA regions with excess of ribosomal RNA (hybridization-saturation curves). It was confirmed by both approaches that 0·15 (±0·02)% of B. subtilis DNA would hybridize with 16s ribosomal RNA, 0·30 (±0·02)% would hybridize with 23s ribosomal RNA, and 0·46 (±0·02)% would hybridize with (16s+23s) ribosomal RNA. This agreement suggested that mass-action equilibria between hybridized and free RNA had a negligible effect on the hybridization curves over the range of DNA and RNA concentrations employed.

scholarly journals Mutations in domain V of Mycoplasma pneumoniae 23S rRNA and clinical characteristics of pediatric M. pneumoniae pneumonia in Nanjing, China

2021 ◽  
Vol 49 (6) ◽  
pp. 030006052110163
Author(s):  
Changdi Xu ◽  
Huan Deng ◽  
Jiamin Zhang ◽  
Yifan Zhu ◽  
Qiangquan Rong ◽  
...  
Keyword(s):  
Hospital Stay ◽  
Mycoplasma Pneumoniae ◽  
Ribosomal Rna ◽  
Clinical Characteristics ◽  
Laboratory Data ◽  
Pulmonary Complications ◽  
23S Rrna ◽  
High Prevalence ◽  
Domain V ◽  
23S Ribosomal Rna

Objective To investigate the prevalence of mutations in domain V of Mycoplasma pneumoniae (MP) 23S ribosomal RNA (rRNA) and the clinical characteristics of pediatric MP pneumonia (MPP) in Nanjing, China. Methods Domain V of 23S rRNA was sequenced in MP strains collected from children diagnosed with MPP in Nanjing. Clinical and laboratory data were obtained. Results Among the 276 MP strains, 255 (92.39%) harbored mutations, primarily A2063G in domain V of MP 23S rRNA. When children were stratified according to the presence or absence of mutations, no significant differences were found in sex, age, the MP DNA load at enrollment, lymphocyte counts, pulmonary complications, immunomodulator levels, fever duration, the duration of fever after macrolide therapy, and hospital stay. The prevalence of refractory MPP in the two groups was similar. Children with refractory MPP exhibited higher MP DNA loads than those with non-refractory MPP. Conclusions Despite the high prevalence of the A2063G mutation in domain V of MP 23S rRNA, mutations were not associated with the clinical characteristics of MPP. The MP DNA load significantly differed between refractory and non-refractory MPP.

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