scholarly journals Site-directed mutagenesis of the χ subunit of DNA polymerase III and single-stranded DNA-binding protein of E. coli reveals key residues for their interaction

2010 ◽  
Vol 39 (4) ◽  
pp. 1398-1407 ◽  
Author(s):  
Natalie Naue ◽  
Roman Fedorov ◽  
Andreas Pich ◽  
Dietmar J. Manstein ◽  
Ute Curth
Keyword(s):  
Dna Binding ◽  
Dna Polymerase ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Dna Polymerase Iii ◽  
E Coli ◽  
Polymerase Iii ◽  
Key Residues

The role of gene A protein and E. coli rep protein in the replication of ɸX 174 replicative form DNA

1980 ◽  
Vol 210 (1180) ◽  
pp. 337-349 ◽  
Keyword(s):  
Dna Binding ◽  
Dna Polymerase ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Origin Of Replication ◽  
Electron Microscopic ◽  
Single Stranded Dna ◽  
Rep Protein ◽  
E Coli

The gene A protein of bacteriophage ɸX 174 initiates replication of super-twisted RFI DNA by cleaving the viral (+) strand at the origin of replication and binding to the 5' end. Upon addition of E. coli rep protein (single-stranded DNA dependent ATPase), E. coli single-stranded DNA binding protein and ATP, complete unwinding of the two strands occurs. Electron microscopic analyses of intermediates in the reaction reveal that the unwinding occurs by movement of the 5' end into the duplex, displacing the viral strand in the form of a single-stranded loop. Since unwinding will not occur in the absence of either gene A protein or rep protein, it is presumed that the rep protein interacts to form a complex with the bound gene A protein. Single-stranded DNA binding protein facilitates the unwinding by binding to the exposed single-stranded DNA. Further addition of the four deoxyribotriphosphates and DNA polymerase III holoenzyme to the reaction results in synthesis of viral (+) single-stranded circles in amounts exceeding that of the input template. A model describing the role of gene A protein and rep protein in duplex DNA replication is presented and other properties of gene A protein discussed.

scholarly journals Distinct Double- and Single-Stranded DNA Binding of E. coli Replicative DNA Polymerase III α Subunit

2008 ◽  
Vol 3 (9) ◽  
pp. 577-587 ◽  
Author(s):  
Micah J. McCauley ◽  
Leila Shokri ◽  
Jana Sefcikova ◽  
Česlovas Venclovas ◽  
Penny J. Beuning ◽  
...  
Keyword(s):  
Dna Binding ◽  
Dna Polymerase ◽  
Dna Polymerase Iii ◽  
Single Stranded Dna ◽  
Α Subunit ◽  
E Coli ◽  
Polymerase Iii

Site-directed mutagenesis with Escherichia coli DNA polymerase III holoenzyme

Gene ◽  
1988 ◽  
Vol 62 (1) ◽  
pp. 135-139 ◽  
Author(s):  
Naoya Tsurusluta ◽  
Hisaji Maki ◽  
Laurence Jay Korn
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Dna Polymerase Iii ◽  
Polymerase Iii

scholarly journals Simultaneous Interaction of E. Coli Single Stranded DNA Binding Protein and Replicativedna Polymerase III Alpha Subunit with Single-Stranded DNA Molecules

2013 ◽  
Vol 104 (2) ◽  
pp. 74a
Author(s):  
Kiran Pant ◽  
Susie Nimitpattana ◽  
Michelle C. Silva ◽  
Jana Sefcikova ◽  
Penny J. Beuning ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Alpha Subunit ◽  
Dna Molecules ◽  
Single Stranded Dna ◽  
E Coli ◽  
Polymerase Iii
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