Three-Dimensional Ultrastructure of the Proximal Portion of the Transverse Muscle of the Mouse Tongue: Reconstructed from Transmission Electron Micrographs

Procedures for three-dimensional reconstruction of spherical viruses by Fourier synthesis from electron micrographs

Philosophical Transactions of the Royal Society of London Series B Biological Sciences ◽

10.1098/rstb.1971.0054 ◽

1971 ◽

Vol 261 (837) ◽

pp. 221-230 ◽

Cited By ~ 283

Keyword(s):

Electron Micrographs ◽

Fourier Transforms ◽

Three Dimensional ◽

Transmission Electron Micrographs ◽

Three Dimensional Reconstruction ◽

Fourier Synthesis ◽

Combine Data ◽

Density Maps ◽

Transmission Electron ◽

Dimensional Reconstruction

An account is given of a method which has been developed for computing three-dimensional density maps from transmission electron micrographs using Fourier transforms. The reconstructions objectively combine data from several different views of one or more particles. The application to negatively stained tomato bushy stunt virus is described in detail and the resulting reconstruction presented. Projections of the reconstruction in the appropriate directions agree well with images of the virus taken from micrographs.

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Identification of the three-dimensional gel microstructure from transmission electron micrographs

Journal of Microscopy ◽

10.1111/j.1365-2818.2007.01711.x ◽

2007 ◽

Vol 225 (1) ◽

pp. 10-21 ◽

Cited By ~ 10

Author(s):

RASMUS NISSLERT ◽

MATS KVARNSTRÖM ◽

NIKLAS LORÉN ◽

MAGNUS NYDÉN ◽

MATS RUDEMO

Keyword(s):

Electron Micrographs ◽

Three Dimensional ◽

Transmission Electron Micrographs ◽

Transmission Electron

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A THREE-DIMENSIONAL COMPUTER MODEL OF THE TRACHEID CELL WALL AS A TOOL FOR INTERPRETATION OF WOOD CELL WALL ULTRASTRUCTURE

IAWA Journal ◽

10.1163/22941932-90000280 ◽

2001 ◽

Vol 22 (3) ◽

pp. 213-233 ◽

Cited By ~ 9

Author(s):

L.A. Donaldson

Keyword(s):

Cell Wall ◽

Electron Micrographs ◽

Cell Walls ◽

Three Dimensional ◽

Transmission Electron Micrographs ◽

Direct Result ◽

Thin Sections ◽

Transmission Electron ◽

Wall Ultrastructure ◽

Random Patterns

Three-dimensional computer models were used to simulate transmission electron micrographs in order to determine the effects of changes in microfibril orientation and arrangement on the appearance of ultrastructural images based on thin sections. It is shown that the tangential fibrillar texture commonly associated with wood cell walls results not from individual microfibrils arranged in tangential lamellae but from overlapping of adjacent microfibrils irrespective of their arrangement. The tangential lamellae observed in transmission electron micrographs of wood cell walls do not necessarily reflect the underlying nanostructure of the wall. Tangential textures can occur irrespective of the arrangement of microfibrils in tangential, radial or random patterns as a direct result of the helical organisation of the cell wall. Comparison between model images and high resolution micrographs suggests that microfibrils are arranged randomly in weakly defined clusters, with perhaps varying amounts of tangential or radial organisation.

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