Three-Dimensional Structure of Intracellular Membranous Organelles in Embryonic Chick Skeletal Muscle Cells in vitro Revealed with Scanning Electron Microscope

1990 ◽  
Keyword(s):  
Skeletal Muscle ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Dimensional Structure ◽  
Embryonic Chick ◽  
Scanning Electron

scholarly journals THE FINE STRUCTURE OF EMBRYONIC CHICK SKELETAL MUSCLE CELLS DIFFERENTIATED IN VITRO

1967 ◽  
Vol 35 (2) ◽  
pp. 445-453 ◽  
Author(s):  
Y. Shimada ◽  
D. A. Fischman ◽  
A. A. Moscona
Keyword(s):  
Electron Microscopy ◽  
Skeletal Muscle ◽  
Fine Structure ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Chick Embryos ◽  
Embryonic Chick ◽  
Developing Muscle

Dissociated myoblasts from 12-day chick embryos were cultured in monolayer, and the differentiation of skeletal muscle cells was studied by electron microscopy. The results have revealed a striking ultrastructural similarity between the in vivo and the in vitro developing muscle, particularly with respect to the myofibrils and sarcoplasmic reticulum. This study demonstrates that all the characteristic organelles of mature skeletal muscle can develop in vitro in the absence of nerves.

scholarly journals Troponin in embryonic chick skeletal muscle cells in vitro. An immunoelectron microscope study.

1979 ◽  
Vol 81 (1) ◽  
pp. 59-66 ◽  
Author(s):  
T Obinata ◽  
Y Shimada ◽  
R Matsuda
Keyword(s):  
Skeletal Muscle ◽  
Troponin T ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Regulatory Proteins ◽  
Embryonic Chick ◽  
Thin Filaments ◽  
Adult Chicken ◽  
Chicken Muscles

The fine structurel distribution of troponin on thin filaments in developing myofibrils was investigated by the use of immunoelectron microscopy. Embryonic chick skeletal muscle cells grown in vitro were treated with antibodies against each of the troponin components (troponin T, I, and C) from adult chicken muscles. Each antibody was distributed along the thin filaments with a period of 38 nm. It is concluded that these newly synthesized regulatory proteins are assembled at their characteristic position from the initial phases of myofibrillogenesis.

A scanning electron microscope study of Cucurbita maxima seed coat structure

1973 ◽  
Vol 51 (10) ◽  
pp. 1711-1714 ◽  
Author(s):  
John N. A. Lott
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Seed Coat ◽  
Three Dimensional ◽  
Deposition Process ◽  
Dimensional Structure ◽  
Cucurbita Maxima ◽  
Spongy Parenchyma ◽  
Scanning Electron Microscope Study ◽  
Scanning Electron

The examination of the seed coat of Cucurbita maxima with the scanning electron microscope provided information about the three-dimensional structure of the seed coat cells. The lumpy appearance of the spongy parenchyma cells indicated that localized wall growth must have occurred. Also of particular interest were the reticulate secondary wall thickenings in the hypodermal and spongy parenchyma regions of the seed coat. The developing squash seed coat may prove to be a good model system in which to study the cell wall deposition process.

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