scholarly journals A new strategy for species identification of planktonic larvae: PCR–RFLP analysis of the internal transcribed spacer region of ribosomal DNA detected by agarose gel electrophoresis or DHPLC

2006 ◽  
Vol 28 (4) ◽  
pp. 375-384 ◽  
Author(s):  
Shi Wang ◽  
Zhenmin Bao ◽  
Lingling Zhang ◽  
Ning Li ◽  
Aibin Zhan ◽  
...  
Keyword(s):  
Species Identification ◽  
Ribosomal Dna ◽  
Gel Electrophoresis ◽  
Internal Transcribed Spacer ◽  
Rflp Analysis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Internal Transcribed Spacer Region ◽  
Pcr Rflp ◽  
New Strategy

scholarly journals Molecular and Biochemical Methods Used for the Identification of Globodera Species in Slovenia (from 6th Conference of European Foundation for Plant Pathology, Prague, Czech Republic, 8–14 September 2002)

2011 ◽  
Vol 39 (No. 4) ◽  
pp. 151-153
Author(s):  
S. Širca ◽  
G. Urek ◽  
V. Meglič
Keyword(s):  
Plant Pathology ◽  
Czech Republic ◽  
Gel Electrophoresis ◽  
Morphological Characteristics ◽  
Protein Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Biochemical Methods ◽  
Pcr Rflp ◽  
European Foundation

Globodera species identification is based on specific morphological characteristics which are included in the majority of keys used for Globodera identification. To confirm and make more precise the morphometrical method, other methods have been developed. At Agricultural Institute of Slovenia, molecular and biochemical methods are applied. With the use of PCR-RFLP method we try to distinguish between G. rostochiensis, G. pallida and G. tabacum. Patterns of nematode DNA digested with restriction endonucleases and subjected to agarose gel electrophoresis are analysed. Differences in DNA sequence result in the number and size of fragments produced (RFLPs). For the differentiation of all three nematodes we have introduced a method for protein electrophoresis. Samples are compared after being separated by polyacrilamide slab gel electrophoresis. Proteins are visualised after silver staining.        

Genetic diversity among a complex of cereal cyst nematodes inferred from RFLP analysis of the ribosomal internal transcribed spacer region

Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 479-486 ◽  
Author(s):  
S. Bekal ◽  
J. P. Gauthier ◽  
R. Rivoal
Keyword(s):  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Rflp Analysis ◽  
Restriction Pattern ◽  
Nuclear Ribosomal Dna ◽  
Heterodera Avenae ◽  
Cyst Nematodes ◽  
Internal Transcribed Spacer Region ◽  
5.8S Rdna ◽  
Pcr Rflp

This study examined the restriction polymorphism (RFLP) of the nuclear ribosomal DNA in Heterodera avenae, H. filipjevi, H. mani, H. latipons, and the taxonomically unclear Gotland strain in order to establish a molecular characterization and phylogenetic relationships in the complex of cereal cyst nematodes (CCN). The internal transcribed spacer (ITS) and 5.8S rDNA were amplified by PCR from a single female or a cyst of 27 different geographic isolates of the CCN complex and one population of H. schachtii, used as outgroup. The amplified product was 1.2 kb long and 14 of 15 enzymes produced restriction fragments for each isolate. Relationships between populations were determined from UPGMA analysis based on distance values calculated from RFLP data. Digestions with TaqI clearly differentiated H. avenae, H. latipons, and a group composed of H. filipjevi and the Gotland strain. Six endonucleases (HaeIII, HinfI, ItaI, PstI, TaqI, and Tru9I) produced the same restriction pattern with H. filipjevi and the Gotland strain, and both were clearly separated from H. avenae with PstI. Restriction sites have revealed a mixture of the species H. latipons and H. avenae, and possible infraspecific variation in H. avenae. The inferred phylogenetic relationships of species in the CCN complex are in agreement with their morphological characterization.Key words: cereal cyst nematodes, Heterodera avenae, PCR, RFLP, ribosomal diversity.

scholarly journals Frequencies of CYP2B6∗4,∗5, and ∗6 Alleles within an Iranian Population (Mazandaran)

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Mohammad Bagher Hashemi-Soteh ◽  
Elaheh Hosseini ◽  
Shokoufeh Fazelnia ◽  
Faramarz Ghasemian-Sorbeni ◽  
Sara Madahian ◽  
...  
Keyword(s):  
Ethnic Group ◽  
Ethnic Diversity ◽  
Gel Electrophoresis ◽  
Restriction Enzymes ◽  
Iranian Population ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Cytochrome P450 2B6 ◽  
Pcr Product ◽  
Pcr Rflp

Background. The human CYP2B subfamily consists of one functional gene (CYP2B6) and one pseudogene (CYP2B7P). Cytochrome P450 2B6 (CYP2B6) is a highly polymorphic enzyme that shows marked interindividual and interethnic variations. Currently, 38 alleles have been described, and some of the allelic variants have been associated with low enzyme activity. The aim of this study was to investigate the frequencies of CYP2B6∗4, CYP2B6∗5, and CYP2B6∗6 alleles in the Mazani ethnic group among Iranian Population. Methods. The study was conducted in 289 unrelated healthy volunteers. DNA was extracted from peripheral blood and analyzed by the PCR-RFLP protocol. The PCR product was digested with restriction enzymes and then separated using agarose gel electrophoresis. Results. The frequency of CYP2B6∗4, CYP2B6∗5, and CYP2B6∗6 in this study was 34.60%, 7.26%, and 34.54%, respectively. Conclusion. The frequency of the CYP2B6∗4 allele in the Mazani ethnic group was much higher (34.60%) than other population. The frequency of CYP2B6∗6 (34.54%) also was higher than its frequency in other previously reported population. But the frequency of CYP2B6∗5 in this study was lower than expected. These results will be useful in understanding the ethnic diversity in Iranian population and offer a preliminary basis for more rational use of drugs that are substrates for CYP2B6 in this population.

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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