scholarly journals Fusion of a recombinant antibody fragment with a homo-amino-acid polymer: effects on biophysical properties and prolonged plasma half-life

2007 ◽  
Vol 20 (6) ◽  
pp. 273-284 ◽  
Author(s):  
M. Schlapschy ◽  
I. Theobald ◽  
H. Mack ◽  
M. Schottelius ◽  
H.-J. Wester ◽  
...  
Keyword(s):  
Amino Acid ◽  
Half Life ◽  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Biophysical Properties ◽  
Recombinant Antibody Fragment ◽  
Plasma Half Life

High-level accumulation of a recombinant antibody fragment in the periplasm ofEscherichia coli requires a triple-mutant (degP prc spr) host strain

2004 ◽  
Vol 85 (5) ◽  
pp. 463-474 ◽  
Author(s):  
Christina Chen ◽  
Brad Snedecor ◽  
Julie C. Nishihara ◽  
John C. Joly ◽  
Nancy McFarland ◽  
...  
Keyword(s):  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Ofescherichia Coli ◽  
Host Strain ◽  
Triple Mutant ◽  
Recombinant Antibody Fragment ◽  
High Level

Generation and characterization of a recombinant antibody fragment that binds to the coat protein of grapevine leafroll-associated virus 3

2008 ◽  
Vol 153 (6) ◽  
pp. 1075-1084 ◽  
Author(s):  
Martin Orecchia ◽  
Greta Nölke ◽  
Pasquale Saldarelli ◽  
Mariangela Dell’Orco ◽  
Kerstin Uhde-Holzem ◽  
...  
Keyword(s):  
Coat Protein ◽  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Recombinant Antibody Fragment

scholarly journals Isolation and Characterisation of a Recombinant Antibody Fragment That Binds NCAM1-Expressing Intervertebral Disc Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e83678 ◽  
Author(s):  
Claire Cunningham ◽  
Akshay Srivastava ◽  
Estelle Collin ◽  
Sibylle Grad ◽  
Mauro Alini ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Recombinant Antibody Fragment ◽  
Disc Cells

scholarly journals Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli

Medicina ◽  
2021 ◽  
Vol 57 (9) ◽  
pp. 981
Author(s):  
Chang-Hun Yeom ◽  
Hee-Jin Jeong
Keyword(s):  
Escherichia Coli ◽  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Soluble Form ◽  
Efficient Production ◽  
Binding Ability ◽  
Recombinant Scfv ◽  
Recombinant Antibody Fragment ◽  
Recombinant Antibody Fragments ◽  
Metalloproteinase 9

Matrix metalloproteinase 9 (MMP9) is involved in several aspects of the pathology of cancer, including invasion, metastasis, and angiogenesis. In this study, we expressed a recombinant scFv-type anti-MMP9 antibody in soluble form using Escherichia coli, purified it, and confirmed its antigen-binding ability. The convenient, rapid, inexpressive system used in this study for producing recombinant antibody fragments needs only five days, and thus can be used for the efficient production of scFv against MMP9, which can be used in a range of applications and industrial fields, including diagnosis and treatment of inflammatory and cancer-related diseases.

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