scholarly journals Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ikhlaas M. Kasli ◽  
Owen R. T. Thomas ◽  
Tim W. Overton
Keyword(s):  
Escherichia Coli ◽  
Design Of Experiments ◽  
Signal Peptide ◽  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Recombinant Antibody Fragment ◽  
Selection Of

scholarly journals Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli

Medicina ◽  
2021 ◽  
Vol 57 (9) ◽  
pp. 981
Author(s):  
Chang-Hun Yeom ◽  
Hee-Jin Jeong
Keyword(s):  
Escherichia Coli ◽  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Soluble Form ◽  
Efficient Production ◽  
Binding Ability ◽  
Recombinant Scfv ◽  
Recombinant Antibody Fragment ◽  
Recombinant Antibody Fragments ◽  
Metalloproteinase 9

Matrix metalloproteinase 9 (MMP9) is involved in several aspects of the pathology of cancer, including invasion, metastasis, and angiogenesis. In this study, we expressed a recombinant scFv-type anti-MMP9 antibody in soluble form using Escherichia coli, purified it, and confirmed its antigen-binding ability. The convenient, rapid, inexpressive system used in this study for producing recombinant antibody fragments needs only five days, and thus can be used for the efficient production of scFv against MMP9, which can be used in a range of applications and industrial fields, including diagnosis and treatment of inflammatory and cancer-related diseases.

scholarly journals Expression of Recombinant Antibody Fragment, Anti BNP-SCFV on the Periplasm of Escherichia Coli for the Detection of Heart Failure

Molekul ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 30
Author(s):  
Shabarni Gaffar ◽  
Sofyan Multazam N Aji ◽  
Yeni W Hartati ◽  
Safri Ishmayana ◽  
Toto Subroto
Keyword(s):  
Heart Failure ◽  
Escherichia Coli ◽  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Single Chain ◽  
Recombinant Antibody Fragment ◽  
Sds Page ◽  
Patients With Heart Failure ◽  
Heart Muscle Cells ◽  
Antigen Antibody

Basic natriuretic peptide (BNP) is a polypeptide hormone consist of 32 amino acids that secreted by the heart ventricle to respond the excessive stretching of heart muscle cells. BNP can be used as prognostic marker for patients with heart failure. The presence of BNP in blood can be detected by BNP antibody, which is anti BNP-single chain variable fragment (Anti BNP-SCFV). The antibody is a combination of polypeptides between varying region on the heavy chain (VH) and the light chain (VL) of immunoglobulin. Anti BNP-SCFV will bind to BNP through the antigen-antibody interaction. Concentration of BNP in a patient’s blood can be detected through the interaction of BNP with Anti BNP-SCFV using immunosensor method. Production of recombinant Anti BNP-SCFV in Escherichia coli as host is reported in the present study. Anti BNP-SCFV was expressed in fusion form with OmpC signal peptide that direct the protein to a periplasmic space. Expression was performed under RhaBad promoter as control using L-rhamnose as inducer. SDS-PAGE characterization showed consistent band at 28 kDa, which was assumed as Anti BNP-SCFV. The optimum expression was found at four hours after induction with 4 mM inducer. Anti BNP-SCFV was secreted from the cell as characterized by the presence of the protein on periplasmic membrane and extracellular fraction.

High-level accumulation of a recombinant antibody fragment in the periplasm ofEscherichia coli requires a triple-mutant (degP prc spr) host strain

2004 ◽  
Vol 85 (5) ◽  
pp. 463-474 ◽  
Author(s):  
Christina Chen ◽  
Brad Snedecor ◽  
Julie C. Nishihara ◽  
John C. Joly ◽  
Nancy McFarland ◽  
...  
Keyword(s):  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Ofescherichia Coli ◽  
Host Strain ◽  
Triple Mutant ◽  
Recombinant Antibody Fragment ◽  
High Level

Evaluation of biofilm performance as a protective barrier against biocorrosion using an enzyme electrode

2011 ◽  
Vol 64 (8) ◽  
pp. 1736-1742 ◽  
Author(s):  
S. Soleimani ◽  
B. Ormeci ◽  
O. B. Isgor ◽  
S. Papavinasam
Keyword(s):  
Escherichia Coli ◽  
Growth Conditions ◽  
Microbiologically Influenced Corrosion ◽  
Simple Method ◽  
E Coli ◽  
Concrete Deterioration ◽  
Protective Barrier ◽  
Microbial Biofilms ◽  
Short Time ◽  
Selection Of

Sulfide is known to be an important factor in microbiologically influenced corrosion (MIC) of metals and concrete deterioration in wastewater treatment structures and sewer pipelines. A sulfide biosensor was used to determine the effectiveness of Escherichia coli DH5α biofilm as a protective barrier against MIC. The biofilm was shown to be effective in protecting surfaces from sulfide and helping to reduce MIC using amperometric measurements. The results also indicated that the growth conditions of E. coli DH5α may have an impact on the performance of the biofilm as a sulfide barrier. The simple method provided in this work enables the comparison of several microbial biofilms and selection of the ones with potential to prevent MIC in a relatively short time.

Generation and characterization of a recombinant antibody fragment that binds to the coat protein of grapevine leafroll-associated virus 3

2008 ◽  
Vol 153 (6) ◽  
pp. 1075-1084 ◽  
Author(s):  
Martin Orecchia ◽  
Greta Nölke ◽  
Pasquale Saldarelli ◽  
Mariangela Dell’Orco ◽  
Kerstin Uhde-Holzem ◽  
...  
Keyword(s):  
Coat Protein ◽  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Recombinant Antibody Fragment
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