We studied the distribution of laminin (Ln) α1–α3, β1–β3, and γ1 chains, and of the extradomain-A (EDA) and EDB and the oncofetal epitope of fibronectin (Onc-Fn) in extravillous trophoblastic cells and decidua in the human placenta by immunohistochemistry. We found that the transition from villous to extravillous trophoblast was accompanied by emergence of immunoreactivity for EDA-, EDB-, and Onc-Fn among the cells. Furthermore, whereas the villous trophoblastic basement membrane (BM) contains Ln α1, α2, β1, β2, and γ1 chains, immunoreactivity for Ln α1, β1, and γ1, but not for Ln α2 and β2 chains, was detected in association with extravillous trophoblastic cells. Interestingly, although immunoreactivity for the Ln α1, α2, β1, β2, and γ1 chains was detected in all decidual cell BMs, EDB-Fn and Onc-Fn were detected only in decidua that had been invaded by the trophoblast. In summary, our results describe distinct changes in the distribution of Ln and Fn isoforms during the differentiation of villous trophoblast into extravillous trophoblastic cells. Furthermore, EDB- and Onc-Fn are preferentially found in decidua that has been invaded by the trophoblast, indicating that the deposition of these Fn isoforms reflects a decidual cell response to invasion.
Ketoconazole Impairs Early Pregnancy and the Decidual Cell Response via Alterations in Ovarian Function