scholarly journals Nitric Oxide Synthase: “Enzyme Zero” in Air Pollution–Induced Vascular Toxicity

2009 ◽  
Vol 110 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Matthew J. Campen
Keyword(s):  
Nitric Oxide ◽  
Air Pollution ◽  
Nitric Oxide Synthase ◽  
Vascular Toxicity ◽  
Oxide Synthase

Air Pollution Is Associated With DNA Methylation In Nitric Oxide Synthase

2011 ◽  
Author(s):  
Carrie Breton ◽  
Hyang-Min Byun ◽  
Xinhui Wang ◽  
Muhammad T. Salam ◽  
Kimberly D. Siegmund ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Dna Methylation ◽  
Air Pollution ◽  
Nitric Oxide Synthase ◽  
Oxide Synthase

Air pollution alters brain and pituitary endothelin-1 and inducible nitric oxide synthase gene expression

2007 ◽  
Vol 105 (2) ◽  
pp. 224-233 ◽  
Author(s):  
Errol M. Thomson ◽  
Prem Kumarathasan ◽  
Lilian Calderón-Garcidueñas ◽  
Renaud Vincent
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Air Pollution ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Endothelin 1 ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Exposure to traffic-related air pollution and changes in exhaled nitric oxide and DNA methylation in arginase and nitric oxide synthase in children with asthma

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
N. Ji ◽  
M. Fang ◽  
A. Baptista ◽  
C. Cepeda ◽  
M. Greenberg ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Dna Methylation ◽  
Air Pollution ◽  
Nitric Oxide Synthase ◽  
Airway Inflammation ◽  
Personal Exposure ◽  
Epigenetic Changes ◽  
Promoter Regions ◽  
Children With Asthma ◽  
Oxide Synthase

Abstract Background Traffic-related air pollution (TRAP) has been associated with increased risk of airway inflammation in children with asthma. While epigenetic changes could potentially modulate TRAP-induced inflammatory responses, few studies have assessed the temporal pattern of exposure to TRAP, epigenetic changes and inflammation in children with asthma. Our goal was to test the time-lag patterns of personal exposure to TRAP, airway inflammation (measured as fractional exhaled nitric oxide, FeNO), and DNA methylation in the promoter regions of genes involved in nitric oxide synthesis among children with asthma. Methods We measured personal exposure to black carbon (BC) and FeNO for up to 30 days in a panel of children with asthma. We collected 90 buccal cell samples for DNA methylation analysis from 18 children (5 per child). Methylation in promoter regions of nitric oxide synthase (NOS1, NOS2A, NOS3) and arginase (ARG1, ARG2) was assessed by bisulfite pyrosequencing. Linear-mixed effect models were used to test the associations of BC at different lag periods, percent DNA methylation at each site and FeNO level. Results Exposure to BC was positively associated with FeNO, and negatively associated with DNA methylation in NOS3. We found strongest association between FeNO and BC at lag 0–6 h while strongest associations between methylation at positions 1 and 2 in NOS3 and BC were at lag 13–24 h and lag 0–24 h, respectively. The strengths of associations were attenuated at longer lag periods. No significant associations between exposure to TRAP and methylation levels in other NOS and ARG isoforms were observed. Conclusions Exposure to TRAP was associated with higher levels of FeNO and lower levels of DNA methylation in the promoter regions of the NOS3 gene, indicating that DNA methylation of the NOS3 gene could be an important epigenetic mechanism in physiological responses to TRAP in children with asthma.

scholarly journals Exposure to Traffic-Related Air Pollution and Changes in Exhaled Nitric Oxide and DNA Methylation in Arginase and Nitric Oxide Synthase in Children with Asthma

2020 ◽  
Author(s):  
Nan Ji ◽  
Mingzhu Fang ◽  
Ana Baptista ◽  
Clarimel Cepeda ◽  
Molly Greenberg ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Dna Methylation ◽  
Air Pollution ◽  
Nitric Oxide Synthase ◽  
Personal Exposure ◽  
Epigenetic Changes ◽  
Promoter Regions ◽  
Nos3 Gene ◽  
Children With Asthma ◽  
Oxide Synthase

Abstract Background Traffic-related air pollution (TRAP) has been associated with increased risk of airway inflammation in children with asthma. While epigenetic changes could potentially modulate TRAP-induced inflammatory responses, few studies have assessed the temporal pattern of exposure to TRAP, epigenetic changes and inflammation in children with asthma. Our goal was to test the time-lag patterns of personal exposure to TRAP, airway inflammation (measured as fractional exhaled nitric oxide, FeNO), and DNA methylation in the promoter regions of genes involved in nitric oxide synthesis among children with asthma. Methods We measured personal exposure to black carbon (BC) and FeNO for up to 30 days in a panel of children with asthma. We collected 90 buccal cell samples for DNA methylation analysis from 18 children (5 per child). Methylation in promoter regions of nitric oxide synthase (NOS1, NOS2A, NOS3) and arginase (ARG1, ARG2) was assessed by bisulfite pyrosequencing. Linear-mixed effect models were used to test the associations of BC at different lag periods, percent DNA methylation at each site and FeNO level. Results Exposure to BC was positively associated with FeNO, and negatively associated with DNA methylation in NOS3. We found strongest association between FeNO and BC at lag 0–6 hours while strongest associations between methylation at positions 1 and 2 in NOS3 and BC were at lag 13–24 hours and lag 0–24 hours, respectively. The strengths of associations were attenuated at longer lag periods. No significant associations between exposure to TRAP and methylation levels in other NOS and ARG isoforms were observed. Conclusions Exposure to TRAP was associated with higher levels of FeNO and lower levels of DNA methylation in the promoter regions of the NOS3 gene, indicating that DNA methylation of the NOS3 gene could be an important epigenetic mechanism in physiological responses to TRAP in children with asthma.

scholarly journals Exposure to Traffic-Related Air Pollution and Changes in Exhaled Nitric Oxide and DNA Methylation in Arginase and Nitric Oxide Synthase in Children with Asthma

2020 ◽  
Author(s):  
Nan Ji ◽  
Mingzhu Fang ◽  
Ana Baptista ◽  
Clarimel Cepeda ◽  
Molly Greenberg ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Dna Methylation ◽  
Air Pollution ◽  
Nitric Oxide Synthase ◽  
Airway Inflammation ◽  
Personal Exposure ◽  
Epigenetic Changes ◽  
Promoter Regions ◽  
Children With Asthma ◽  
Oxide Synthase

Abstract Background: Traffic-related air pollution (TRAP) has been associated with increased risk of airway inflammation in children with asthma. While epigenetic changes could potentially modulate TRAP-induced inflammatory responses, few studies have assessed the temporal pattern of exposure to TRAP, epigenetic changes and inflammation in children with asthma. Our goal was to test the time-lag patterns of personal exposure to TRAP, airway inflammation (measured as fractional exhaled nitric oxide, FeNO), and DNA methylation in the promoter regions of genes involved in nitric oxide synthesis among children with asthma.Methods: We measured personal exposure to black carbon (BC) and FeNO for up to 30 days in a panel of children with asthma. We collected 90 buccal cell samples for DNA methylation analysis from 18 children (5 per child). Methylation in promoter regions of nitric oxide synthase (NOS1, NOS2A, NOS3) and arginase (ARG1, ARG2) was assessed by bisulfite pyrosequencing. Linear-mixed effect models were used to test the associations of BC at different lag periods, percent DNA methylation at each site and FeNO level.Results: Exposure to BC was positively associated with FeNO, and negatively associated with DNA methylation in NOS3. We found strongest association between FeNO and BC at lag 0–6 hours while strongest associations between methylation at positions 1 and 2 in NOS3 and BC were at lag 13-24 hours and lag 0-24 hours, respectively. The strengths of associations were attenuated at longer lag periods. No significant associations between exposure to TRAP and methylation levels in other NOS and ARG isoforms were observed.Conclusions: Exposure to TRAP was associated with higher levels of FeNO and lower levels of DNA methylation in the promoter regions of the NOS3 gene, indicating that DNA methylation of the NOS3 gene could be an important epigenetic mechanism in physiological responses to TRAP in children with asthma.

Localization of endothelial nitric oxide synthase in the normal and failing human atrial myocardium

1996 ◽  
Vol 54 ◽  
pp. 786-787
Author(s):  
Chi-Ming Wei ◽  
Margarita Bracamonte ◽  
Shi-Wen Jiang ◽  
Richard C. Daly ◽  
Christopher G.A. McGregor ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Heart Failure ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Cardiac Tissues ◽  
Mammalian Tissues ◽  
End Stage Heart Failure ◽  
End Stage ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Nitric oxide (NO) is a potent endothelium-derived relaxing factor which also may modulate cardiomyocyte inotropism and growth via increasing cGMP. While endothelial nitric oxide synthase (eNOS) isoforms have been detected in non-human mammalian tissues, expression and localization of eNOS in the normal and failing human myocardium are poorly defined. Therefore, the present study was designed to investigate eNOS in human cardiac tissues in the presence and absence of congestive heart failure (CHF).Normal and failing atrial tissue were obtained from six cardiac donors and six end-stage heart failure patients undergoing primary cardiac transplantation. ENOS protein expression and localization was investigated utilizing Western blot analysis and immunohistochemical staining with the polyclonal rabbit antibody to eNOS (Transduction Laboratories, Lexington, Kentucky).

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