A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence and Structural Similarities Across Host-Specific Lineages

The blast fungus Magnaporthe oryzae (syn. Pyricularia oryzae) is a destructive plant pathogen that can infect about 50 species of both wild and cultivated grasses, including important crops such as rice and wheat. M. oryzae is composed of genetically differentiated lineages that tend to infect specific host genera. To date, most studies of M. oryzae effectors have focused on the rice-infecting lineage. We describe a clone resource of 195 effectors of Magnaporthe species predicted from all the major host-specific lineages. These clones are freely available as Golden Gate–compatible entry plasmids. Our aim is to provide the community with an open source effector clone library to be used in a variety of functional studies. We hope that this resource will encourage studies of M. oryzae effectors on diverse host species.

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The Potential of Streptomyces as Biocontrol Agents against the Rice Blast Fungus, Magnaporthe oryzae (Pyricularia oryzae)

Frontiers in Microbiology ◽

10.3389/fmicb.2017.00003 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 56

Author(s):

Jodi Woan-Fei Law ◽

Hooi-Leng Ser ◽

Tahir M. Khan ◽

Lay-Hong Chuah ◽

Priyia Pusparajah ◽

...

Keyword(s):

Magnaporthe Oryzae ◽

Rice Blast ◽

Rice Blast Fungus ◽

Pyricularia Oryzae ◽

Biocontrol Agents ◽

Blast Fungus

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Evolution of an Avirulence Gene, AVR1-CO39, Concomitant with the Evolution and Differentiation of Magnaporthe oryzae

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-18-1148 ◽

2005 ◽

Vol 18 (11) ◽

pp. 1148-1160 ◽

Cited By ~ 38

Author(s):

Yukio Tosa ◽

Jun Osue ◽

Yukiko Eto ◽

Hong-Sik Oh ◽

Hitoshi Nakayashiki ◽

...

Keyword(s):

Resistance Gene ◽

Ribosomal Dna ◽

Magnaporthe Oryzae ◽

Dna Sequences ◽

Early Stage ◽

Pyricularia Oryzae ◽

Avirulence Gene ◽

Rice Cultivars ◽

Blast Fungus ◽

Specific Subgroup

The significance of AVR1-CO39, an avirulence gene of the blast fungus corresponding to Pi-CO39(t) in rice cultivars, during the evolution and differentiation of the blast fungus was evaluated by studying its function and distribution in Pyricularia spp. When the presence or absence of AVR1-CO39 was plotted on a dendrogram constructed from ribosomal DNA sequences, a perfect parallelism was observed between its distribution and the phylogeny of Pyricularia isolates. AVR1-CO39 homologs were exclusively present in one species, Pyricularia oryzae, suggesting that AVR1-CO39 appeared during the early stage of evolution of P. oryzae. Transformation assays showed that all the cloned homologs tested are functional as an avirulence gene, indicating that selection has maintained their function. Nevertheless, Oryza isolates (isolates virulent on Oryza spp.) in P. oryzae exceptionally noncarriers of AVR1-CO39. All Oryza isolates suffered from one of the two types of known rearrangements at the Avr1-CO39 locus (i.e., G type and J type). These types were congruous to the two major lineages of Oryza isolates from Japan determined by MGR586 and MAGGY. These results indicate that AVR1-CO39 was lost during the early stage of evolution of the Oryza-specific subgroup of P. oryzae. Interestingly, its corresponding resistance gene, Pi-CO39(t), is not widely distributed in Oryza spp.

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Analyzing Avirulence Genes of Magnaporthe oryzae from Heilongjiang Pro- vince and Screening Rice Germplasm with Resistance to Blast Fungus

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2012.02192 ◽

2013 ◽

Vol 38 (12) ◽

pp. 2192-2197 ◽

Cited By ~ 4

Author(s):

Xiang-Xiao LI ◽

Qian WANG ◽

Sheng-Xiang LUO ◽

Yun-Xia HE ◽

Ling-Hua ZHU ◽

...

Keyword(s):

Magnaporthe Oryzae ◽

Avirulence Genes ◽

Rice Germplasm ◽

Blast Fungus

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Investigating the cell and developmental biology of plant infection by the rice blast fungus Magnaporthe oryzae

Fungal Genetics and Biology ◽

10.1016/j.fgb.2021.103562 ◽

2021 ◽

pp. 103562

Author(s):

Alice Bisola Eseola ◽

Lauren S. Ryder ◽

Míriam Osés-Ruiz ◽

Kim Findlay ◽

Xia Yan ◽

...

Keyword(s):

Developmental Biology ◽

Magnaporthe Oryzae ◽

Rice Blast ◽

Rice Blast Fungus ◽

Plant Infection ◽

Blast Fungus

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Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology

Synthetic Biology ◽

10.1093/synbio/ysab003 ◽

2021 ◽

Vol 6 (1) ◽

Author(s):

Marcos Valenzuela-Ortega ◽

Christopher French

Keyword(s):

Copy Number ◽

Life Science ◽

Insertion Site ◽

Golden Gate ◽

Common Elements ◽

Modern Life ◽

Specific Host ◽

Modular Cloning ◽

Golden Gate Cloning ◽

Selection Of

Abstract Generation of new DNA constructs is an essential process in modern life science and biotechnology. Modular cloning systems based on Golden Gate cloning, using Type IIS restriction endonucleases, allow assembly of complex multipart constructs from reusable basic DNA parts in a rapid, reliable and automation-friendly way. Many such toolkits are available, with varying degrees of compatibility, most of which are aimed at specific host organisms. Here, we present a vector design which allows simple vector modification by using modular cloning to assemble and add new functions in secondary sites flanking the main insertion site (used for conventional modular cloning). Assembly in all sites is compatible with the PhytoBricks standard, and vectors are compatible with the Standard European Vector Architecture (SEVA) as well as BioBricks. We demonstrate that this facilitates the construction of vectors with tailored functions and simplifies the workflow for generating libraries of constructs with common elements. We have made available a collection of vectors with 10 different microbial replication origins, varying in copy number and host range, and allowing chromosomal integration, as well as a selection of commonly used basic parts. This design expands the range of hosts which can be easily modified by modular cloning and acts as a toolkit which can be used to facilitate the generation of new toolkits with specific functions required for targeting further hosts.

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Tanzawaic acids I–L: Four new polyketides from Penicillium sp. IBWF104-06

Beilstein Journal of Organic Chemistry ◽

10.3762/bjoc.10.20 ◽

2014 ◽

Vol 10 ◽

pp. 251-258 ◽

Cited By ~ 8

Author(s):

Louis P Sandjo ◽

Eckhard Thines ◽

Till Opatz ◽

Anja Schüffler

Keyword(s):

Soil Sample ◽

Magnaporthe Oryzae ◽

Rice Blast ◽

Rice Blast Fungus ◽

Fungal Strain ◽

Conidial Germination ◽

Penicillium Sp ◽

Culture Filtrates ◽

Blast Fungus ◽

New Compounds

Four new polyketides have been identified in culture filtrates of the fungal strain Penicillium sp. IBWF104-06 isolated from a soil sample. They are structurally based on the same trans-decalinpentanoic acid skeleton as tanzawaic acids A–H. One of the new compounds was found to inhibit the conidial germination in the rice blast fungus Magnaporthe oryzae at concentrations of 25 μg/mL.

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