scholarly journals The Helper Component Proteinase Cistron of Potato virus Y Induces Hypersensitivity and Resistance in Potato Genotypes Carrying Dominant Resistance Genes on Chromosome IV

2011 ◽  
Vol 24 (7) ◽  
pp. 787-797 ◽  
Author(s):  
Benoît Moury ◽  
Bernard Caromel ◽  
Elisabeth Johansen ◽  
Vincent Simon ◽  
Laura Chauvin ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Dominant Gene ◽  
Recombination Breakpoint ◽  
Nucleotide Substitutions ◽  
Helper Component ◽  
Systemic Movement ◽  
Recent Emergence ◽  
Clade C ◽  
Helper Component Proteinase

The Nctbr and Nytbr genes in Solanum tuberosum determine hypersensitive reactions, characterized by necrotic reactions and restriction of the virus systemic movement, toward isolates belonging to clade C and clade O of Potato virus Y (PVY), respectively. We describe a new resistance from S. sparsipilum which possesses the same phenotype and specificity as Nctbr and is controlled by a dominant gene designated Ncspl. Ncspl maps on potato chromosome IV close or allelic to Nytbr. The helper component proteinase (HC-Pro) cistron of PVY was shown to control necrotic reactions and resistance elicitation in plants carrying Ncspl, Nctbr, and Nytbr. However, inductions of necrosis and of resistance to the systemic virus movement in plants carrying Ncspl reside in different regions of the HC-Pro cistron. Also, genomic determinants outside the HC-Pro cistron are involved in the systemic movement of PVY after induction of necroses on inoculated leaves of plants carrying Nytbr. These results suggest that the Nytbr resistance may have been involved in the recent emergence of PVY isolates with a recombination breakpoint near the junction of HC-Pro and P3 cistrons in potato crops. Therefore, this emergence could constitute one of the rare examples of resistance breakdown by a virus which was caused by recombination instead of by successive accumulation of nucleotide substitutions.

scholarly journals Mutations in the Region Encoding the Central Domain of Helper Component-Proteinase (HC-Pro) Eliminate Potato Virus X/Potyviral Synergism

Virology ◽  
1997 ◽  
Vol 231 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Xing Ming Shi ◽  
Heather Miller ◽  
Jeanmarie Verchot ◽  
James C. Carrington ◽  
Vicki Bowman Vance
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Central Domain ◽  
Helper Component ◽  
Helper Component Proteinase

scholarly journals Effect of mutations within the Cys-rich region of potyvirus helper component-proteinase on self-interaction

1999 ◽  
Vol 80 (11) ◽  
pp. 2809-2812 ◽  
Author(s):  
Silvio Urcuqui-Inchima ◽  
Ivan G. Maia ◽  
Gabrièle Drugeon ◽  
Anne-Lise Haenni ◽  
Françoise Bernardi
Keyword(s):  
Amino Acids ◽  
Potato Virus Y ◽  
Yeast Two Hybrid ◽  
Rich Region ◽  
Conserved Residues ◽  
Helper Component ◽  
Yeast Two Hybrid System ◽  
Terminal Amino ◽  
Helper Component Proteinase ◽  
Two Hybrid

The first ∼60 amino acids of the N-terminal part of the potyvirus helper component-proteinase (HC-Pro) include highly conserved residues comprising a Cys-rich region. In the present study, the domain in Potato virus Y sufficient for self-interaction was mapped using the yeast two-hybrid system to the 83 N-terminal amino acids of HC-Pro. Mutations in the conserved His and two Cys residues within the Cys-rich region have a strong debilitating effect on self-interaction when introduced in the full-length HC-Pro, but not when introduced in the N-terminal fragment.

scholarly journals Characterization of a Novel Resistance-Breaking Isolate of Potato Virus Y in Nicotiana Tabacum

2014 ◽  
Vol 3 (1) ◽  
pp. 01-10
Author(s):  
Jae-Hyun Kim ◽  
Young-Soo Kim ◽  
Soo-Won Jang ◽  
Yong-Ho Jeon
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Biological Properties ◽  
Systemic Movement ◽  
Pvy Resistance ◽  
Resistance Breaking ◽  
Systemic Vein ◽  
Vein Necrosis ◽  
Tobacco Cultivar

Potato virus Y (PVY) was identified from a PVY-resistance flue-cured tobacco variety KF120 showing vein necrosis at Buron province, Korea. Biological properties of the isolate named PVY-ToBR1 was characterized using various host plants with another isolate PVY-ToJC37. The isolated PVY-ToBR1 induced systemic vein necrosis symptoms on a PVY-resistant tobacco cultivar (VAM) harboring potyvirus resistant va gene, though tissue printing showed the systemic movement of virus was slightly delayed. By contrast, the isolate PVY-ToJC37 failed to infect VAM plants and the virus was not detected on inoculated leaf and systemic leaves in VAM plants. Similarly, the isolated PVY-ToBR1 induced distinctly systemic vein necrosis symptoms on PVY-resistant tobacco cultivars (V.SCR, PBD6, TN86, TN90, Virgin A Mutant, NC744, and Wislica) that have the recessive potyvirus resistance gene va, but PVY-ToJC37 did not infect systemically infect these tobacco cultivars, suggesting that PVY-ToBR1 is a novel resistance-breaking isolate in tobacco. The coat protein (CP) genes of PVY-ToBR1 and PVY-ToJC37 were amplified using RT-PCR assays with specific primers for PVY isolates and nucleotide sequences of the CP genes were determined. The isolate PVY-ToBR1 showed 88.4% - 99.4% and 86.6% - 99.4% CP identities to the 46 different PVY isolates at the nucleotide and amino acid, respectively. Phylogenetic relationship from CP comparisons showed that PVY-ToBR1 isolate clustered with PVYNTN isolates and PVY-ToBR1 isolate more closely related to the isolates from European than from North American PVY NTN.

scholarly journals Different Mutations in the Genome-Linked Protein VPg of Potato virus Y Confer Virulence on the pvr23 Resistance in Pepper

2006 ◽  
Vol 19 (5) ◽  
pp. 557-563 ◽  
Author(s):  
Valérie Ayme ◽  
Sylvie Souche ◽  
Carole Caranta ◽  
Mireille Jacquemond ◽  
Joël Chadœuf ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genetic Drift ◽  
Potato Virus ◽  
High Frequency ◽  
Potato Virus Y ◽  
Relative Fitness ◽  
Amino Acid Substitutions ◽  
Nucleotide Substitutions ◽  
Single Nucleotide ◽  
Pepper Plants

Five different amino acid substitutions in the VPg of Potato virus Y were shown to be independently responsible for virulence toward pvr23 resistance gene of pepper. A consequence of these multiple mutations toward virulence involving single nucleotide substitutions is a particularly high frequency of resistance breaking (37% of inoculated plants from the first inoculation) and suggests a potentially low durability of pvr23 resistance. These five mutants were observed with significantly different frequencies, one of them being overrepresented. Genetic drift alone could not explain the observed distribution of virulent mutants. More plausible scenarios were obtained by taking into account either the relative substitution rates, the relative fitness of the mutants in pvr23 pepper plants, or both.

Expression of the “helper component” protein of potato virus Y (PVY) inE. coli: Possible involvement of a third protease

Virus Genes ◽  
1993 ◽  
Vol 7 (2) ◽  
pp. 151-156 ◽  
Author(s):  
Yehuda Stram ◽  
Aviva Chetsrony ◽  
Hagai Karchi ◽  
Miri Karchi ◽  
Orit Edelbaum ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Helper Component ◽  
Component Protein
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