scholarly journals The Same Allele of Translation Initiation Factor 4E Mediates Resistance Against Two Potyvirus spp. in Pisum sativum

2007 ◽  
Vol 20 (9) ◽  
pp. 1075-1082 ◽  
Author(s):  
M. Bruun-Rasmussen ◽  
I. S. Møller ◽  
G. Tulinius ◽  
J. K. R. Hansen ◽  
O. S. Lund ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Translation Initiation ◽  
Recessive Gene ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Yellow Mosaic Virus ◽  
White Lupin ◽  
Coding Region

Pathogenicity of two sequenced isolates of Bean yellow mosaic virus (BYMV) was established on genotypes of Pisum sativum L. reported to carry resistance genes to BYMV and other potyviruses. Resistance to the white lupin strain of BYMV (BYMV-W) is inherited as a recessive gene named wlv that maps to linkage group VI together with other Potyvirus resistances. One of these, sbm1, confers resistance to strains of Pea seedborne mosaic virus and previously has been identified as a mutant allele of the eukaryotic translation initiation factor 4E gene (eIF4E). Sequence comparison of eIF4E from BYMV-W-susceptible and –resistant P. sativum genotypes revealed a polymorphism correlating with the resistance profile. Expression of eIF4E from susceptible plants in resistant plants facilitated BYMV-W infection in inoculated leaves. When cDNA of BYMV-W was agroinoculated, resistance mediated by the wlv gene frequently was overcome, and virus from these plants had a codon change causing an Arg to His change at position 116 of the predicted viral genome-linked protein (VPg). Accordingly, plants carrying the wlv resistance gene were infected upon inoculation with BYMV-W derived from cDNA with a His codon at position 116 of the VPg coding region. These results suggested that VPg determined pathogenicity on plants carrying the wlv resistance gene and that wlv corresponded to the sbm1 allele of eIF4E.

scholarly journals Targeted Knockout of Eukaryotic Translation Initiation Factor 4E Confers Bymovirus Resistance in Winter Barley

2021 ◽  
Vol 3 ◽  
Author(s):  
Robert Eric Hoffie ◽  
Ingrid Otto ◽  
Dragan Perovic ◽  
Nagaveni Budhagatapalli ◽  
Antje Habekuß ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Translation Initiation ◽  
Winter Barley ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Yellow Mosaic Virus ◽  
Eukaryotic Translation Initiation Factor ◽  
Eukaryotic Translation ◽  
Barley Yellow Mosaic Virus ◽  
Eukaryotic Translation Initiation

The Eukaryotic Translation Initiation Factor 4E (EIF4E) is a well-known susceptibility factor for potyvirus infections in many plant species. The barley yellow mosaic virus disease, caused by the bymoviruses Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV), can lead to yield losses of up to 50% in winter barley. In autumn, the roots of young barley plants are infected by the soil-borne plasmodiophoraceous parasite Polymyxa graminis L. that serves as viral vector. Upon viral establishment and systemic spreading into the upper parts of the plants, yellow mosaics occur as first symptoms on leaves. In the further course of plant development, the disease entails leaf necrosis and increased susceptibility to frost damage. Thanks to the rym4 and rym5 allelic variants of the HvEIF4E gene, more than two thirds of current European winter barley cultivars are resistant to BaYMV and BaMMV. However, several strains of BaYMV and BaMMV have already overcome rym4- and rym5-mediated resistance. Accordingly, new resistance-conferring alleles are needed for barley breeding. Therefore, we performed targeted mutagenesis of the EIF4E gene by Cas9 endonuclease in BaMMV/BaYMV-susceptible winter barley cv. “Igri”. Small insertions were generated, resulting in a shift of the translational reading frame, thereby causing the loss-of-function of EIF4E. The mutations occurred in the homozygous state already in the primary mutants. Their progeny proved invariably homozygous and fully resistant to mechanical inoculation with BaMMV. EIF4E knockout plants showed normal growth habit and produced grains, yet exhibited a yield penalty.

scholarly journals Reaction of lettuce genotypes to Lettuce mosaic virus-Most (LMV-Most) and characterization of the translation factor eIF4E

2018 ◽  
Vol 53 (1) ◽  
pp. 125-129
Author(s):  
Mônika Fecury Moura ◽  
Norberto da Silva ◽  
Maria Isabel Motta Hoffmann ◽  
Marcelo Agenor Pavan ◽  
Renate Krause-Sakate
Keyword(s):  
Mosaic Virus ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Coding Region ◽  
Rt Pcr ◽  
Lettuce Mosaic Virus ◽  
Eukaryotic Translation ◽  
Translation Factor ◽  
Eukaryotic Translation Initiation

Abstract: The objective of this work was to evaluate lettuce genotypes for their reaction to Lettuce mosaic virus (LMV; Most-type, isolate AF-199) and variations of the eukaryotic translation initiation factor eIF4E. All inoculated genotypes were susceptible to LMV, which was detected by RT-PCR using specific primer pairs. However, the accessions 169501, 169501C, 172918A, and 162499 showed late development of symptoms that appeared only on the inoculated leaves. Sequencing of the coding region of eIF4E showed that these genotypes have an eIF4E0 (mol 0 ) standard typical for their susceptibility to LMV, indicating that the phenotype found is not correlated to nucleotide variations in this translation factor.

scholarly journals A DNA helicase from Pisum sativum is homologous to translation initiation factor and stimulates topoisomerase I activity

2000 ◽  
Vol 24 (2) ◽  
pp. 219-229 ◽  
Author(s):  
Xuan Hoi Pham ◽  
Malireddy Kodandarami Reddy ◽  
Nasreen Z. Ehtesham ◽  
Bharati Matta ◽  
Narendra Tuteja
Keyword(s):  
Pisum Sativum ◽  
Translation Initiation ◽  
Topoisomerase I ◽  
Dna Helicase ◽  
Translation Initiation Factor ◽  
Initiation Factor

scholarly journals The Eukaryotic Translation Initiation Factor 4E Controls Lettuce Susceptibility to the Potyvirus Lettuce mosaic virus

2003 ◽  
Vol 132 (3) ◽  
pp. 1272-1282 ◽  
Author(s):  
Valérie Nicaise ◽  
Sylvie German-Retana ◽  
Raquel Sanjuán ◽  
Marie-Pierre Dubrana ◽  
Marianne Mazier ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Translation Initiation ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Lettuce Mosaic Virus ◽  
Eukaryotic Translation Initiation Factor ◽  
Eukaryotic Translation ◽  
Eukaryotic Translation Initiation

scholarly journals Multiple Resistance Phenotypes to Lettuce mosaic virus Among Arabidopsis thaliana Accessions

2003 ◽  
Vol 16 (7) ◽  
pp. 608-616 ◽  
Author(s):  
Frédéric Revers ◽  
Thomas Guiraud ◽  
Marie-Christine Houvenaghel ◽  
Thierry Mauduit ◽  
Olivier Le Gall ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Recombinant Inbred Lines ◽  
Cell Movement ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Lettuce Mosaic Virus ◽  
Genetic Analyses ◽  
Large Variability

With the aim to characterize plant and viral factors involved in the molecular interactions between plants and potyviruses, a Lettuce mosaic virus (LMV)-Arabidopsis thaliana pathosystem was developed. Screening of Arabi-dopsis accessions with LMV isolates indicated the existence of a large variability in the outcome of the interaction, allowing the classification of Arabidopsis accessions into seven susceptibility groups. Using a reverse genetic approach, the genome-linked protein of LMV, a multifunc-tional protein shown to be involved in viral genome amplification and movement of potyviruses, was established as the viral determinant responsible for the ability to overcome the resistance of the Niederzenz accession to LMV-0. Preliminary genetic analyses from F2 and recombinant inbred lines available between susceptible and resistant Arabidopsis accessions revealed the existence of at least three resistance phenotypes to LMV with different genetic bases. One dominant resistance gene, designated LLM1, involved in blocking the replication or cell-to-cell movement of the LMV-0 isolate in the Columbia accession, was mapped to chromosome I and shown to be linked to the marker nga280. At the same time, genetic analyses of segregating F2 populations were consistent with the restriction of the systemic movement of the LMV-AF199 isolate in Columbia being controlled by two dominant genes and with the complete resistance to all tested LMV isolates of the Cape Verde islands (Cvi) accession being conferred by a single recessive resistance gene. Sequencing of the eu-karyotic translation initiation factor 4E genes from the different LMV-resistant Arabidopsis accessions showed that these factors are not directly involved in the characterized resistance phenotypes.

scholarly journals Different Haplotypes Encode the Same Protein for Independent Sources of Zucchini Yellow Mosaic Virus Resistance in Cucumber

HortScience ◽  
2017 ◽  
Vol 52 (8) ◽  
pp. 1040-1042 ◽  
Author(s):  
Axel O. Ramírez-Madera ◽  
Michael J. Havey
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Recessive Gene ◽  
Genomic Region ◽  
Yellow Mosaic Virus ◽  
Single Recessive Gene ◽  
Coding Region ◽  
Coding Regions ◽  
Genetic Uniformity ◽  
Vacuolar Protein

Cucumber (Cucumis sativus) production is negatively affected by Zucchini yellow mosaic virus (ZYMV). Three sources of ZYMV resistance have been commercially deployed and all three resistances are conditioned by a single recessive gene. A vacuolar protein sorting–associated protein 4-like (VPS4-like) gene has been proposed as a candidate for ZYMV resistance from cucumber line A192-18. We analyzed the genomic region across the VPS4-like gene for three independent sources of ZYMV resistance in cucumber (A192-18, Dina-1, and TMG-1) and identified three haplotypes across the coding region and considerable variation in the introns. However, the haplotypes in the coding regions of the VPS4-like gene of A192-18, Dina-1, and TMG-1 encode the same protein sequence, revealing the genetic uniformity for ZYMV resistance from diverse germplasm sources.

scholarly journals Bean common mosaic virus Isolate Exhibits a Novel Pathogenicity Profile in Common Bean, Overcoming the bc-3 Resistance Allele Coding for the Mutated eIF4E Translation Initiation Factor

2015 ◽  
Vol 105 (11) ◽  
pp. 1487-1495 ◽  
Author(s):  
Xue Feng ◽  
James R. Myers ◽  
Alexander V. Karasev
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Translation Initiation ◽  
Resistance Genes ◽  
Strain Analysis ◽  
Bean Common Mosaic Virus ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Resistance Allele ◽  
Eukaryotic Translation

Resistance against Bean common mosaic virus (BCMV) in Phaseolus vulgaris is governed by six recessive resistance alleles at four loci. One of these alleles, bc-3, is able to protect P. vulgaris against all BCMV strains and against other potyviruses; bc-3 was identified as the eIF4E allele carrying mutated eukaryotic translation initiation factor gene. Here, we characterized a novel BCMV isolate 1755a that was able to overcome bc-2 and bc-3 alleles in common bean. Thus, it displayed a novel pattern of interactions with resistance genes in P. vulgaris, and was assigned to a new pathogroup, PG-VIII. The IVT7214 cultivar supporting the replication of BCMV-1755a was found to have the intact homozygous bc-3 cleaved amplified polymorphic sequences marker and corresponding mutations in the eIF4E allele that confer resistance to BCMV isolates from all other pathogroups as well as to other potyviruses. The VPg protein of 1755a had seven amino acid substitutions relative to VPgs of other BCMV isolates unable to overcome bc-3. The 1755a genome was found to be a recombinant between NL1, US1 (both PG-I), and a yet unknown BCMV strain. Analysis of the recombination patterns in the genomes of NL1 and US1 (PG-I), NY15P (PG-V), US10 and RU1-OR (PG-VII), and 1755a (PG-VIII), indicated that P1/HC-Pro cistrons of BCMV strains may interact with most resistance genes. This is the first report of a BCMV isolate able to overcome the bc-3 resistance allele, suggesting that the virus has evolved mechanisms to overcome multiple resistance genes available in common bean.

scholarly journals Single Nucleotide Polymorphisms in a Gene for Translation Initiation Factor (eIF4G) of Rice (Oryza sativa) Associated with Resistance to Rice tungro spherical virus

2010 ◽  
Vol 23 (1) ◽  
pp. 29-38 ◽  
Author(s):  
Jong-Hee Lee ◽  
Muhammad Muhsin ◽  
Genelou A. Atienza ◽  
Do-Yeon Kwak ◽  
Suk-Man Kim ◽  
...  
Keyword(s):  
Translation Initiation ◽  
Recessive Gene ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Rice Tungro Bacilliform Virus ◽  
Nucleotide Polymorphisms ◽  
Rice Tungro Spherical Virus ◽  
Single Nucleotide ◽  
Rice Tungro Disease ◽  
Backcross Populations

Rice tungro disease (RTD) is a serious constraint to rice production in South and Southeast Asia. RTD is caused by Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. Rice cv. Utri Merah is resistant to RTSV. To identify the gene or genes involved in RTSV resistance, the association of genotypic and phenotypic variations for RTSV resistance was examined in backcross populations derived from Utri Merah and rice germplasm with known RTSV resistance. Genetic analysis revealed that resistance to RTSV in Utri Merah was controlled by a single recessive gene (tsv1) mapped within an approximately 200-kb region between 22.05 and 22.25 Mb of chromosome 7. A gene for putative translation initiation factor 4G (eIF4Gtsv1) was found in the tsv1 region. Comparison of eIF4Gtsv1 gene sequences among susceptible and resistant plants suggested the association of RTSV resistance with one of the single nucleotide polymorphism (SNP) sites found in exon 9 of the gene. Examination of the SNP site in the eIF4Gtsv1 gene among various rice plants resistant and susceptible to RTSV corroborated the association of SNP or deletions in codons for Val1060-1061 of the predicted eIF4Gtsv1 with RTSV resistance in rice.

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