Different Haplotypes Encode the Same Protein for Independent Sources of Zucchini Yellow Mosaic Virus Resistance in Cucumber

Cucumber (Cucumis sativus) production is negatively affected by Zucchini yellow mosaic virus (ZYMV). Three sources of ZYMV resistance have been commercially deployed and all three resistances are conditioned by a single recessive gene. A vacuolar protein sorting–associated protein 4-like (VPS4-like) gene has been proposed as a candidate for ZYMV resistance from cucumber line A192-18. We analyzed the genomic region across the VPS4-like gene for three independent sources of ZYMV resistance in cucumber (A192-18, Dina-1, and TMG-1) and identified three haplotypes across the coding region and considerable variation in the introns. However, the haplotypes in the coding regions of the VPS4-like gene of A192-18, Dina-1, and TMG-1 encode the same protein sequence, revealing the genetic uniformity for ZYMV resistance from diverse germplasm sources.

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(11) Inheritance of Resistance to Zucchini Yellow Mosaic Virus-Florida Strain in Watermelon

HortScience ◽

10.21273/hortsci.40.4.1039e ◽

2005 ◽

Vol 40 (4) ◽

pp. 1039E-1039

Author(s):

Nihat Guner ◽

Todd C. Wehner

Keyword(s):

Mosaic Virus ◽

New Hampshire ◽

Zucchini Yellow Mosaic Virus ◽

Recessive Gene ◽

Yellow Mosaic Virus ◽

Inheritance Of Resistance ◽

Single Recessive Gene ◽

Leaf Stage ◽

True Leaf ◽

Severe Isolate

Inheritance of resistance to zucchini yellow mosaic virus-Florida strain (ZYMV-FL) was studied in the resistant watermelon accession of PI 595203 (Citrulluslanatus var. lanatus), an egusi type originally collected in Nigeria. The F1, F2, and BC1 generations derived from the cross `Calhoun Gray' × PI 595203 and `New Hampshire Midget' × PI 595203 were used to study the inheritance of resistance to ZYMV-FL. Seedlings were inoculated with a severe isolate of ZYMV-FL at the first true leaf stage and rated on a 0 to 9 scale, based on the severity of virus symptoms. A single recessive gene was found to control resistance, for which we propose the symbol zym-FL2. The gene probably was not allelic to the previously published gene, zym-FL, for resistance to the Florida strain of ZYMV in the accession PI 482261, since PI 482261 was not resistant to the ZYMV-FL isolate used in our tests.

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Inheritance of Resistance to Zucchini Yellow Mosaic Virus in Watermelon

HortScience ◽

10.21273/hortsci13169-18 ◽

2018 ◽

Vol 53 (8) ◽

pp. 1115-1118 ◽

Cited By ~ 2

Author(s):

Nihat Guner ◽

Luis A. Rivera-Burgos ◽

Todd C. Wehner

Keyword(s):

Mosaic Virus ◽

New Hampshire ◽

Zucchini Yellow Mosaic Virus ◽

Recessive Gene ◽

Yellow Mosaic Virus ◽

Inheritance Of Resistance ◽

Single Recessive Gene ◽

Sources Of Resistance ◽

High Level ◽

Severe Isolate

Sources of resistance to the Zucchini yellow mosaic virus-Florida strain (ZYMV-FL) have been identified within the Citrullus genus. Inheritance of resistance to ZYMV-FL was studied in PI 595203 (Citrullus mucosospermus), a resistant watermelon accession. The F1, F2, and BC1 progenies derived from the cross ‘Calhoun Gray’ (CHG) × PI 595203 and ‘New Hampshire Midget’ (NHM) × PI 595203 were used to study the inheritance of resistance to ZYMV-FL. Seedlings were inoculated with a severe isolate of ZYMV-FL at the first true leaf stage and rated weekly for at least 6 weeks on a scale of 1 to 9 on the basis of severity of viral symptoms. A single recessive gene (zym-FL) was found to control the high level of resistance to ZYMV-FL in PI 595203.

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Inheritance of Resistance to the Moroccan Watermelon Mosaic Virus in the Cucumber Line TMG-1 and its Relationship to Zucchini Yellow Mosaic Virus Resistance

HortScience ◽

10.21273/hortsci.31.4.622e ◽

1996 ◽

Vol 31 (4) ◽

pp. 622e-622

Author(s):

Eileen Kabelka ◽

Rebecca Grumet

Keyword(s):

Mosaic Virus ◽

Single Gene ◽

Zucchini Yellow Mosaic Virus ◽

Recessive Gene ◽

Yellow Mosaic Virus ◽

Watermelon Mosaic Virus ◽

Single Recessive Gene ◽

Sequential Inoculation ◽

Distinct Member ◽

Resistant Progeny

Potyviruses cause severe loss in cucurbit crops. Inbred lines derived from the Chinese cucumber cultivar, Taichung Mau Gua (TMG), have been identified to be resistant to several potyviruses including zucchini yellow mosaic virus (ZYMV), zucchini yellow fleck virus, watermelon mosaic virus, and the watermelon strain of papaya ringspot virus. Recently, an additional virus that infects cucurbits, the Moroccan watermelon mosaic virus (MWMV), has been identified to be a distinct member of the potyvirus group. In this study, we sought to determine if TMG-1 is resistant to MWMV and, if so, examine whether a relationship exists between resistance to MWMV and resistance to ZYMV. Progeny analyses show that TMG-1 is resistant to MWMV and, like resistance to ZYMV, MWMV resistance is conferred by a single recessive gene. Sequential inoculation of progeny possessing resistance to ZYMV followed by MWMV (or MWMV followed by ZYMV) suggests that both resistances are conferred by the same gene, or two tightly linked genes. Additionally, all F3 families derived from F2 individuals selected for resistance to ZYMV, were resistant to MWMV. A second source of resistance to ZYMV, allelic to the TMG-1 source, has been incorporated into the Dutch hybrid Dina. Progeny analyses show Dina to posses a single recessive gene for MWMV resistance. As with TMG-1, no segregation of resistances was observed when ZYMV resistant progeny were inoculated with MWMV (or MWMV followed by ZYMV). Collectively, these results suggest that a single gene, or two tightly linked genes, control resistance to the potyviruses ZYMV and MWMV.

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Biological variability of zucchini yellow mosaic virus in Serbia

Pesticidi i fitomedicina ◽

10.2298/pif0904271v ◽

2009 ◽

Vol 24 (4) ◽

pp. 271-280 ◽

Cited By ~ 3

Author(s):

Ana Vucurovic ◽

Aleksandra Bulajic ◽

Ivana Djekic ◽

Danijela Ristic ◽

Janos Berenji ◽

...

Keyword(s):

Mosaic Virus ◽

Molecular Characterization ◽

Molecular Detection ◽

Zucchini Yellow Mosaic Virus ◽

Genomic Region ◽

Yellow Mosaic Virus ◽

Biological Study ◽

The Past ◽

Phenotypic Variations

Zucchini yellow mosaic virus (ZYMV) has become an important pathogen of cucurbits over the past 20 years. In our country, this virus also causes significant losses, especially in oil pumpkin production. Although ZYMV variability has been extensively studied worldwide by biological and molecular characterization, such information is still limited for the European region. Herein, we present the first analysis of phenotypic variations of ZYMV isolates sampled from oil pumpkin cv. Olinka from different locations in Serbia during 2008. Twelve isolates were obtained by mechanical inoculation on the C. pepo hybrid Ezra F1, and four of them were chosen for further detailed biological study. These investigated isolates exhibited different phenotypical features and thus represented different variants, concerning their host range, and type and severity of symptoms. A previously developed primer pair, ZYMV CPfwd/CPrev, was tested to determine its suitability for detection of Serbian ZYMV isolates. Amplification of Serbian ZYMV isolates of different origin, in single and mixed infection, resulted in one distinctive band. The used primer pair proved to be useful not only for routine molecular detection, but also for further molecular characterization of Serbian ZYMV isolates because it amplifies the hypervariable genomic region of ZYMV. The result obtained in this study is a contribution to investigation of genetic diversity of Serbian ZYMV isolates and their molecular relationship with ZYMV isolates from other parts of the world.

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The Same Allele of Translation Initiation Factor 4E Mediates Resistance Against Two Potyvirus spp. in Pisum sativum

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-20-9-1075 ◽

2007 ◽

Vol 20 (9) ◽

pp. 1075-1082 ◽

Cited By ~ 50

Author(s):

M. Bruun-Rasmussen ◽

I. S. Møller ◽

G. Tulinius ◽

J. K. R. Hansen ◽

O. S. Lund ◽

...

Keyword(s):

Pisum Sativum ◽

Mosaic Virus ◽

Resistance Gene ◽

Translation Initiation ◽

Recessive Gene ◽

Translation Initiation Factor ◽

Initiation Factor ◽

Yellow Mosaic Virus ◽

White Lupin ◽

Coding Region

Pathogenicity of two sequenced isolates of Bean yellow mosaic virus (BYMV) was established on genotypes of Pisum sativum L. reported to carry resistance genes to BYMV and other potyviruses. Resistance to the white lupin strain of BYMV (BYMV-W) is inherited as a recessive gene named wlv that maps to linkage group VI together with other Potyvirus resistances. One of these, sbm1, confers resistance to strains of Pea seedborne mosaic virus and previously has been identified as a mutant allele of the eukaryotic translation initiation factor 4E gene (eIF4E). Sequence comparison of eIF4E from BYMV-W-susceptible and –resistant P. sativum genotypes revealed a polymorphism correlating with the resistance profile. Expression of eIF4E from susceptible plants in resistant plants facilitated BYMV-W infection in inoculated leaves. When cDNA of BYMV-W was agroinoculated, resistance mediated by the wlv gene frequently was overcome, and virus from these plants had a codon change causing an Arg to His change at position 116 of the predicted viral genome-linked protein (VPg). Accordingly, plants carrying the wlv resistance gene were infected upon inoculation with BYMV-W derived from cDNA with a His codon at position 116 of the VPg coding region. These results suggested that VPg determined pathogenicity on plants carrying the wlv resistance gene and that wlv corresponded to the sbm1 allele of eIF4E.

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Inheritance of Resistance to the Florida Strain of Zucchini Yellow Mosaic Virus in Watermelon

HortScience ◽

10.21273/hortsci.26.4.407 ◽

1991 ◽

Vol 26 (4) ◽

pp. 407-408 ◽

Cited By ~ 20

Author(s):

R. Provvidenti

Keyword(s):

Mosaic Virus ◽

Citrullus Lanatus ◽

Zucchini Yellow Mosaic Virus ◽

Recessive Gene ◽

The United States ◽

Yellow Mosaic Virus ◽

Plant Selection ◽

Backcross Populations ◽

Single Plant Selection ◽

High Level

A high level of resistance to zucchini yellow mosaic virus (ZYMV) was found in four landraces of Citrullus lanatus (Thunb.) MatSum. & Nakai (PI 482322, PI 482299, PI 482261, and PI 482308) originally collected in Zimbabwe. This resistance is specific to the Florida strain of the virus (ZYMV-FL), which prevails in the United States. Inheritance studies were conducted by using a single-plant selection (PP261-I) of PI 48226I and the ZYMV-susceptible watermelon cultivar New Hampshire Midget. In F1, F2, and reciprocal backcross populations, resistance was conferred by a single recessive gene to which the symbol zym is assigned. There was no linkage between zym and one of the three homodimer bands of the isozyme phosphoglucoisomerase (Pgi-2b), which was found in PP261-1, and in other ZYMV-FL resistant plants.

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Reactions of some cucumber (Cucumis sativus L.) lines and hybrids to zucchini yellow mosaic virus (ZYMV) and selection of tolerant breeding lines

International Journal of Horticultural Science ◽

10.31421/ijhs/5/3-4/47 ◽

1999 ◽

Vol 5 (3-4) ◽

Author(s):

P. Salamon ◽

P. Balogh

Keyword(s):

Mosaic Virus ◽

Severe Disease ◽

Zucchini Yellow Mosaic Virus ◽

Disease Outbreaks ◽

Recessive Gene ◽

Yellow Mosaic Virus ◽

Pathological Effect ◽

Cucumis Sativus L ◽

Pickling Cucumber ◽

Selection Of

In the past years zucchini yellow mosaic virus (ZYMV) has been appeared as a new pathogen of cucurbitaceous plants in Hungary. It caused severe disease outbreaks on the fields of pickling cucumber hybrids which are highly tolerant to cucumber mosaic virus (CMV). Preliminary inoculation tests have showed that all of the important pickling cucumber hybrids produced in Hungary were susceptible to ZYMV-P. However, a selection of Chinese Long and the breeding line I- KS 10C proved to be highly tolerant. The Fl plants of the crosses between the tolerant lines and the susceptible genotype H I were susceptible to ZYMV-P. F2 populations segregated for susceptible and tolerant individuals at a ratio of 3:1. The results strongly suggest that the resistance to ZYMV in our sources is controlled by a single recessive gene. Inoculations of the ZYMV and CMV susceptible cultivar Budai csemege and the CMV tolerant hybrid Perez Fl with the complex of ZYMV+CMV resulted extremely severe symptoms (strong mosaic and necrotic spotting of the leaves) on both cultivars. On the Chinese Long line, which is tolerant to ZYMV and CMV, respectively, the complex of the two viruses caused mild symptoms. The results show a synergistic pathological effect of ZYMV and CMV on differrent cucumber genotypes. More detailed studies on the interactions among the plant genotypes, viruses and virus strains are needed to develop cucumber hybrids that are highly resistant to the ZYMV+CMV complex.

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Identification and Utility of Markers Linked to the Zucchini Yellow Mosaic Virus Resistance Gene in Watermelon

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.134.5.529 ◽

2009 ◽

Vol 134 (5) ◽

pp. 529-534 ◽

Cited By ~ 11

Author(s):

Karen R. Harris ◽

Kai-Shu Ling ◽

W. Patrick Wechter ◽

Amnon Levi

Keyword(s):

United States ◽

Genetic Distance ◽

Mosaic Virus ◽

Resistance Gene ◽

Zucchini Yellow Mosaic Virus ◽

Recessive Gene ◽

The United States ◽

Yellow Mosaic Virus ◽

Recurrent Parent ◽

Resistant Parent

Zucchini yellow mosaic virus (ZYMV) is one of the most economically important viruses affecting watermelon [Citrullus lanatus (Thunb.) Matsun & Nakai var. lanatus] in the United States. The ZYMV-Florida strain (ZYMV-FL) is considered a major limitation to commercial watermelon production in the United States. Inheritance of resistance to ZYMV-FL is conferred by a recessive gene. This report describes the identification of single-reaction, polymerase chain reaction-based markers linked to the ZYMV-FL resistance gene in watermelon. In this study, we identified a marker ZYMV-resistant polymorphism (ZYRP) linked to the ZYMV-FL resistance gene locus (genetic distance of 8 cM) in an F2 population, and in a backcross one to the resistant parent population (BC1R) (genetic distance of 13 cM). The identification of a single nucleotide polymorphism within the ZYRP marker for the parental genotypes allowed the development of a sequence-characterized amplification region marker linked to the ZYMV-FL resistance gene. Experiments using a BC2F2 population derived from the U.S. Plant Introduction 595203 (C. lanatus var. lanatus) and the recurrent parent ‘Charleston Gray’ indicated that the ZYRP marker can be used in marker-assisted selection to identify genotypes containing the gene conferring ZYMV-FL resistance in watermelon.

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