scholarly journals First Report of ‘Candidatus Liberibacter asiaticus’ and Its Vector Diaphorina citri in Murraya paniculata in Santiago del Estero Province, Argentina

Plant Disease ◽  
2019 ◽  
Vol 103 (5) ◽  
pp. 1014-1014
Author(s):  
G. M. Fogliata ◽  
C. V. Martínez ◽  
M. E. Acosta ◽  
A. A. Rojas ◽  
M. L. Muñoz ◽  
...  
Keyword(s):  
Diaphorina Citri ◽  
Candidatus Liberibacter Asiaticus ◽  
First Report ◽  
Murraya Paniculata ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

scholarly journals Low Incidence of ‘Candidatus Liberibacter asiaticus’ in Murraya paniculata and Associated Diaphorina citri

Plant Disease ◽  
2012 ◽  
Vol 96 (6) ◽  
pp. 827-832 ◽  
Author(s):  
Abigail J. Walter ◽  
David G. Hall ◽  
Yong Ping Duan
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Diaphorina Citri ◽  
Candidatus Liberibacter Asiaticus ◽  
Disease Epidemiology ◽  
Citrus Groves ◽  
Murraya Paniculata ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Low Incidence ◽  
A Minor

Huanglongbing (HLB) is one of the most devastating diseases of citrus worldwide. ‘Candidatus Liberibacter asiaticus’ is the prevalent species of three HLB-associated Liberibacter spp., which is vectored by the psyllid Diaphorina citri. The vector and the bacteria have host plants outside the genus Citrus, and these plants have the potential to affect disease epidemiology within citrus groves. Murraya paniculata could be especially problematic because it is a popular ornamental plant and a host of both psyllid and bacteria. We conducted a year-long survey of eight urban plantings of M. paniculata in east-central Florida to characterize ‘Ca. L. asiaticus’ infection rates in plants and associated psyllids. Using sensitive quantitative polymerase chain reaction (qPCR) primers targeting two prophage genes of ‘Ca. L. asiaticus’, we found infection to be extremely low: less than 1% of psyllids and 1.8% of plants. With qPCR primers targeting ‘Ca. L. asiaticus’ 16S rDNA, none of the plants and only one psyllid were ‘Ca. L. asiaticus’-positive. Therefore, the titer of ‘Ca. L. asiaticus’ is low in M. paniculata and associated psyllids. These results suggest that urban plantings of M. paniculata may serve as a minor source of ‘Ca. L. asiaticus’ inoculum.

scholarly journals Murraya paniculata and Swinglea glutinosa as Short-Term Transient Hosts of ‘Candidatus Liberibacter asiaticus’ and Implications for the Spread of Huanglongbing

2019 ◽  
Vol 109 (12) ◽  
pp. 2064-2073 ◽  
Author(s):  
Juan Camilo Cifuentes-Arenas ◽  
George Andrew Charles Beattie ◽  
Leandro Peña ◽  
Silvio Aparecido Lopes
Keyword(s):  
Sweet Orange ◽  
Diaphorina Citri ◽  
Candidatus Liberibacter Asiaticus ◽  
Principal Vector ◽  
Dead End ◽  
Valencia Orange ◽  
Murraya Paniculata ◽  
Citrus Rootstock ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

Murraya paniculata and Swinglea glutinosa are aurantioid hosts of the Asian citrus psyllid (ACP) Diaphorina citri, the principal vector of ‘Candidatus Liberibacter asiaticus’ (Las). Las is the pathogen associated with huanglongbing (HLB), the Asian form of which is the most devastating disease of Citrus species and cultivars (Rutaceae: Aurantioideae). M. paniculata is a common ornamental and S. glutinosa is grown as an ornamental, a citrus rootstock, and a hedgerow fence plant. Because of the uncertain status of these plants as reservoirs of Las, a series of cross-inoculation bioassays were carried out in different environments, using infected Valencia sweet orange (Citrus × aurantium) infected shoot tops as a source of inoculum and D. citri nymphs and adults reared on M. paniculata and S. glutinosa to inoculate pathogen-free Valencia orange plantlets. In contrast to sweet orange, Las was more unevenly distributed and reached much lower titers in M. paniculata and S. glutinosa. Infections in M. paniculata and S. glutinosa were also transient. Very few insects that successfully acquired Las from M. paniculata and S. glutinosa were able to transmit the pathogen to healthy citrus. Transmission rates were low from M. paniculata (1.0%) and S. glutinosa (2.0%) and occurred only in a controlled environment highly favorable to Las and ACP using 10-day-old adults that completed their life cycle on Las-positive plants. Our study showed that in HLB-endemic areas, M. paniculata and S. glutinosa can be deemed as epidemiologically dead-end hosts for Las and are not important alternative hosts of the pathogen for transmission to citrus. However, under a combination of conditions highly favorable to Las infection and transmission and in the absence of effective quarantine procedures, these plants could eventually serve as carriers of Las to regions currently free from HLB.

scholarly journals Murraya paniculata and Related Species as Potential Hosts and Inoculum Reservoirs of ‘Candidatus Liberibacter asiaticus’, Causal Agent of Huanglongbing

Plant Disease ◽  
2010 ◽  
Vol 94 (5) ◽  
pp. 528-533 ◽  
Author(s):  
V. D. Damsteegt ◽  
E. N. Postnikova ◽  
A. L. Stone ◽  
M. Kuhlmann ◽  
C. Wilson ◽  
...  
Keyword(s):  
Bacterial Disease ◽  
Diaphorina Citri ◽  
Candidatus Liberibacter Asiaticus ◽  
Infection Rates ◽  
Disease Symptoms ◽  
Murraya Paniculata ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Polymerase Chain ◽  
Southern Florida

Huanglongbing (HLB), considered to be the most serious insect-vectored bacterial disease of citrus, is transmitted in nature by the Asian citrus psyllid Diaphorina citri and the African citrus psyllid Trioza erytreae. D. citri was discovered in southern Florida in 1998 and the HLB disease in 2005. Both have become established throughout citrus-producing areas of Florida. Murraya species are widely grown in southern Florida as ornamental hedges and are readily colonized by D. citri vectors. Colonies of D. citri, isolates of ‘Candidatus Liberibacter asiaticus’ from Taiwan and Florida, and the Murraya species were established in the BSL-3 biosecurity facility at Fort Detrick. In controlled inoculation experiments, D. citri transmitted ‘Ca. L. asiaticus’ into M. paniculata (34/36 plants) and M. exotica (22/23 plants), but not into Bergera (Murraya) koenigii. Disease symptoms rarely developed in Murraya plants; however, positive infections were determined by conventional and real-time polymerase chain reaction (PCR). Back-inoculations of ‘Ca. L. asiaticus’ from M. paniculata to Madam Vinous sweet orange resulted in disease development in 25% of the inoculated plants. Considerable variability was observed in infection rates, titer, and persistence of ‘Ca. L. asiaticus’ in infected Murraya.

scholarly journals IDENTIFICACIÓN Y EVALUACIÓN DE LA INCIDENCIA DE INSECTOS Y HONGOS BENÉFICOS ASOCIADOS A Diaphorina citri KUWAYAMA (HEMIPTERA: LIVIIDAE) EN PLANTAS TRASPATIO (Citrus spp. y Murraya paniculata) DEL CANTÓN CATAMAYO (LOJA - ECUADOR)

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Marlon Erráez Aguilera ◽  
Marina Mazón ◽  
Henry Troya Armijos ◽  
Doris Valarezo Espinoza
Keyword(s):  
Diaphorina Citri ◽  
Candidatus Liberibacter Asiaticus ◽  
Murraya Paniculata ◽  
Candidatus Liberibacter ◽  
Alternaria Sp ◽  
Liberibacter Asiaticus ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Citrus Spp

En los últimos años, la citricultura mundial se ha encontrado amenazada por la presencia del psílido asiático de los cítricos, Diaphorina citri, un insecto perjudicial por ser portador de la bacteria “Candidatus Liberibacter asiaticus (las)”, causante de la enfermedad Huanglongbing (HLB), que ha provocado la devastación de plantaciones y la reducción de la producción de cítricos en todo el mundo. En el año 2017 se reportó la presencia de D. citri en plantas traspatio del cantón Catamayo, en la provincia de Loja, por lo que la presente investigación tuvo por objetivo identificar los enemigos naturales (depredadores, parasitoides y hongos) asociados a D. citri, y evaluar su incidencia en campo. Para ello, se realizaron muestreos en plantas traspatio (Citrus spp. y Murraya paniculata) del cantón Catamayo. Los muestreos se efectuaron a una altura de 1,5 m de las plantas en los cuatro puntos cardinales de la misma. Se colectaron larvas y adultos de depredadores, ninfas parasitadas y adultos de parasitoides, y adultos de D. citri para el aislamiento de hongos. Como resultado de la identificación en el laboratorio, se determinó la presencia de 11 especies depredadoras (principalmente Cheilomenes sexmaculata y Chrysopa spp.), dos especies de parasitoides (Diaphorencyrtus sp. y Tamarixia radiata) y cuatro especies de hongos (Cladosporium sp., Fusarium sp., Aspergillus sp. y Alternaria sp.). Diaphorencyrtus sp. tuvo una tasa de parasitismo del 7,76 %. Estas especies podrían utilizarse para establecer programas de control biológico, aprovechando su potencial como reguladores de poblaciones de ninfas de Diaphorina citri.

scholarly journals First report of ‘Candidatus Liberibacter asiaticus’ in Diaphorina communis

2011 ◽  
Vol 7 (1) ◽  
pp. 1-4 ◽  
Author(s):  
N. J. Donovan ◽  
G. A. C. Beattie ◽  
G. A. Chambers ◽  
P. Holford ◽  
A. Englezou ◽  
...  
Keyword(s):  
Candidatus Liberibacter Asiaticus ◽  
First Report ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

Probing the application of OmpA-derived peptides to disrupt the acquisition of ‘Candidatus Liberibacter asiaticus’ by Diaphorina citri

2021 ◽  
Author(s):  
Marcus Vinicius Merfa e Silva ◽  
Eduarda Regina Fischer ◽  
Mariana de Souza e Silva ◽  
Carolina Sardinha Francisco ◽  
Helvécio Coletta-Filho ◽  
...  
Keyword(s):  
Control Strategies ◽  
Insect Vector ◽  
Diaphorina Citri ◽  
Candidatus Liberibacter Asiaticus ◽  
In Planta ◽  
Host Interaction ◽  
Vector Population ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Bacterial Outer Membrane

Huanglongbing (HLB) is currently the most devastating disease of citrus worldwide. Both bacteria ‘Candidatus Liberibacter asiaticus’ (CLas) and ‘Ca. Liberibacter americanus’ (CLam) are associated with HLB in Brazil, but with a strong prevalence of CLas over CLam. Conventionally, HLB management focuses on controlling the insect vector population (Diaphorina citri; also known as Asian citrus psyllid – ACP) by spraying insecticides, an approach demonstrated to be mostly ineffective. Thus, development of novel more efficient HLB control strategies is required. The multifunctional bacterial outer membrane protein OmpA is involved in several molecular processes between bacteria and their hosts and has been suggested as a target for bacterial control. Curiously, OmpA is absent in CLam in comparison to CLas, suggesting a possible role on host-interaction. Therefore, in the current study, we have treated ACPs with different OmpA-derived peptides aiming to evaluate the acquisition of CLas by the insect vector. Treatment of psyllids with 5 µM of Pep1, Pep3, Pep5 and Pep6 in artificial diet significantly reduced the acquisition of CLas, while increasing the concentration of Pep5 and Pep6 to 50 µM abolished this process. In addition, in planta treatment with 50 µM of Pep6 also significantly decreased the acquisition of CLas and sweet orange plants stably absorbed and maintained this peptide for as long as three months post the final application. Together, our results demonstrate the promising use of OmpA-derived peptides as a novel biotechnological tool to control CLas.

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