Colonization of Wild Blackberry Plants in California by Fusarium oxysporum f. sp. mori

Plant Disease ◽  
2021 ◽  
pp. PDIS-06-20-1297
Author(s):  
Ana M. Pastrana ◽  
Dean C. Watson ◽  
Thomas R. Gordon
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Elongation Factor ◽  
Fruit Production ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Commercial Cultivars ◽  
Rubus Armeniacus ◽  
Rubus Species ◽  
Elongation Factor 1

Fusarium oxysporum f. sp. mori, the causal agent of Fusarium wilt of blackberry, was first reported in California and Mexico in 2016. A limited survey of the population revealed this pathogen to be one of the most diverse formae speciales of F. oxysporum. We explored the possibility that strains of F. oxysporum pathogenic to commercial blackberry could also be recovered from wild blackberry (Rubus spp.) in California. For this purpose, wild Rubus species in blackberry nurseries, fruit production fields, and nearby areas were collected between 2017 and 2019. Thirty-four isolates of F. oxysporum were recovered from asymptomatic Rubus armeniacus and Rubus ursinus plants. Based on sequence of the translation elongation factor 1-α, somatic compatibility, and pathogenicity to blackberry, 16 isolates were confirmed as F. oxysporum f. sp. mori. These isolates were associated with three somatic compatibility groups, one of which was first identified in this study. Recovery of the pathogen confirmed that wild blackberry plants can act as a reservoir of inoculum of F. oxysporum f. sp. mori and that it can move from wild blackberry plants to commercial cultivars or vice versa.

scholarly journals Differences in Pathogenicity, Genetic Variability and Cultivar Responses among Isolates of Fusarium oxysporum from Strawberry in Australia

2016 ◽  
Vol 141 (6) ◽  
pp. 645-652 ◽  
Author(s):  
Michelle L. Paynter ◽  
Elizabeth Czislowski ◽  
Mark E. Herrington ◽  
Elizabeth A.B. Aitken
Keyword(s):  
Fusarium Oxysporum ◽  
Elongation Factor ◽  
Fragaria Ananassa ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Vegetative Compatibility Groups ◽  
Elongation Factor 1 Alpha ◽  
Pathogenicity Tests ◽  
Cultivar Responses ◽  
Elongation Factor 1

Variation in the virulence of Fusarium oxysporum f. sp. fragariae (Fof) strains is important when evaluating the resistance of plants to this fungus. Twenty-five isolates of F. oxysporum harvested from strawberry (Fragaria ×ananassa) plants growing in Australia were characterized using pathogenicity tests, vegetative compatibility groups (VCGs), and genetic analysis of translation elongation factor 1 alpha (EF-1α). The level of disease varied depending on isolate used, indicating heterogeneous populations of Fof. Two distinct VCGs were identified and corresponded to two of the 10 lineages identified by partial EF-1α. Using a subset of Fof isolates, resistance in eight cultivars ranged from highly resistant to highly susceptible, with some cultivar × isolate interaction. ‘Strawberry Festival’, ‘QHI Sugarbaby’, and ‘DPI Rubygem’ had high levels of resistance across all isolates. Isolates from Western Australia (WA) were genetically distinct from those from Queensland (QLD) and were more virulent to ‘Camarosa’, a major cultivar grown in WA.

Sequence of a cDNA encoding the α-subunit of wheat translation elongation factor 1

Gene ◽  
1992 ◽  
Vol 120 (2) ◽  
pp. 315-316 ◽  
Author(s):  
Anneke M. Metz ◽  
Richard T. Timmer ◽  
M.Leah Allen ◽  
Karen S. Browning
Keyword(s):  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Α Subunit ◽  
Elongation Factor 1

scholarly journals Global Molecular Diversity of the Halotolerant Fungus Hortaea werneckii

Life ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 31 ◽  
Author(s):  
Alessia Marchetta ◽  
Bert Gerrits van den Ende ◽  
Abdullah Al-Hatmi ◽  
Ferry Hagen ◽  
Polona Zalar ◽  
...  
Keyword(s):  
Molecular Diversity ◽  
Sea Water ◽  
Elongation Factor ◽  
Clonal Expansion ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Hortaea Werneckii ◽  
Halotolerant Fungus ◽  
Elongation Factor 1 ◽  
Partial Translation

A global set of clinical and environmental strains of the halotolerant black yeast-like fungus Hortaea werneckii are analyzed by multilocus sequencing and AFLP, and physiological parameters are determined. Partial translation elongation factor 1-α proves to be suitable for typing because of the presence/absence of introns and also the presence of several SNPs. Local clonal expansion could be established by a combination of molecular methods, while the population from the Mediterranean Sea water also responds differently to combined temperature and salt stress. The species comprises molecular populations, which in part also differ physiologically allowing further diversification, but clinical strains did not deviate significantly from their environmental counterparts.

Diaporthe sinensis, a new fungus from Amaranthus sp. in China 

Phytotaxa ◽  
2019 ◽  
Vol 425 (5) ◽  
pp. 259-268
Author(s):  
XIAO-XIAO FENG ◽  
JIA-JIE CHEN ◽  
GUO-RONG WANG ◽  
TING-TING CAO ◽  
YONG-LI ZHENG ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Plant Pathogens ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Fungal Species ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Stems And Leaves ◽  
Elongation Factor 1 ◽  
Β Tubulin

During an exploration of plant pathogens in vegetables occuring in Zhejiang province, China, a novel fungal species, was found. Three strains ZJUP0033-4, ZJUP0038-3 and ZJUP0132 were isolated from black round lesions in the stems and leaves of Amaranthus sp. Phylogenetic analyses based on sequences from four genes including rDNA internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α), histone (HIS) and β-tubulin (TUB) indicated that D. sinensis clustered in a distinct clade closely related to D. neoarctii, D. angelicae, D. subordinaria, D. arctii, D. cuppatea, D. lusitanicae, D. novem, D. infecunda, D. ganjae and D. manihotia. Morphologically, D. sinensis is distinguished by brown, scattered, globose pycnidia and ellipsoid alpha conidia with bi- to multiguttulate.

scholarly journals Fusarium oxysporumf. sp.mori, a New Forma Specialis Causing Fusarium Wilt of Blackberry

Plant Disease ◽  
2017 ◽  
Vol 101 (12) ◽  
pp. 2066-2072 ◽  
Author(s):  
A. M. Pastrana ◽  
S. C. Kirkpatrick ◽  
M. Kong ◽  
J. C. Broome ◽  
T. R. Gordon
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Elongation Factor ◽  
Intergenic Spacer ◽  
Translation Elongation ◽  
Data Set ◽  
Tubulin Genes ◽  
Forma Specialis ◽  
Sequence Types ◽  
Strawberry Cultivars

Fusarium oxysporum has recently been identified as the cause of a wilt disease affecting blackberry in California and Mexico. Thirty-six isolates of F. oxysporum obtained from symptomatic blackberry plants in California and Mexico were comprised of nine distinct somatic compatibility groups (SCGs). Phylogenetic analysis of a concatenated data set, consisting of sequences of the translation elongation factor 1-α and β-tubulin genes and the intergenic spacer of the ribosomal DNA, identified nine three-locus sequence types, each of which corresponded to an SCG. Six SCGs were present only in California, two only in Mexico, and one in both California and Mexico. An isolate associated with the most common SCG in California was tested for pathogenicity on blueberry, raspberry, strawberry, and lettuce. All blueberry, raspberry, and lettuce plants that were inoculated remained healthy, but two of the five strawberry cultivars tested developed symptoms. The three strawberry cultivars that were resistant to the blackberry pathogen were also resistant to F. oxysporum f. sp. fragariae, the cause of Fusarium wilt of strawberry. We propose to designate strains of F. oxysporum that are pathogenic to blackberry as Fusarium oxysporum f. sp. mori forma specialis nov.

Additions to pestalotioid fungi in Thailand: Neopestalotiopsis hydeana sp. nov. and Pestalotiopsis hydei sp. nov.

Phytotaxa ◽  
2021 ◽  
Vol 479 (1) ◽  
pp. 23-43
Author(s):  
NARUEMON HUANALUEK ◽  
RUVISHIKA S. JAYAWARDENA ◽  
SAJEEWA S. N. MAHARACHCHIKUMBURA ◽  
DULANJALEE L. HARISHCHANDRA
Keyword(s):  
Elongation Factor ◽  
Climatic Conditions ◽  
Annona Squamosa ◽  
Translation Elongation Factor ◽  
Garcinia Mangostana ◽  
Translation Elongation ◽  
Leaf Spots ◽  
First Time ◽  
Elongation Factor 1 ◽  
Partial Translation

Pestalotioid fungi commonly occur as pathogens, endophytes or saprobes. In this study, pestalotioid fungi associated with leaf spots and fruit rots were isolated from Alpinia malaccensis, A. galangal, Annona squamosa, Artocarpus heterophyllus, Citrus sp., Garcinia mangostana, Litsea petiolata, a pteridophyte, and Vitis vinifera in Chiang Rai, Thailand. Based on single- and multi-locus phylogenies using internal transcribed spacer, β-tubulin and partial translation elongation factor 1-α gene regions, along with morphological features, the isolates were identified as two new species, Neopestalotiopsis hydeana and Pestalotiopsis hydei. This is the first time a Pestalotiopsis sp. was reported associated with Litsea petiolata and a Neopestalotiopsis sp. recorded from Alpinia, Annona, Artocarpus, Garcinia and a pteridophyte in the world. This fungal group can be considered as an emerging pathogenic group on different hosts in different climatic conditions. 

scholarly journals Translation elongation factor 1-α gene as a potential taxonomic and identification marker in dermatophytes

2014 ◽  
Vol 53 (3) ◽  
pp. 215-224 ◽  
Author(s):  
Hossein Mirhendi ◽  
Koichi Makimura ◽  
G. Sybren de Hoog ◽  
Ali Rezaei-Matehkolaei ◽  
Mohammad Javad Najafzadeh ◽  
...  
Keyword(s):  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1
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