Diaporthe sinensis, a new fungus from Amaranthus sp. in China

During an exploration of plant pathogens in vegetables occuring in Zhejiang province, China, a novel fungal species, was found. Three strains ZJUP0033-4, ZJUP0038-3 and ZJUP0132 were isolated from black round lesions in the stems and leaves of Amaranthus sp. Phylogenetic analyses based on sequences from four genes including rDNA internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α), histone (HIS) and β-tubulin (TUB) indicated that D. sinensis clustered in a distinct clade closely related to D. neoarctii, D. angelicae, D. subordinaria, D. arctii, D. cuppatea, D. lusitanicae, D. novem, D. infecunda, D. ganjae and D. manihotia. Morphologically, D. sinensis is distinguished by brown, scattered, globose pycnidia and ellipsoid alpha conidia with bi- to multiguttulate.

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Additions to Karst Fungi 5: Sardiniella guizhouensis sp. nov. (Botryosphaeriaceae) associated with woody hosts in Guizhou province, China

Phytotaxa ◽

10.11646/phytotaxa.508.2.7 ◽

2021 ◽

Vol 508 (2) ◽

Author(s):

YA-YA CHEN ◽

ASHA J. DISSANAYAKE ◽

JIAN-KUI LIU

Keyword(s):

Internal Transcribed Spacer ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Guizhou Province ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Distinct Lineage ◽

Elongation Factor 1 ◽

A New Species

During an investigation of ascomycetous fungi in Karst formations of the Asian region, three interesting taxa were found on dead aerial stems of woody hosts in Guizhou province, China. Their morphology assigned them as typical botryosphaeriaceous species. Phylogenetic analyses based on a combined DNA dataset of large subunit (LSU), internal transcribed spacer (ITS) and part of the translation elongation factor 1-α (tef1) gene confirmed their placement within Botryosphaeriaceae. In the phylogenetic tree, the three isolates formed a well-supported monotypic clade as a distinct lineage within the genus Sardiniella. Therefore, a new species Sardiniella guizhouensis sp. nov. is introduced to accommodate these taxa and detailed, illustrated descriptions of the asexual and sexual morphs are provided. This study reveals the first sexual morph of Sardiniella, which it is characterized by 2–4(–6)-spored asci with hyaline to brown, aseptate to 1-septate, ovate to subclavate ascospores.

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Pestalotiopsis anacardiacearum sp. nov. (Amphisphaeriaceae) has an intricate relationship with Penicillaria jocosatrix the mango tip borer

Phytotaxa ◽

10.11646/phytotaxa.99.2.1 ◽

2013 ◽

Vol 99 (2) ◽

pp. 49 ◽

Cited By ~ 10

Author(s):

SAJEEWA S. N. MAHARACHCHIKUMBURA ◽

YANMIN ZHANG ◽

YONG WANG ◽

KEVIN D. HYDE

Keyword(s):

Internal Transcribed Spacer ◽

Yunnan Province ◽

Mangifera Indica ◽

Elongation Factor ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Elongation Factor 1 Alpha ◽

Elongation Factor 1 ◽

Partial Translation ◽

Β Tubulin

Pestalotiopsis anacardiacearum sp. nov. is described from leaves of Mangifera indica from Yunnan Province, China. The taxon can clearly be distinguished from all known species of Pestalotiopsis by its morphology. Phylogenetic analysis based on combined multi-locus alignment of the internal transcribed spacer (ITS), partial β-tubulin and partial translation elongation factor 1-alpha (tef1) also distinguishes this taxon. It can be distinguished from previously recorded Pestalotiopsis pathogens on mango by having larger conidia. The species occurs on leaves of mango following death associated with the mango tip borer (Penicillaria jocosatrix).

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Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China

MycoKeys ◽

10.3897/mycokeys.80.63816 ◽

2021 ◽

Vol 80 ◽

pp. 77-90

Author(s):

Xianhong Wang ◽

Yashuang Guo ◽

Yamin Du ◽

Ziling Yang ◽

Xinzhong Huang ◽

...

Keyword(s):

Internal Transcribed Spacer ◽

Novel Species ◽

Phylogenetic Analyses ◽

Elongation Factor ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Wide Range ◽

Elongation Factor 1

Species of Diaporthe infect a wide range of plants and live in vivo as endophytes, saprobes or pathogens. However, those in peach plants are poorly characterized. In this study, 52 Diaporthe strains were isolated from peach branches with buds, showing constriction canker symptoms. Phylogenetic analyses were conducted using five gene regions: internal transcribed spacer of the ribosomal DNA (ITS), translation elongation factor 1-α (TEF), ß-tubulin (TUB), histone (HIS), and calmodulin (CAL). These results coupled with morphology revealed seven species of Diaporthe, including five known species (D. caryae, D. cercidis, D. eres, D. hongkongensis, and D. unshiuensis). In addition, two novel species D. jinxiu and D. zaofenghuang are introduced. Except for the previously reported D. eres, this study represents the first characterization of Diaporthe species associated with peach constriction canker in China, and contributes useful data for practicable disease management.

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Clonostachys viticola sp. nov., a novel species isolated from Vitis vinifera

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijsem.0.004286 ◽

2020 ◽

Vol 70 (7) ◽

pp. 4321-4328

Author(s):

Cristina Torcato ◽

Micael F. M. Gonçalves ◽

Edgar Rodríguez-Gálvez ◽

Artur Alves

Keyword(s):

Internal Transcribed Spacer ◽

Novel Species ◽

Phylogenetic Analyses ◽

Elongation Factor ◽

The Other ◽

Translation Elongation Factor ◽

Sequence Analyses ◽

Translation Elongation ◽

Elongation Factor 1 Alpha ◽

Elongation Factor 1

A collection of fungal isolates obtained from crop plants, specifically grapevine and blueberry, in Peru were characterised through morphological and DNA sequence analyses of the nuclear ribosomal internal transcribed spacer (ITS), beta-tubulin (tub2) and translation elongation factor 1-alpha (tef-1α) regions. Isolates produced monomorphic and dimorphic conidiophores typical of members of the genus Clonostachys. Single- and multi-locus gene phylogenies confirmed the isolates as representing members of the genus Clonostachys, more closely related to species in the subgenus Bionectria. In phylogenetic analyses the isolates grouped in two separate clades, one corresponding to the species Clonostachys pseudochroleuca and the other one distinct from all known species of the genus Clonostachys. These isolates are recognized as representing a novel species species for which the name Clonostachys viticola is proposed.

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New Endophytic Fusarium spp. from Fraxinus excelsior Leaves in Slovakia

Planta Medica ◽

10.1055/a-1197-3613 ◽

2020 ◽

Vol 86 (13/14) ◽

pp. 1025-1031

Author(s):

Helena Ivanova ◽

Lenka Malinicova ◽

Maria Piknova ◽

Peter Pristas

Keyword(s):

Internal Transcribed Spacer ◽

Elongation Factor ◽

Fraxinus Excelsior ◽

Translation Elongation Factor ◽

Fusarium Spp ◽

Translation Elongation ◽

Ash Dieback ◽

Elongation Factor 1 Alpha ◽

Elongation Factor 1 ◽

Β Tubulin

Abstract Fraxinus excelsior is currently suffering from ash dieback disease caused by the fungus Hymenoscyphus fraxineus. Co-occurrence of large numbers of other fungi with endophytic as well as pathogenic lifestyles has been observed on F. excelsior. In this study, new endophytic Fusarium spp. isolate Fraxinus excelsior Fusarium from Slovak territory, associated with F. excelsior, was characterised. Using morphology-based approaches, the fungus was assigned to the genus Fusarium. The isolate formed thick-walled hyaline, fusiform, slightly curved macroconidia, produced from monophialides in sporodochia, pointed at the tip, mostly 3 – 5 septate, occasionally 6 septate, and 40 – 68 × 3.8 – 5.0 µm in size with basal pedicellate cells. For more accurate taxonomic affiliation, molecular sequence analyses of the internal transcribed spacer, translation elongation factor 1-alpha sequences, and partial β-tubulin gene were performed. While the internal transcribed spacer sequence of the isolate showed the highest similarity to the Fraxinus oxysporum species, translation elongation factor 1-alpha and partial β-tubulin sequences were distantly related to the Fraxinus avenaceum species. Based on these data, the Fraxinus excelsior Fusarium isolate could probably be considered a new species of the Fusarium genus.

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Trichoderma polyalthiae sp. nov., an endophytic fungus from Polyalthia debilis

Phytotaxa ◽

10.11646/phytotaxa.371.5.1 ◽

2018 ◽

Vol 371 (5) ◽

pp. 273 ◽

Cited By ~ 2

Author(s):

KATESARAPORN NUANKAEW ◽

KOZUE SOTOME ◽

SAISAMORN LUMYONG ◽

SOPHON BOONLUE

Keyword(s):

Internal Transcribed Spacer ◽

Phylogenetic Analyses ◽

Elongation Factor ◽

Translation Elongation Factor ◽

Phenotypic Characteristics ◽

Internal Transcribed Spacer Region ◽

Translation Elongation ◽

Closely Related Species ◽

Elongation Factor 1 ◽

A New Species

A new species of endophytic fungi, Trichoderma polyalthiae, was isolated from stems of Polyalthia debilis in Ubon Ratchatani Province, Thailand. Trichoderma polyalthiae was distinguished from closely related species by its molecular and phenotypic characteristics. Based on multigene phylogenetic analyses of the internal transcribed spacer region, translation elongation factor 1-α, and RNA polymerase subunit II gene sequences, T. polyalthiae formed a distinct clade. Morphologically, T. polyalthiae can be distinguished from closely related taxa by its growth rates, diffusing of pigment into agar, and hyaline conidia and production of chlamydospores.

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Phylogenetic analyses of the Fusarium poae, Fusarium sporotrichioides and Fusarium langsethiae species complex based on partial sequences of the translation elongation factor-1 alpha gene

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2003.12.007 ◽

2004 ◽

Vol 95 (3) ◽

pp. 287-295 ◽

Cited By ~ 57

Author(s):

A.K. Knutsen ◽

M. Torp ◽

A. Holst-Jensen

Keyword(s):

Species Complex ◽

Phylogenetic Analyses ◽

Elongation Factor ◽

Fusarium Sporotrichioides ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Elongation Factor 1 Alpha ◽

Fusarium Poae ◽

Alpha Gene ◽

Elongation Factor 1

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A taxonomic and phylogenetic re-appraisal of the genus Curvularia (Pleosporaceae): human and plant pathogens

Phytotaxa ◽

10.11646/phytotaxa.212.3.1 ◽

2015 ◽

Vol 212 (3) ◽

pp. 175 ◽

Cited By ~ 32

Author(s):

DIMUTHU SANDARENU MANAMGODA ◽

AMY Y. ROSSMAN ◽

LISA A. CASTLEBURY ◽

EKACHAI CHUKEATIROTE ◽

KEVIN HYDE

Keyword(s):

Internal Transcribed Spacer ◽

Plant Material ◽

Plant Pathogens ◽

Elongation Factor ◽

Opportunistic Pathogens ◽

Translation Elongation Factor ◽

Translation Elongation ◽

The World ◽

Novel Host ◽

Associated Species

Curvularia is an important genus whose species are widely distributed phytopathogens as well as opportunistic pathogens on human and animals. The purpose of this study is to re-evaluate the phylogenetic relationships of the species in the genus Curvularia using ITS (nuclear ribosomal internal transcribed spacer), GPDH (glyceraldehyde-3-phosphate dehydrogenase) and TEF (translation elongation factor) gene regions and to provide modern descriptions and illustrations of Curvularia australis, Curvularia buchloës, C. cymbopogonis, C. hawaiiensis, C. neoindica, C. neergaardii, C. nicotiae, C. nodulosa, C. ryleyi, and C. subpapendorfii which lack recent descriptions with details of host and distribution. A multi-gene phylogenetic tree based on ITS, GPDH and TEF gene regions is used to define species of a fresh collections obtained from various hosts and geographic locations in the world. Both human and plant associated species of Curvularia are included in the phylogenetic analysis. Some species that have previously been described from humans are herein reported from plant material as pathogens or saprobes and vice versa. Novel host associations are reported for C. asianensis, C. borreriae, C. hominis, C. muehlenbeckiae, C. trifolii and C. verruculosa.

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Phylogeny and morphology reveal two new species of Diaporthe from Betula spp. in China

Phytotaxa ◽

10.11646/phytotaxa.269.2.2 ◽

2016 ◽

Vol 269 (2) ◽

pp. 90 ◽

Cited By ~ 11

Author(s):

ZHUO DU ◽

XIN-LEI FAN ◽

KEVIN D. HYDE ◽

QIN YANG ◽

YING-MEI LIANG ◽

...

Keyword(s):

Internal Transcribed Spacer ◽

Sequence Data ◽

Histone H3 ◽

Elongation Factor ◽

Betula Platyphylla ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Wide Range ◽

Dieback Disease ◽

Elongation Factor 1

Diaporthe species are common pathogens, endophytes, or saprobes on a wide range of hosts. During our investigation of forest pathogens, we made collections of Diaporthe species associated with canker and dieback disease of Betula platyphylla and B. albosinensis in Sichuan and Shaanxi provinces in China. Diaporthe betulae sp. nov. and D. betulicola sp. nov. are introduced in this paper, with illustrations, descriptions and support from analysis of ribosomal DNA internal transcribed spacer (ITS), calmodulin (CAL), histone H3 (HIS), translation elongation factor 1-α (TEF1-α) and beta-tubulin (TUB2) sequence data. Diaporthe betulae is characterized by hyaline, ellipsoidal, aseptate, biguttulate, 8.5–11 × 3–4 µm alpha conidia. Diaporthe betulicola is characterized by pycnidial stromata with a single locule with one ostiole per disc. Alpha conidia are hyaline, oblong, aseptate, lack guttules and 9.9–14.7 × 1.3–2.5 µm, and beta conidia are hyaline, spindle-shaped, curved, aseptate and 17–24 × 0.7–1.2 µm.

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