scholarly journals Reduction of Botrytis cinerea Colonization of and Sporulation on Bunch Trash

Plant Disease ◽  
2020 ◽  
Vol 104 (3) ◽  
pp. 808-816 ◽  
Author(s):  
Giorgia Fedele ◽  
Elisa González-Domínguez ◽  
Melissa Si Ammour ◽  
Luca Languasco ◽  
Vittorio Rossi

Botrytis bunch rot (BBR) of grapevine, caused by Botrytis cinerea, is commonly managed by fungicide (FUN) sprays at flowering (A), at prebunch closure (B), at veraison (C), and before harvest. Applications at A, B, and C are recommended to reduce B. cinerea colonization of bunch trash and the production of conidia during berry ripening. The effects of these applications were previously evaluated as reductions in BBR severity at harvest rather than as reductions in bunch trash colonization and sporulation by B. cinerea. This study investigated the effects of FUNs (a commercial mixture of fludioxonil and cyprodonil), biological control agents (BCAs; Aureobasium pullulans and Trichoderma atroviride), and botanicals (BOTs; a commercial mixture of eugenol, geraniol, and thymol) applied at different timings (A, B, C, or ABC) compared with a nontreated control (NT) on B. cinerea bunch trash colonization and sporulation in vineyards. The ability of B. cinerea to colonize the bunch trash (as indicated by B. cinerea DNA content) and sporulate (as indicated by the number of conidia produced under optimal laboratory conditions) was highly variable, and this variability was higher between years (2015 to 2018) than among the three vineyards and three sampling times (i.e., 1 week after applications at A, B, and C). B. cinerea sporulation on bunch trash was significantly lower in plots treated with FUN than in NT in only 3 of 18 cases (3 vineyards × 2 years × 3 sampling times). FUN applications, however, significantly reduced B. cinerea colonization of bunch trash compared with NT; for colonization, BCA efficacy was similar to that of FUN, but BOT efficacy was variable. For all products, colonization reduction was the same with application at A versus ABC, meaning that the effect of an early season application lasted from flowering to 1 week after veraison. These results indicate that the early season control of B. cinerea is important to reduce the saprophytic colonization of bunch trash, especially when the risk of BBR is high.

2013 ◽  
Vol 70 (4) ◽  
pp. 595-602 ◽  
Author(s):  
Carlos Calvo-Garrido ◽  
Inmaculada Viñas ◽  
Philip AG Elmer ◽  
Josep Usall ◽  
Neus Teixidó

2019 ◽  
Vol 116 (39) ◽  
pp. 19743-19752 ◽  
Author(s):  
Aline Voxeur ◽  
Olivier Habrylo ◽  
Stéphanie Guénin ◽  
Fabien Miart ◽  
Marie-Christine Soulié ◽  
...  

Despite an ever-increasing interest for the use of pectin-derived oligogalacturonides (OGs) as biological control agents in agriculture, very little information exists—mainly for technical reasons—on the nature and activity of the OGs that accumulate during pathogen infection. Here we developed a sensitive OG profiling method, which revealed unsuspected features of the OGs generated during infection of Arabidopsis thaliana with the fungus Botrytis cinerea. Indeed, in contrast to previous reports, most OGs were acetyl- and methylesterified, and 80% of them were produced by fungal pectin lyases, not by polygalacturonases. Polygalacturonase products did not accumulate as larger size OGs but were converted into oxidized GalA dimers. Finally, the comparison of the OGs and transcriptomes of leaves infected with B. cinerea mutants with reduced pectinolytic activity but with decreased or increased virulence, respectively, identified candidate OG elicitors. In conclusion, OG analysis provides insights into the enzymatic arms race between plant and pathogen and facilitates the identification of defense elicitors.


2016 ◽  
Vol 69 ◽  
pp. 78-85 ◽  
Author(s):  
N. Cripps-Guazzone ◽  
E.E. Jones ◽  
L.M. Condron ◽  
K.L. McLean ◽  
A. Stewart ◽  
...  

The colonisation of plant roots by biological control agents is dependent on abiotic factors one of the most important being soil pH The rhizosphere and endophytic colonisation of ryegrass and sweet corn roots by the biological control agent Trichoderma atroviride LU132 were assessed in a pot experiment with nonsterile soil at three different pHs (55 65 and 75) T atroviride LU132 colonised the roots of both plants regardless of the soil pH with 113147 x 106 CFU/g of dry rhizosphere soil (DRS) for ryegrass and 136350x105 CFU/g DRS for sweet corn T atroviride LU132 was able to colonise both plants endophytically regardless of soil pH However the isolate was recovered from entire ryegrass roots but only from the upper parts of sweet corn roots These experiments demonstrated that T atroviride LU132 colonised the rhizosphere and roots within a soil pH range common to most NZ soils which is a desirable trait for biological control agents


BioControl ◽  
2010 ◽  
Vol 56 (3) ◽  
pp. 353-363 ◽  
Author(s):  
S. Ajouz ◽  
A. S. Walker ◽  
F. Fabre ◽  
P. Leroux ◽  
P. C. Nicot ◽  
...  

2013 ◽  
Vol 103 (10) ◽  
pp. 1001-1011 ◽  
Author(s):  
Justine Sylla ◽  
Beatrix W. Alsanius ◽  
Erika Krüger ◽  
Annette Reineke ◽  
Stephan Strohmeier ◽  
...  

The increasing use of biological control agents (BCAs) against Botrytis cinerea in strawberry raises the question of whether there are any undesirable impacts of foliar applications of BCAs on nontarget microorganisms in the phyllosphere. Therefore, our objective was to investigate this issue within a field study. Strawberry plants were repeatedly sprayed with three BCAs—namely, RhizoVital 42 fl. (Bacillus amyloliquefaciens FZB42), Trianum-P (Trichoderma harzianum T22), and Naturalis (Beauveria bassiana ATCC 74040)—to suppress Botrytis cinerea infections. Microbial communities of differentially treated leaves were analyzed using plate counts and pyrosequencing and compared with the microbial community of nontreated leaves. Plate count results indicate that the applied Bacillus and Trichoderma spp. survived in the strawberry phyllosphere throughout the strawberry season. However, no significant impacts on the leaf microbiota could be detected by this culture-dependent technique. Pyrosequencing of internal transcribed spacer ribosomal RNA and 16S RNA sequences revealed a change in fungal composition and diversity at class level after the introduction of T. harzianum T22 to the phyllosphere, whereas the bacterial composition and diversity was not affected by either this Trichoderma preparation or the other two BCAs. Our results suggest that pyrosequencing represents a useful method for studying microbial interactions in the phyllosphere.


EDIS ◽  
2017 ◽  
Vol 2017 (6) ◽  
Author(s):  
James P. Cuda ◽  
Patricia Prade ◽  
Carey R. Minteer-Killian

In the late 1970s, Brazilian peppertree, Schinus terebinthifolia Raddi (Sapindales: Anacardiaceae), was targeted for classical biological control in Florida because its invasive properties (see Host Plants) are consistent with escape from natural enemies (Williams 1954), and there are no native Schinus spp. in North America. The lack of native close relatives should minimize the risk of damage to non-target plants from introduced biological control agents (Pemberton 2000). [...]


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