During late July and early August of 2010, a serious disease of peanut (Arachis hypogaea L.) resembling Cylindrocladium black rot (CBR) was found in Longyan City, Fujian Province of Eastern China. Aboveground symptoms were chlorosis and yellowing of leaves, a black rot of the basal stem and pegs, and wilting of the vines. Underground plant parts (including pods, pegs, taproots, and hypocotyls) were blackened and rotted. Orange-to-reddish spherical fruiting bodies appeared on the lesions of the basal stems and pegs of peanut. Disease incidence was approximately 20%. A fungus was consistently isolated from the edge of lesions on potato dextrose agar (PDA) amended with streptomycin and incubated at 25°C. The fungus produced white-to-pale buff mycelia with a yellowish brown pigment. Optimum growth of the fungus on PDA was at 25 to 30°C. Conidiophores were borne laterally on a stipe that terminated in a hyaline, globose vesicle measuring 5.5 × 10.9 μm in diameter. Conidia were hyaline, cylindrical, rounded at both ends, slightly wider at the base than at the apex, with one to three septa (mostly one septa), and measured 27.3 to 70.9 × 4.1 to 8.2 μm. Orange-to-reddish perithecia were readily formed in old cultures. The perithecia were subglobose to oval or obovate and measured 215.6 to 609.4 × 309.4 to 496.9 μm. The asci were hyaline, clavate, thin walled, long stalked, with each containing eight ascospores. Ascospores were hyaline, falcate, had one septum, and measured 27.3 to 54.5 × 4.1 to 6.8 μm. The fungus was identified as Cylindrocladium parasiticum Crous, M.J. Wingfield, & Alfenas (teleomorph Calonectria ilicicola) (1,2). The beta-tubulin gene fragment was amplified using the T1/Bt2b primers (3) and sequenced. The sequences of three isolates (GenBank Accession Nos. JF343965, JF429656, and JF429657), when compared with existing sequences in GenBank, had 95 to 99% sequence identity with Calonectria ilicicola (GenBank Accession Nos. AY725643 and AY725639). Pathogenicity tests were conducted by first culturing the fungus on wheat kernels for 2 weeks. Inoculated kernels were then used as inoculum and mixed with sterilized soil in a proportion of 1:20 by weight in plastic pots (10 × 9 cm). Noninoculated wheat kernels were mixed with sterilized soil in the same proportion and served as the control. Two-week-old peanut seedlings (cv. Yueyou No. 7) were transplanted into inoculated or noninoculated pots. There were five plants per pot and each treatment was replicated four times. The plants were incubated in a greenhouse at 25 ± 2°C. All of the treated plants exhibited typical basal stem and root rot symptoms of CBR 2 weeks after inoculation, while all of the control plants remained healthy. C. parasiticum was reisolated from the diseased plants. To our knowledge, this is the first report of CBR on peanut in Fujian Province in Eastern China. The disease has been previously reported in Guangdong Province in Southern China but is not known elsewhere (4). This pathogen may pose a serious threat to peanut production in China, where peanut is an important crop. References: (1) D. K. Bell and E. K. Sobers. Phytopathology 56:1361, 1966. (2) P. W. Crous et al. Mycol. Res. 97:889, 1993. (3) P. W. Crous et al. Can. J. Bot. 77:1813, 1999. (4) R. Pan et al. Plant Pathol. 58:1176, 2009.
First Report of Black Rot of Medicago sativa Caused by Cylindrocladium parasiticum (teleomorph Calonectria ilicicola) in Yunnan Province, China
