scholarly journals Biological and Molecular Characterization of a Novel Tobamovirus with a Unique Host Range

Plant Disease ◽  
2003 ◽  
Vol 87 (10) ◽  
pp. 1190-1196 ◽  
Author(s):  
Scott Adkins ◽  
Ivanka Kamenova ◽  
Diann Achor ◽  
Dennis J. Lewandowski
Keyword(s):  
Coat Protein ◽  
Host Range ◽  
Protein Sequence ◽  
Genome Organization ◽  
Protein Gene ◽  
Coat Protein Sequence ◽  
Plant Viruses ◽  
Experimental Host Range ◽  
Solanaceous Plants

Tobamoviruses are among the best characterized and most studied plant viruses. Three subgroups of tobamoviruses correspond to viral genome sequence and host range to include those viruses infecting (i) solanaceous plants, (ii) brassicas, or (iii) cucurbits or legumes. We isolated a virus from Florida landscape plantings of the malvaceous plant hibiscus (Hibiscus rosasinensis) that appears to be a tobamovirus based upon its virion morphology, genome organization, and coat protein sequence. The experimental host range of this virus included five malvaceous species but excluded all tested brassica, cucurbit, and legume species and 12 of the 19 solanaceous species tested. The unique host range and comparison of coat protein gene and protein sequences with those of recognized tobamoviruses indicate that this is a novel to-bamovirus. A limited survey revealed that this virus is widespread in hibiscus and related species in the Florida landscape.

scholarly journals Preliminary molecular characterization of some Citrus tristeza Closterovirus isolates infecting Croatian citrus

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 264-266
Author(s):  
S. Černi ◽  
D. Škorić ◽  
M. Krajačić
Keyword(s):  
Coat Protein ◽  
Protein Sequence ◽  
Coastal Region ◽  
Coat Protein Sequence ◽  
Rt Pcr ◽  
Double Stranded Rna ◽  
The World ◽  
Citrus Tristeza Closterovirus ◽  
Citrus Tristeza

Citrus tristeza Closterovirus (CTV) is widespread in major citrus-growing regions of the world often causing destructive diseases. Citrus samples were taken from orchards in the Croatian coastal region. CTV was detected in two symptomless field trees of Satsuma mandarins and one diseased lemon tree. Double-stranded RNA was isolated from the field trees and the dsRNA patterns were compared in polyacrylamide gels. The same dsRNA extracts were used as templates in RT-PCR experiments amplifying the CTV coat protein sequence. Amplicons were subjected to SSCP and RFLP analyses. The results indicate greater similarity between CTV isolates from Satsuma mandarins than between these two and the lemon isolate.

Molecular detection and coat protein gene based characterization of Citrus tristeza virus prevalent in Sikkim state of India

2019 ◽  
Vol 73 (1) ◽  
pp. 135-143 ◽  
Author(s):  
Ashish Warghane ◽  
Amol Kokane ◽  
Sunil Kokane ◽  
Manali Motghare ◽  
Datta Surwase ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Molecular Detection ◽  
Citrus Tristeza Virus ◽  
Protein Gene ◽  
Tristeza Virus ◽  
Citrus Tristeza

Preliminary x-ray characterization of authentic providence virus and attempts to express its coat protein gene in recombinant baculovirus

2005 ◽  
Vol 151 (1) ◽  
pp. 155-165 ◽  
Author(s):  
D. J. Taylor ◽  
J. A. Speir ◽  
V. Reddy ◽  
G. Cingolani ◽  
F. M. Pringle ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Recombinant Baculovirus ◽  
X Ray
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