scholarly journals The G143A Mutation Confers Azoxystrobin Resistance to Soybean Cercospora Leaf Blight in Bolivia

2019 ◽  
Vol 20 (1) ◽  
pp. 2-3 ◽  
Author(s):  
Francisco J. Sautua ◽  
Jacob Searight ◽  
Vinson P. Doyle ◽  
Paul P. Price ◽  
Maria M. Scandiani ◽  
...  
Keyword(s):  
South America ◽  
Cytochrome B ◽  
Elongation Factor ◽  
Leaf Blight ◽  
First Report ◽  
Histone 3 ◽  
Elongation Factor 1 Alpha ◽  
Soybean Leaves ◽  
High Level ◽  
Elongation Factor 1

During the summer of 2017, 38 Cercospora spp. isolates were collected from soybean leaves displaying symptoms of Cercospora leaf blight (CLB) from commercial soybean fields at three locations in Santa Cruz, Bolivia. Portions of cytochrome b (N = 38) and calmodulin (N = 37) were amplified, sequenced, edited, and assembled. Two representative isolates from each lineage (with distinct calmodulin haplotypes, if present) were selected for sequencing of two additional loci, histone 3 and elongation factor 1-alpha, for species identification. Based on the corresponding multilocus phylogenetic analysis, the 37 isolates belong to three species: C. nicotianae (N = 14), C. sp. “P” (N = 16), and C. kikuchii (N = 7). All 38 isolates (100%) possessed the G143A mutation in cytochrome b, and none carried the F129L mutation. Results of phenotypic assays on a subset of 15 isolates (five from each species) supported a high level of resistance to azoxystrobin. This is the first report of the G143A mutation in Cercospora species associated with CLB in South America and the first report of C. nicotianae associated with CLB. Future monitoring for G143A mutants of Cercospora spp. in South America will be necessary to assess whether strobilurin resistance is widespread.

scholarly journals First report of Fusarium solani causing stem rot of Dracaena in Iran

2016 ◽  
Vol 56 (1) ◽  
pp. 100-103 ◽  
Author(s):  
Mostafa Abedi-Tizaki ◽  
Doustmorad Zafari ◽  
Jamal Sadeghi
Keyword(s):  
Fusarium Solani ◽  
Elongation Factor ◽  
Stem Rot ◽  
Pathogenicity Test ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
First Report ◽  
Brown Discoloration ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

Abstract In July 2013, symptoms of stem rot were observed in the Dracaena sanderiana cuttings in greenhouses of Mahallat County, Markazi Province, Iran. The symptoms first appeared as severe wilting. Later, leaves became brown and necrotic. Symptoms on the cuttings were observed as rotted areas on the middle of the stems. The cortical tissues of the plants showed a distinct brown discoloration. Eventually, the infected plants died. The pathogen was isolated from Dracaena stems and identified as F. solani by a fragment of the translation elongation factor 1-alpha (EF-1α) gene. Fusarium solani was confirmed by a pathogenicity test, and the causal agent was re-isolated from infected D. sanderiana plants. To the best of our knowledge, this is the first report of stem rot caused by F. solani on the cuttings of D. sanderiana.

scholarly journals Cloning, nucleotide sequence, and expression of one of two genes coding for yeast elongation factor 1 alpha.

1985 ◽  
Vol 260 (5) ◽  
pp. 3090-3096
Author(s):  
P Cottrelle ◽  
D Thiele ◽  
V L Price ◽  
S Memet ◽  
J Y Micouin ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Elongation Factor ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

scholarly journals A higher plant extracellular vitronectin-like adhesion protein is related to the translational elongation factor-1 alpha.

1994 ◽  
Vol 6 (3) ◽  
pp. 393-404 ◽  
Author(s):  
J K Zhu ◽  
B Damsz ◽  
A K Kononowicz ◽  
R A Bressan ◽  
P M Hasegawa
Keyword(s):  
Elongation Factor ◽  
Higher Plant ◽  
Adhesion Protein ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

Amanita griseofusca: A new species of Amanita in section Vaginatae from Malam Jabba, Swat, Pakistan

Phytotaxa ◽  
2018 ◽  
Vol 364 (2) ◽  
pp. 181 ◽  
Author(s):  
MUNAZZA KIRAN ◽  
JUNAID KHAN ◽  
HASSAN SHER ◽  
DONALD H. PFISTER ◽  
ABDUL NASIR KHALID
Keyword(s):  
New Species ◽  
Elongation Factor ◽  
Molecular Data ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Pileus Surface ◽  
Elongation Factor 1 ◽  
A New Species ◽  
Partial Translation

A new species, Amanita griseofusca in section Vaginatae is described and illustrated here from Pakistan. Distinguishing characters of the new species include medium-sized basidiomata, greyish brown pileus surface with white to beige, membranous volval remnants present as one (large) to a few (small) warts, close lamellae which are cream colored with a pink tone, striations one third of the total pileus radius, broadly ellipsoidal to ellipsoidal basidiospores and white loose saccate volva turning beige at maturity. Molecular data inferred from partial nuc rDNA internal transcribed spacer region (ITS), partial nuc rDNA larger subunit region (LSU) and partial translation elongation factor 1-alpha (tef1) confirms the novelty of the present taxon.

scholarly journals First report of Gibellulopsis nigrescens on peppermint in Germany

2021 ◽  
Author(s):  
Elias Alisaac ◽  
Monika Götz
Keyword(s):  
Verticillium Wilt ◽  
Elongation Factor ◽  
Economic Losses ◽  
Fungal Culture ◽  
Translation Elongation ◽  
First Report ◽  
Regular Control ◽  
Fungal Dna ◽  
Elongation Factor 1 ◽  
Important Medicinal Plant

AbstractPeppermint is an important medicinal plant, and it is known for its essential oils and phenolic acids. Verticillium wilt is a vascular disease resulted from several Verticillium spp. causing significant economic losses in peppermint cultivation. In this study, the fungus Gibellulopsis nigrescens (syn. Verticillium nigrescens) was isolated from symptomless peppermint plants during the regular control of Verticillium wilt on peppermint in Germany. A pure fungal culture was prepared, and fungal DNA was extracted. Ribosomal internal transcribed spacer (ITS), beta-tubulin (TUB), and translation elongation factor 1-α (TEF1-α) were amplified, sequenced, and deposited in the GenBank. These sequences are located within the Gibellulopsis nigrescens cluster. Koch’s postulate was fulfilled, and the fungus was re-isolated from the inoculated plants. Up to our knowledge, this is the first report of Gibellulopsis nigrescens on peppermint in Germany.

A new genus, Perplexacara, and new generic placements of species of Australian marsh beetles (Coleoptera: Scirtidae) based on morphology and molecular genetic data

Zootaxa ◽  
2021 ◽  
Vol 4927 (4) ◽  
pp. 539-548
Author(s):  
C.H.S. WATTS ◽  
T.M. BRADFORD ◽  
S.J.B. COOPER
Keyword(s):  
New Genus ◽  
Sequence Data ◽  
Mitochondrial Gene ◽  
Molecular Genetic ◽  
Elongation Factor ◽  
New Combination ◽  
Cytochrome Oxidase Subunit ◽  
Molecular Genetic Data ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

The Australian Scirtidae species previously identified as misplaced in the widespread genus Prionocyphon Redtenbacher are revisited as well as their possible relationship with the Australian genus Macrodascillus (Lea) using sequence data from the mitochondrial gene, cytochrome oxidase subunit 1 and two nuclear genes, elongation factor 1-alpha and Topoisomerase. The study confirmed the conclusion of Cooper et al. (2014) that the species did not belong in Prionocyphon. The study also included a species from each of three possibly related genera, Chameloscyphon Watts, Daploeuros Watts and Dasyscyphon Watts. Chameloscyphon huonensis Watts, Dasyscyphon victoriaensis Watts and Daploeuros lamingtonensis Watts were recovered as separate lineages with C. huonensis linking with Das. victoriaensis and Dap. lamingtonensis isolated. The species previously included in Prionocyphon were shown to belong in two genera, Macrodascillus and a new genus Perplexacara: Perplexacara caementum (Watts) new combination, P. latusmandibulara (Watts) new combination, P. macroflavida (Watts) new combination, Macrodascillus scalaris (Lea), M. insolitus (Watts) new combination and M. lamingtonensis (Watts) new combination. 

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