scholarly journals First report of Gibellulopsis nigrescens on peppermint in Germany

2021 ◽  
Author(s):  
Elias Alisaac ◽  
Monika Götz
Keyword(s):  
Verticillium Wilt ◽  
Elongation Factor ◽  
Economic Losses ◽  
Fungal Culture ◽  
Translation Elongation ◽  
First Report ◽  
Regular Control ◽  
Fungal Dna ◽  
Elongation Factor 1 ◽  
Important Medicinal Plant

AbstractPeppermint is an important medicinal plant, and it is known for its essential oils and phenolic acids. Verticillium wilt is a vascular disease resulted from several Verticillium spp. causing significant economic losses in peppermint cultivation. In this study, the fungus Gibellulopsis nigrescens (syn. Verticillium nigrescens) was isolated from symptomless peppermint plants during the regular control of Verticillium wilt on peppermint in Germany. A pure fungal culture was prepared, and fungal DNA was extracted. Ribosomal internal transcribed spacer (ITS), beta-tubulin (TUB), and translation elongation factor 1-α (TEF1-α) were amplified, sequenced, and deposited in the GenBank. These sequences are located within the Gibellulopsis nigrescens cluster. Koch’s postulate was fulfilled, and the fungus was re-isolated from the inoculated plants. Up to our knowledge, this is the first report of Gibellulopsis nigrescens on peppermint in Germany.

scholarly journals First report of Fusarium solani causing stem rot of Dracaena in Iran

2016 ◽  
Vol 56 (1) ◽  
pp. 100-103 ◽  
Author(s):  
Mostafa Abedi-Tizaki ◽  
Doustmorad Zafari ◽  
Jamal Sadeghi
Keyword(s):  
Fusarium Solani ◽  
Elongation Factor ◽  
Stem Rot ◽  
Pathogenicity Test ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
First Report ◽  
Brown Discoloration ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

Abstract In July 2013, symptoms of stem rot were observed in the Dracaena sanderiana cuttings in greenhouses of Mahallat County, Markazi Province, Iran. The symptoms first appeared as severe wilting. Later, leaves became brown and necrotic. Symptoms on the cuttings were observed as rotted areas on the middle of the stems. The cortical tissues of the plants showed a distinct brown discoloration. Eventually, the infected plants died. The pathogen was isolated from Dracaena stems and identified as F. solani by a fragment of the translation elongation factor 1-alpha (EF-1α) gene. Fusarium solani was confirmed by a pathogenicity test, and the causal agent was re-isolated from infected D. sanderiana plants. To the best of our knowledge, this is the first report of stem rot caused by F. solani on the cuttings of D. sanderiana.

Amanita griseofusca: A new species of Amanita in section Vaginatae from Malam Jabba, Swat, Pakistan

Phytotaxa ◽  
2018 ◽  
Vol 364 (2) ◽  
pp. 181 ◽  
Author(s):  
MUNAZZA KIRAN ◽  
JUNAID KHAN ◽  
HASSAN SHER ◽  
DONALD H. PFISTER ◽  
ABDUL NASIR KHALID
Keyword(s):  
New Species ◽  
Elongation Factor ◽  
Molecular Data ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Pileus Surface ◽  
Elongation Factor 1 ◽  
A New Species ◽  
Partial Translation

A new species, Amanita griseofusca in section Vaginatae is described and illustrated here from Pakistan. Distinguishing characters of the new species include medium-sized basidiomata, greyish brown pileus surface with white to beige, membranous volval remnants present as one (large) to a few (small) warts, close lamellae which are cream colored with a pink tone, striations one third of the total pileus radius, broadly ellipsoidal to ellipsoidal basidiospores and white loose saccate volva turning beige at maturity. Molecular data inferred from partial nuc rDNA internal transcribed spacer region (ITS), partial nuc rDNA larger subunit region (LSU) and partial translation elongation factor 1-alpha (tef1) confirms the novelty of the present taxon.

scholarly journals Survey, Identification, and Characterization of Cylindrocarpon-Like Asexual Morphs in Spanish Forest Nurseries

Plant Disease ◽  
2018 ◽  
Vol 102 (11) ◽  
pp. 2083-2100 ◽  
Author(s):  
Beatriz Mora-Sala ◽  
Ana Cabral ◽  
Maela León ◽  
Carlos Agustí-Brisach ◽  
Josep Armengol ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Elongation Factor ◽  
Its Region ◽  
Phylogenetic Position ◽  
Translation Elongation ◽  
Forest Nurseries ◽  
Asexual Morphs ◽  
Identification And Characterization ◽  
Elongation Factor 1

Cylindrocarpon-like asexual morphs infect herbaceous and woody plants, mainly in agricultural scenarios, but also in forestry systems. The aim of the present study was to characterize a collection of Cylindrocarpon-like isolates recovered from the roots of a broad range of forest hosts from nurseries showing decline by morphological and molecular studies. Between 2009 and 2012, 17 forest nurseries in Spain were surveyed and a total of 103 Cylindrocarpon-like isolates were obtained. Isolates were identified based on DNA sequences of the partial gene regions histone H3 (his3). For the new species, the internal transcribed spacer and intervening 5.8S nrRNA gene (ITS) region, β-tubulin (tub2), and translation elongation factor 1-α (tef1) were also used to determine their phylogenetic position. Twelve species belonging to the genera Cylindrodendrum, Dactylonectria, and Ilyonectria were identified from damaged roots of 15 different host genera. The species C. alicantinum, D. macrodidyma, D. novozelandica, D. pauciseptata, D. pinicola, D. torresensis, I. capensis, I. cyclaminicola, I. liriodendri, I. pseudodestructans, I. robusta, and I. rufa were identified. In addition, two Dactylonectria species (D. hispanica sp. nov. and D. valentina sp. nov.), one Ilyonectria species (I. ilicicola sp. nov.), and one Neonectria species (N. quercicola sp. nov.) are newly described. The present study demonstrates the prevalence of this fungal group associated with seedlings of diverse hosts showing decline symptoms in forest nurseries in Spain.

Characterization and pathogenicity of Diplodia, Lasiodiplodia and Neofusicoccum species causing Botryosphaeria canker and dieback of apple trees in central Chile

Plant Disease ◽  
2021 ◽  
Author(s):  
Gonzalo A. Díaz ◽  
Adrián Vinicio Valdez ◽  
Francois Halleen ◽  
Enrique Ferrada ◽  
Mauricio A. Lolas ◽  
...  
Keyword(s):  
Disease Incidence ◽  
Elongation Factor ◽  
Its Region ◽  
Morphological Identification ◽  
Apple Trees ◽  
Translation Elongation ◽  
Central Chile ◽  
Growing Seasons ◽  
First Time ◽  
Elongation Factor 1

In recent years, the number of apple trees affected by Botryosphaeria cankers and dieback has considerably increased in central Chile. This study aimed to identify the species of Botryosphaeriaceae associated with canker and dieback symptoms, estimate disease incidence and distributions, and study their pathogenicity and virulence on apple and other fruit crops. A field survey of 34 commercial orchards of apple (7-to 30-year-old) was conducted in 16 localities obtaining 270 symptomatic branches and trunks samples in 2017 and 2018 growing seasons. The incidence of Botryosphaeria canker and dieback ranged between 5 and 40%, and a total of 255 isolates of Botryosphaeriaceae spp. were obtained from 238 cankers. Morphological identification along with phylogenetics studies of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) of the rDNA, part of the translation elongation factor 1-α (tef1-α), and part of the beta tubulin (tub2) genes allowed to identify Diplodia mutila (n = 49 isolates), D. seriata (n = 136 isolates), Lasiodiplodia theobromae (n = 16 isolates) and Neofusicoccum arbuti (n= 54 isolates). L. theobromae was isolated mainly of apple dieback from northern localities. All pathogens tested were pathogenic, causing canker and dieback symptoms on lignified twigs of apple, pear, walnut, and green grapevine shoots in the field. Isolates of N. arbuti were the most virulent by reproducing more severe cankers on lignified tissues inoculated. This study reports D. mutila and L. theobromae for the first time associated with Botryosphaeria canker and dieback in Chile, and it is the first description of N. arbuti causing apple dieback worldwide.

Sequence of a cDNA encoding the α-subunit of wheat translation elongation factor 1

Gene ◽  
1992 ◽  
Vol 120 (2) ◽  
pp. 315-316 ◽  
Author(s):  
Anneke M. Metz ◽  
Richard T. Timmer ◽  
M.Leah Allen ◽  
Karen S. Browning
Keyword(s):  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Α Subunit ◽  
Elongation Factor 1

A new species of Scleroramularia associated with sooty blotch and flyspeck in Southern China

Phytotaxa ◽  
2015 ◽  
Vol 226 (3) ◽  
pp. 261 ◽  
Author(s):  
Liu Gao ◽  
Zhuoya Wei ◽  
Bo Wang ◽  
Mark L. Gleason ◽  
Rong Zhang ◽  
...  
Keyword(s):  
New Species ◽  
Southern China ◽  
Elongation Factor ◽  
Space Region ◽  
Translation Elongation ◽  
Sooty Blotch ◽  
Elongation Factor 1 Alpha ◽  
Sooty Blotch And Flyspeck ◽  
Elongation Factor 1 ◽  
A New Species

Scleroramularia is a genus that includes species of the sooty blotch and flyspeck (SBFS) fungal complex that blemishes the surface of apple, pawpaw, and other fruit. In a survey of SBFS on banana (Musa basjoo), an isolate associated with the flyspeck mycelial type of SBFS was obtained from Zhanjiang, Guangdong, China. Based on analysis of morphology and phylogeny (the nuclear ribosomal internal transcribed space region and translation elongation factor 1-alpha), it was delimited as a new species of Scleroramularia, described here as S. musae. Conidia of S. musae have more septa than other species presently known in the genus.

Morphological characters and phylogenetic analysis reveal a new species of Phellinus with hooked hymenial setae from Vietnam

Phytotaxa ◽  
2018 ◽  
Vol 356 (1) ◽  
pp. 91 ◽  
Author(s):  
LIN ZHU ◽  
XING JI ◽  
JING SI ◽  
BAO-KAI CUI
Keyword(s):  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Molecular Data ◽  
Translation Elongation ◽  
The Status ◽  
Large Subunit Rdna ◽  
Elongation Factor 1 ◽  
A New Species

Phellinus vietnamensis sp. nov. is described from Vietnam based on morphological characters and molecular data. Morphologically, it is characterized by perennial, pileate basidiomata, a dimitic hyphal system, hooked hymenial setae, and colorless, broadly subglobose to ovoid, thick-walled basidiospores 5.5–6 × 4.8–5.2 μm. Phylogenetically, the status of Phellinus vietnamensis is strongly supported based on sequences of the nuclear internal transcribed spacer (ITS) regions, the translation elongation factor 1-α gene (EF1-α) nuclear large subunit rDNA (nrLSU) and the second largest subunits of RNA polymerase II (RPB2).

scholarly journals Global Molecular Diversity of the Halotolerant Fungus Hortaea werneckii

Life ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 31 ◽  
Author(s):  
Alessia Marchetta ◽  
Bert Gerrits van den Ende ◽  
Abdullah Al-Hatmi ◽  
Ferry Hagen ◽  
Polona Zalar ◽  
...  
Keyword(s):  
Molecular Diversity ◽  
Sea Water ◽  
Elongation Factor ◽  
Clonal Expansion ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Hortaea Werneckii ◽  
Halotolerant Fungus ◽  
Elongation Factor 1 ◽  
Partial Translation

A global set of clinical and environmental strains of the halotolerant black yeast-like fungus Hortaea werneckii are analyzed by multilocus sequencing and AFLP, and physiological parameters are determined. Partial translation elongation factor 1-α proves to be suitable for typing because of the presence/absence of introns and also the presence of several SNPs. Local clonal expansion could be established by a combination of molecular methods, while the population from the Mediterranean Sea water also responds differently to combined temperature and salt stress. The species comprises molecular populations, which in part also differ physiologically allowing further diversification, but clinical strains did not deviate significantly from their environmental counterparts.

scholarly journals Bambusicolous Arthrinium Species in Guangdong Province, China

2020 ◽  
Vol 11 ◽  
Author(s):  
Indunil C. Senanayake ◽  
Jayarama D. Bhat ◽  
Ratchadawan Cheewangkoon ◽  
Ning Xie
Keyword(s):  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Guangdong Province ◽  
Nuclear Ribosomal Dna ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Bambusicolous Fungi ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

A survey of bambusicolous fungi in Bijiashan Mountain Park, Shenzhen, Guangdong Province, China, revealed several Arthrinium-like taxa from dead sheaths, twigs, and clumps of Bambusa species. Phylogenetic relationships were investigated based on morphology and combined analyses of the internal transcribed spacer region (ITS), large subunit nuclear ribosomal DNA (LSU), beta tubulin (β-tubulin), and translation elongation factor 1-alpha (tef 1-α) gene sequences. Based on morphological characteristics and phylogenetic data, Arthrinium acutiapicum sp. nov. and Arthrinium pseudorasikravindrae sp. nov. are introduced herein with descriptions and illustrations. Additionally, two new locality records of Arthrinium bambusae and Arthrinium guizhouense are described and illustrated.

A new species of Heimioporus (Boletaceae) from southern China

Phytotaxa ◽  
2019 ◽  
Vol 415 (4) ◽  
pp. 179-188
Author(s):  
XIANG-NYU CHEN ◽  
MING ZHANG ◽  
TAI-HUI LI ◽  
NIAN-KAI ZENG
Keyword(s):  
New Species ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Molecular Data ◽  
Translation Elongation ◽  
Distinct Member ◽  
Elongation Factor 1 ◽  
A New Species

Heimioporus sinensis, collected from tropical and subtropical areas of China, is introduced as a new species based on both morphological characters and molecular data. The species is characterized by the purplish red to deep magenta pileus, the reticulated stipe, the irregularly reticulate to reticulate-alveolate basidiospores 11.5–13.5 × 8–9.5 μm, and a trichodermal to intricately trichodermal pileipellis. Phylogenetic analyses based on the nuc 28S rDNA D1-D2 domains (28S) and the translation elongation factor 1-α gene (tef1-α) showed that H. sinensis is a distinct member of the genus Heimioporus in the subfamily Xerocomoideae.

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