Identification of a New Cotton Disease Caused by an Atypical Cotton Leafroll Dwarf Virus in Argentina

An outbreak of a new disease occurred in cotton (Gossypium hirsutum) fields in northwest Argentina starting in the 2009–10 growing season and is still spreading steadily. The characteristic symptoms of the disease included slight leaf rolling and a bushy phenotype in the upper part of the plant. In this study, we determined the complete nucleotide sequences of two independent virus genomes isolated from cotton blue disease (CBD)-resistant and -susceptible cotton varieties. This virus genome comprised 5,866 nucleotides with an organization similar to that of the genus Polerovirus and was closely related to cotton leafroll dwarf virus, with protein identity ranging from 88 to 98%. The virus was subsequently transmitted to a CBD-resistant cotton variety using Aphis gossypii and symptoms were successfully reproduced. To study the persistence of the virus, we analyzed symptomatic plants from CBD-resistant varieties from different cotton-growing fields between 2013 and 2015 and showed the presence of the same virus strain. In addition, a constructed full-length infectious cDNA clone from the virus caused disease symptoms in systemic leaves of CBD-resistant cotton plants. Altogether, the new leafroll disease in CBD-resistant cotton plants is caused by an atypical cotton leafroll dwarf virus.

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Inheritance of resistance to cotton blue disease

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2008000500015 ◽

2008 ◽

Vol 43 (5) ◽

pp. 661-665 ◽

Cited By ~ 9

Author(s):

Osmério Pupim Junior ◽

Ivan Schuster ◽

Ronald Barth Pinto ◽

Ely Pires ◽

Jean-Louis Belot ◽

...

Keyword(s):

Disease Resistance ◽

Dominant Gene ◽

Artificial Inoculation ◽

Inheritance Of Resistance ◽

Greenhouse Test ◽

Resistant Varieties ◽

Cotton Blue ◽

Cotton Plants ◽

Cotton Blue Disease

The objective of this work was to determine the inheritance of cotton blue disease resistance by cotton plants. Populations derived from the CD 401 and Delta Opal resistant varieties were evaluated, through a greenhouse test with artificial inoculation by viruliferous aphids. Cotton blue disease resistance is conditioned by one dominant gene, both in CD 401 and Delta Opal varieties.

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First Report of Cotton leafroll dwarf virus Associated with Cotton Blue Disease Symptoms in Alabama

Plant Disease ◽

10.1094/pdis-09-18-1550-pdn ◽

2019 ◽

Vol 103 (3) ◽

pp. 592-592 ◽

Cited By ~ 11

Author(s):

S. Avelar ◽

D. W. Schrimsher ◽

K. Lawrence ◽

J. K. Brown

Keyword(s):

Dwarf Virus ◽

Disease Symptoms ◽

First Report ◽

Cotton Blue ◽

Cotton Blue Disease ◽

Cotton Leafroll Dwarf Virus

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The complete genome sequence of a virus associated with cotton blue disease, cotton leafroll dwarf virus, confirms that it is a new member of the genus Polerovirus

Archives of Virology ◽

10.1007/s00705-010-0764-3 ◽

2010 ◽

Vol 155 (11) ◽

pp. 1849-1854 ◽

Cited By ~ 26

Author(s):

Ana J. Distéfano ◽

Ivan Bonacic Kresic ◽

H. Esteban Hopp

Keyword(s):

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Dwarf Virus ◽

Cotton Blue ◽

Cotton Blue Disease ◽

Cotton Leafroll Dwarf Virus

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First Report of Cotton Leafroll Dwarf Virus in Louisiana

Plant Health Progress ◽

10.1094/php-03-20-0019-br ◽

2020 ◽

Vol 21 (2) ◽

pp. 142-143 ◽

Cited By ~ 1

Author(s):

Trey Price ◽

Rodrigo Valverde ◽

Raghuwinder Singh ◽

Jeff Davis ◽

Sebe Brown ◽

...

Keyword(s):

United States ◽

Molecular Diagnosis ◽

Dwarf Virus ◽

Southern United States ◽

Symptom Development ◽

Cotton Aphid ◽

Aphid Infestation ◽

First Report ◽

Cotton Plants ◽

Cotton Leafroll Dwarf Virus

Cotton leafroll dwarf virus (CLRDV) has recently been discovered in the southern United States. The virus is transmitted by the cotton aphid and causes numerous symptoms including foliar chlorosis, distortion, leaf cupping, and reddened leaf veins. These symptoms were observed in a field in northeast Louisiana during the summer of 2019 approximately 2 weeks after cotton aphid infestation. Grafting infected cotton plants with healthy ones resulted in similar symptom development, and molecular diagnosis initially indicated and then confirmed the presence of CLRDV in sampled and grafted specimens, respectively. This the first report of CLRDV in Louisiana.

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Cotton blue disease in central-west Brazil: Occurrence, vector (Aphis gossypii) control levels and cultivar reaction

Tropical Plant Pathology ◽

10.1007/s40858-017-0165-1 ◽

2017 ◽

Vol 42 (6) ◽

pp. 468-474

Author(s):

Rafael Galbieri ◽

Alberto S. Boldt ◽

Leonardo B. Scoz ◽

Sandra M. Rodrigues ◽

Diego O. Rabel ◽

...

Keyword(s):

Aphis Gossypii ◽

Cotton Blue ◽

Cotton Blue Disease

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First Report of Cotton Leafroll Dwarf Virus in Cotton Plants Affected by Cotton Leafroll Dwarf Disease in North Carolina

Plant Disease ◽

10.1094/pdis-02-20-0335-pdn ◽

2020 ◽

Vol 104 (12) ◽

pp. 3275

Author(s):

Lindsey D. Thiessen ◽

Tyler Schappe ◽

Marcio Zaccaron ◽

Kassie Conner ◽

Jenny Koebernick ◽

...

Keyword(s):

North Carolina ◽

Dwarf Virus ◽

First Report ◽

Cotton Plants ◽

Dwarf Disease ◽

Cotton Leafroll Dwarf Virus

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Cotton Leafroll Dwarf Virus US Genomes Comprise Divergent Subpopulations and Harbor Extensive Variability

Viruses ◽

10.3390/v13112230 ◽

2021 ◽

Vol 13 (11) ◽

pp. 2230

Author(s):

Roberto Ramos-Sobrinho ◽

Raphael O. Adegbola ◽

Kathy Lawrence ◽

Drew W. Schrimsher ◽

Thomas Isakeit ◽

...

Keyword(s):

South America ◽

Purifying Selection ◽

The United States ◽

Dwarf Virus ◽

Reading Frame ◽

Southern Us ◽

The Us ◽

Cotton Plants ◽

Variable Gene ◽

Cotton Leafroll Dwarf Virus

Cotton leafroll dwarf virus (CLRDV) was first reported in the United States (US) in 2017 from cotton plants in Alabama (AL) and has become widespread in cotton-growing states of the southern US. To investigate the genomic variability among CLRDV isolates in the US, complete genomes of the virus were obtained from infected cotton plants displaying mild to severe symptoms from AL, Florida, and Texas. Eight CLRDV genomes were determined, ranging in size from 5865 to 5867 bp, and shared highest nucleotide identity with other CLRDV isolates in the US, at 95.9–98.7%. Open reading frame (ORF) 0, encoding the P0 silencing suppressor, was the most variable gene, sharing 88.5–99.6% and 81.2–89.3% amino acid similarity with CLRDV isolates reported in cotton growing states in the US and in Argentina and Brazil in South America, respectively. Based on Bayesian analysis, the complete CLRDV genomes from cotton in the US formed a monophyletic group comprising three relatively divergent sister clades, whereas CLRDV genotypes from South America clustered as closely related sister-groups, separate from US isolates, patterns reminiscent of phylogeographical structuring. The CLRDV isolates exhibited a complex pattern of recombination, with most breakpoints evident in ORFs 2 and 3, and ORF5. Despite extensive nucleotide diversity among all available CLRDV genomes, purifying selection (dN/dS < 1) was implicated as the primary selective force acting on viral protein evolution.

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