scholarly journals Phylogeography and Molecular Species Delimitation of Pratylenchus capsici n. sp., a New Root-Lesion Nematode in Israel on Pepper (Capsicum annuum)

2019 ◽  
Vol 109 (5) ◽  
pp. 847-858 ◽  
Author(s):  
Xue Qing ◽  
Wim Bert ◽  
Abraham Gamliel ◽  
Patricia Bucki ◽  
Svetlana Duvrinin ◽  
...  

Root-lesion nematodes of the genus Pratylenchus parasitize the roots of numerous plants and can cause severe damage and yield loss. Here, we report on a new species, Pratylenchus capsici n. sp., from the Arava rift, Israel, which was characterized by integrative methods, including detailed morphology, molecular phylogeny, population genetics, and phylogeography. This species is widely spread across the Arava rift, causing significant infection in pepper (Capsicum annuum) roots and inhibiting plant growth. Both morphological and molecular species delimitation support the recovered species as a new species. We found high cytochrome oxidase subunit I haplotype diversity, and phylogeography analysis suggests that contemporary gene flow is prevented among different agricultural farms, while population dispersal from weeds (Chenopodium album and Sonchus oleraceus) to pepper occurs on a relatively small scale. Our results suggest that weeds are an important reservoir for the dispersal of P. capsici n. sp., either as the original nematode source or at least in maintaining the population between growing seasons.

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Simon Vitecek ◽  
Mladen Kučinić ◽  
Ana Previšić ◽  
Ivana Živić ◽  
Katarina Stojanović ◽  
...  

ZooKeys ◽  
2019 ◽  
Vol 892 ◽  
pp. 93-133 ◽  
Author(s):  
Alejandro Valdez-Mondragón ◽  
Claudia I. Navarro-Rodríguez ◽  
Karen P. Solís-Catalán ◽  
Mayra R. Cortez-Roldán ◽  
Alma R. Juárez-Sánchez

A new species of the spider genus Loxosceles Heineken & Lowe, 1832, Loxosceles tenochtitlan Valdez-Mondragón & Navarro-Rodríguez, sp. nov., is described based on adult male and female specimens from the states of Mexico City, Estado de Mexico and Tlaxcala. Integrative taxonomy including traditional morphology, geometric and lineal morphology, and molecules (DNA barcodes of cytochrome c oxidase subunit 1 (CO1) and internal transcribed spacer 2 (ITS2)), were used as evidence to delimit the new species. Four methods were used for molecular analyses and species delimitation: 1) corrected p-distances under neighbor joining (NJ), 2) automatic barcode gap discovery (ABGD), 3) general mixed yule coalescent model (GMYC), and 4) poisson tree processes (bPTP). All molecular methods, traditional, geometric and lineal morphology were consistent in delimiting and recognizing the new species. Loxosceles tenochtitlansp. nov. is closely related to L. misteca based on molecular data. Although both species are morphologically similar, the average p-distance from CO1 data was 13.8% and 4.2% for ITS2 data. The molecular species delimitation methods recovered well-supported monophyletic clusters for samples of L. tenochtitlansp. nov. from Mexico City + Tlaxcala and for samples of L. misteca from Guerrero. Loxosceles tenochtitlansp. nov. is considered a unique species for three reasons: (1) it can be distinguished by morphological characters (genitalic and somatic); (2) the four different molecular species delimitation methods were congruent to separate both species; and (3) there is variation in leg I length of males between both species, with the males of L. misteca having longer legs than males of L. tenochtitlansp. nov., also morphometrically, the shape of tibiae of the palp between males of both species is different.


2014 ◽  
Vol 13 (4) ◽  
Author(s):  
Abbas Nasehi ◽  
Jugah Bin Kadir ◽  
Farnaz Abed Ashtiani ◽  
Mehdi Nasr-Esfahani ◽  
Mui Yun Wong ◽  
...  

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0190385 ◽  
Author(s):  
Hernán Alvarado-Sizzo ◽  
Alejandro Casas ◽  
Fabiola Parra ◽  
Hilda Julieta Arreola-Nava ◽  
Teresa Terrazas ◽  
...  

Phytotaxa ◽  
2018 ◽  
Vol 350 (1) ◽  
pp. 1
Author(s):  
RUI-HONG WANG ◽  
MAO-QIN XIA ◽  
JIN-BO TAN ◽  
CHUAN CHEN ◽  
XIN-JIE JIN ◽  
...  

A new species, Scrophularia jinii (Scrophulariaceae), from Central China is described and illustrated. This new species was formerly misidentified as S. fargesii, from which it differs in many morphological characters. Moreover, it is distinct with all known Scrophularia species in its unique deeply double serrate leaf margin with 3–7 big teeth on each side. Molecular phylogenetic analysis further supports its species delimitation and suggests a close relationship with several Japanese and North American species.


ZooKeys ◽  
2020 ◽  
Vol 975 ◽  
pp. 111-124
Author(s):  
Linlin Yang ◽  
Yingdang Ren

Pima tristriatasp. nov. is described as new to science based on specimens collected from the Ningxia Hui Autonomous Region, China, and P. boisduvaliella (Guenée, 1845) is also treated here for comparison. DNA barcodes of the two species are provided, together with a neighbor-joining tree for species delimitation. A key to the Holarctic species and a distribution map of the Chinese species are presented.


Phytotaxa ◽  
2016 ◽  
Vol 275 (2) ◽  
pp. 159 ◽  
Author(s):  
XIN-JIE JIN ◽  
YAO CHEN ◽  
JOONGKU LEE ◽  
Zhechen Qi ◽  
LuXian Liu ◽  
...  

A new species, Smilax hirtellicaulis (Smilacaceae), from southwestern China is described and illustrated. The new species differs from all known Smilax species in its unique tepals, i.e., basally connate in male flowers but separate in female flowers. Phylogenetic analysis supports its species delimitation, and suggests a sister position to the clade of Smilax sect. Heterosmilax which have flowers with connate tepals. Their closest phylogenetic relationship and resembled flower morphology may represent an evolutionary transition from separate to united tepals in Smilax. It thus provides us an ideal model for studying the evolution and development of such an innovative floral trait.


ZooKeys ◽  
2019 ◽  
Vol 851 ◽  
pp. 27-69
Author(s):  
Rong Yang ◽  
Zhenzhen Wang ◽  
Yanshuang Zhou ◽  
Zongqing Wang ◽  
Yanli Che

This study examined 504 Rhabdoblatta specimens sampled from China, of which, 86 Rhabdoblatta specimens were used for COI sequencing. A phylogenetic analysis using the ML method and MOTUs estimations by ABGD and GMYC based on COI sequences was performed. Eighteen Rhabdoblatta species were identified when these data were combined with morphological data. Six new species were established among these samples, i.e., Rh.similsinuatasp. n., Rh.densimaculatasp. n., Rh.gyroflexasp. n., Rh.chaulformissp. n., Rh.maculatasp. n., and Rh.ecarinatasp. n. For the first time, females including female genitalia of 14 known Rhabdoblatta species are described worldwide. Our study shows that combining molecular species delimitation methods with morphological data helps to delimit species and understand cockroach biodiversity.


Phytotaxa ◽  
2020 ◽  
Vol 440 (2) ◽  
pp. 89-100
Author(s):  
NABAHAT BESSADAT ◽  
BRUNO HAMON ◽  
NELLY BATAILLE-SIMONEAU ◽  
KIHAL MABROUK ◽  
PHILIPPE SIMONEAU

During a biodiversity survey of Alternaria associated with leaf spot and blight of Solanaceae, a large collection of strains was obtained from samples collected in north-western regions of Algeria in 2013–2018 growing seasons. Amongst these strains, three isolates recovered from tomato and potato had morphological traits different from that usually observed for Alternaria species previously reported on Solanaceae. Based on analysis of a sequence dataset corresponding to portions of the glyceraldehyde-3-phosphate dehydrogenase (gpd), translation elongation factor 1-alpha (tef1) and RNA polymerase second largest subunit (rpb2) genes along with morphological observations, isolates were identified as a new species in the section Japonicae. This novel species, described here as Alternaria telliensis, is phylogenetically and morphologically distinct from A. japonica and A. nepalensis in this section. Pathogenicity tests were performed and isolates were found to be weakly pathogenic to tomato and potato (Solanaceae) while highly aggressive on radish, cabbage and turnip (Brassicaceae) plants.


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