Brown Stem Rot Resistance and Its Modification by Soybean Mosaic Virus in Soybeans

In an effort to identify new sources of resistance to brown stem rot, caused by Phialophora gregata, 829 soybean accessions originating from 14 provinces in central and southern China and ranging in maturity from group IV to group VIII were obtained from the USDA Soybean Germplasm Collection. All accessions were inoculated in sequential evaluations in the greenhouse with three isolates of P. gregata. Putatively resistant accessions were compared statistically with resistant and susceptible standards. Two hundred forty-one, or approximately 29% of the accessions evaluated, were resistant to all three isolates of P. gregata. The percentage of resistant accessions varied among provinces, with the highest percentages identified in the provinces of Anhui, Gansu, Jiangsu, and Sichuan. Resistance to brown stem rot was not associated with maturity of the accessions or presence of soybean mosaic virus. These resistant accessions could be utilized as sources of brown stem rot resistance through integration of northern and southern soybean germ plasm.

Download Full-text

Evaluation of germ plasm for resistant to Soybean mosaic virus (SMV)

JURNAL AGRI-TEK Jurnal Penelitian Ilmu-Ilmu Eksakta ◽

10.33319/agtek.v21i1.66 ◽

2020 ◽

Vol 21 (1) ◽

pp. 6-9

Author(s):

Wuye Ria Andayanie

Keyword(s):

Abiotic Stress ◽

Environmental Factors ◽

Mosaic Virus ◽

Polyclonal Antibody ◽

Symptom Severity ◽

Germ Plasm ◽

Polyclonal Antibodies ◽

Soybean Mosaic Virus ◽

High Yield ◽

High Yields

Soybean superior varieties with high yields and are resistant to abiotic stress have been largely released, although some varieties grown in the field are not resistant to SMV. In addition, the opportunity to obtain lines of hope as prospective varieties with high yield and resistance to SMV is very small. The method for evaluating soybean germplasm is based on serological observations of 98 accessions of leaf samples from SMV inoculation with T isolate. The evaluation results of 98 accessions based on visual observations showed 31 genotypes reacting very resistant or healthy to mild resistant category to SMV T isolate with a percentage of symptom severity of 0 −30 %. Among 31 genotypes there are 2 genotypes (PI 200485; M8Grb 44; Mlg 3288) with the category of visually very resistant and resistant, respectively and Mlg 3288 with the category of mild resistant. They have a good agronomic appearance with a weight of 100 seeds (˃10 g) and react negatively with polyclonal antibodies to SMV, except Mlg 3288 reaction is not consistent, despite the weight of 100 seeds (˃ 10 g). Leaf samples from 98 accessions revealed various symptoms of SMV infection in the field. This diversity of symptoms is caused by susceptibility to accession, when infection occurs, and environmental factors. Keywords—: soybean; genotipe; Soybean mosaic virus (SMV); disease severity; polyclonal antibody

Download Full-text