Viruslike Particles and Double-Stranded RNA inGeotrichum candidum,the Causal Agent of Citrus Sour Rot

1984 ◽  
Vol 74 (8) ◽  
pp. 921 ◽  
Author(s):  
Henia Mor
Keyword(s):  
Causal Agent ◽  
Double Stranded Rna ◽  
Sour Rot ◽  
Viruslike Particles ◽  
Citrus Sour Rot

Antifungal activity of some Moroccan plants against Geotrichum candidum, the causal agent of postharvest citrus sour rot

2012 ◽  
Vol 35 ◽  
pp. 41-46 ◽  
Author(s):  
I. Talibi ◽  
L. Askarne ◽  
H. Boubaker ◽  
E.H. Boudyach ◽  
F. Msanda ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Geotrichum Candidum ◽  
Causal Agent ◽  
Sour Rot ◽  
Citrus Sour Rot

Three different M1 RNA-containing viruslike particle types in Saccharomyces cerevisiae: in vitro M1 double-stranded RNA synthesis

1986 ◽  
Vol 6 (5) ◽  
pp. 1552-1561
Author(s):  
R Esteban ◽  
R B Wickner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rna Synthesis ◽  
Major Coat Protein ◽  
Double Stranded Rna ◽  
Dsrna Molecule ◽  
Protein Toxin ◽  
New Type ◽  
Viruslike Particles ◽  
M1 Rna

Killer strains of Saccharomyces cerevisiae bear at least two different double-stranded RNAs (dsRNAs) encapsidated in 39-nm viruslike particles (VLPs) of which the major coat protein is coded by the larger RNA (L-A dsRNA). The smaller dsRNA (M1 or M2) encodes an extracellular protein toxin (K1 or K2 toxin). Based on their densities on CsCl gradients, L-A- and M1-containing particles can be separated. Using this method, we detected a new type of M1 dsRNA-containing VLP (M1-H VLP, for heavy) that has a higher density than those previously reported (M1-L VLP, for light). M1-H and M1-L VLPs are present together in the same strains and in all those we tested. M1-H, M1-L, and L-A VLPs all have the same types of proteins in the same approximate proportions, but whereas L-A VLPs and M1-L VLPs have one dsRNA molecule per particle, M1-H VLPs contain two M1 dsRNA molecules per particle. Their RNA polymerase produces mainly plus single strands that are all extruded in the case of M1-H particles but are partially retained inside the M1-L particles to be used later for dsRNA synthesis. We show that M1-H VLPs are formed in vitro from the M1-L VLPs. We also show that the peak of M1 dsRNA synthesis is in fractions lighter than M1-L VLPs, presumably those carrying only a single plus M1 strand. We suggest that VLPs carrying two M1 dsRNAs (each 1.8 kilobases) can exist because the particle is designed to carry one L-A dsRNA (4.5 kilobases).

IN VITRO AND IN VIVO ANTIFUNGAL ACTIVITY OF EIGHT MEDICINAL PLANTS AGAINST CITRUS SOUR ROT AGENT GEOTRICHUM CANDIDUM

2015 ◽  
pp. 1563-1570 ◽  
Author(s):  
Idriss Talibi ◽  
Latifa Askarne ◽  
Hassan Boubaker ◽  
El Hassane Boudyach ◽  
Abdellah Ait Ben Oumar
Keyword(s):  
Antifungal Activity ◽  
Medicinal Plants ◽  
Geotrichum Candidum ◽  
Sour Rot ◽  
Citrus Sour Rot ◽  
In Vivo Antifungal Activity

scholarly journals Curative activity of postharvest GRAS salt treatments to control citrus sour rot caused by Geotrichum citri-aurantii

2020 ◽  
Vol 335 ◽  
pp. 108860 ◽  
Author(s):  
Lourdes Soto-Muñoz ◽  
Verònica Taberner ◽  
Beatriz de la Fuente ◽  
Nihed Jerbi ◽  
Lluís Palou
Keyword(s):  
Sour Rot ◽  
Citrus Sour Rot

scholarly journals Antifungal properties of organic extracts of eightCistusL. species against postharvest citrus sour rot

2015 ◽  
Vol 62 (1) ◽  
pp. 16-22 ◽  
Author(s):  
H. Karim ◽  
H. Boubaker ◽  
L. Askarne ◽  
I. Talibi ◽  
F. Msanda ◽  
...  
Keyword(s):  
Sour Rot ◽  
Antifungal Properties ◽  
Organic Extracts ◽  
Citrus Sour Rot

EFFECT OF ORGANIC AND INORGANIC SALTS AS ALTERNATIVE STRATEGY FOR THE CONTROL OF POSTHARVEST CITRUS SOUR ROT AGENT GEOTRICHUM CANDIDUM

2015 ◽  
pp. 1577-1583
Author(s):  
Idriss Talibi ◽  
Latifa Askarne ◽  
Hassan Boubaker ◽  
El Hassane Boudyach ◽  
Abdellah Ait Ben Oumar
Keyword(s):  
Geotrichum Candidum ◽  
Alternative Strategy ◽  
Inorganic Salts ◽  
Sour Rot ◽  
Citrus Sour Rot

scholarly journals K28, a unique double-stranded RNA killer virus of Saccharomyces cerevisiae.

1990 ◽  
Vol 10 (9) ◽  
pp. 4807-4815 ◽  
Author(s):  
M J Schmitt ◽  
D J Tipper
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytoplasmic Membrane ◽  
Yeast Cells ◽  
Cross Hybridization ◽  
Double Stranded Rna ◽  
Killer System ◽  
Dsrna Viruses ◽  
Viruslike Particles ◽  
Killer Virus

The double-stranded RNA (dsRNA) viruses of Saccharomyces cerevisiae consist of 4.5-kilobase-pair (kb) L species and 1.7- to 2.1-kb M species, both found in cytoplasmic viruslike particles (VLPs). The L species encode their own capsid protein, and one (LA) has been shown to encode a putative capsid-polymerase fusion protein (cap-pol) that presumably provides VLPs with their transcriptase and replicase functions. The M1 and M2 dsRNAs encode the K1 and K2 toxins and specific immunity mechanisms. Maintenance of M1 and M2 is dependent on the presence of LA, which provides capsid and cap-pol for M dsRNA maintenance. Although a number of different S. cerevisiae killers have been described, only K1 and K2 have been studied in any detail. Their secreted polypeptide toxins disrupt cytoplasmic membrane functions in sensitive yeast cells. K28, named for the wine S. cerevisiae strain 28, appears to be unique; its toxin is unusually stable and disrupts DNA synthesis in sensitive cells. We have now demonstrated that 4.5-kb L28 and 2.1-kb M28 dsRNAs can be isolated from strain 28 in typical VLPs, that these VLPs are sufficient to confer K28 toxin and immunity phenotypes on transfected spheroplasts, and that the immunity of the transfectants is distinct from that of either M1 or M2. In vitro transcripts from the M28 VLPs show no cross-hybridization to denatured M1 or M2 dsRNAs, while L28 is an LA species competent for maintenance of M1. K28, encoded by M28, is thus the third unique killer system in S. cerevisiae to be clearly defined. It is now amenable to genetic analysis in standard laboratory strains.

scholarly journals Hypovirulence-Associated Double-Stranded RNA from Sclerotinia homoeocarpa Is Conspecific with Ophiostoma novo-ulmi Mitovirus 3a-Ld

2003 ◽  
Vol 93 (11) ◽  
pp. 1407-1414 ◽  
Author(s):  
F. Deng ◽  
R. Xu ◽  
G. J. Boland
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Horizontal Transmission ◽  
Phytopathogenic Fungi ◽  
Causal Agent ◽  
Dsrna Virus ◽  
Dutch Elm Disease ◽  
Sclerotinia Homoeocarpa ◽  
Double Stranded Rna ◽  
Reading Frame

The nucleotide sequence of the hypovirulence-associated double-stranded RNA (dsRNA) in hypovirulent isolate Sh12B of Sclerotinia homoeocarpa, the causal agent of dollar spot of turf grass, was determined. This large dsRNA (L-dsRNA) is 2,632 bp long and is A and U rich (61.0% A+U residues). One strand of this dsRNA contains an open reading frame (ORF) with the potential to encode a protein of 720 amino acids. This ORF contains 12 UGA codons, predicted to encode tryptophan in ascomycete mitochondria, and has a codon bias typical of mitochondrial genes, which is consistent with a mitochondrial localization of this dsRNA. The amino acid sequence contains conserved motifs typical of RNA-dependent RNA polymerases (RdRps). Sequence analyses of the nucleotide and RdRp-like protein revealed that the L-dsRNA is homologous with previously characterized mitochondrial viruses and dsRNAs from other phytopathogenic fungi, and shares 92.4% nucleotide and 95.1% amino acid sequence identities with the Ophiostoma novo-ulmi mitovirus 3a-Ld from Ophiostoma novo-ulmi, the causal agent of Dutch elm disease. The results indicate that these two dsRNAs are conspecific. This is the first report that a hypovirulence-associated dsRNA virus naturally occurs in two taxonomically distinct fungi, and indicates that horizontal transmission of this dsRNA virus may have occurred between these fungi.

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