scholarly journals Evidence of Genetic Exchange by Parasexual Recombination and Genetic Analysis of Pathogenicity and Mating Type of Parasexual Recombinants in Rice Blast Fungus, Magnaporthe oryzae

2006 ◽  
Vol 96 (7) ◽  
pp. 746-750 ◽  
Author(s):  
M. T. Noguchi ◽  
N. Yasuda ◽  
Y. Fujita
Keyword(s):  
Mating Type ◽  
Magnaporthe Oryzae ◽  
Genomic Dna ◽  
Rice Blast ◽  
Southern Blot Analysis ◽  
Selectable Marker ◽  
Marker Gene ◽  
Rice Blast Fungus ◽  
Selectable Marker Gene ◽  
Sexual Cross

A selectable marker gene conferring resistance to bialaphos (BI) was introduced into rice blast isolate Y90-71BI and another conferring resistance to blasticidin S (BS) into isolate 3514-R-2BS of Magnaporthe oryzae to demonstrate exchange of DNA. Colonies obtained from co-cultures of these two isolates were resistant to both BI and BS and had both resistance genes as shown by Southern blot analysis of their genomic DNA. Conidia from these BI-BS-resistant isolates had only one nucleus per cell after staining with 4′,6-diamidino-2-phenylindole (DAPI). Using flow cytometry, however, these BI-BS-resistant isolates were found to be haploid. Segregation of BI-BS-resistant isolates for pathogenicity (avirulence to virulence) on rice line K59-1 was consistent with a 1:1 ratio, as was segregation for mating type. These BI-BS-resistant isolates were thus apparently derived from parasexual exchange of DNA and the segregation of pathogenicity and of mating type of the parasexual recombinants might correspond to that of the progeny of the offspring of the sexual cross.

scholarly journals Distribution of Mating Type and Sexual Status in Chinese Rice Blast Populations

Plant Disease ◽  
2009 ◽  
Vol 93 (3) ◽  
pp. 238-242 ◽  
Author(s):  
Jing Zeng ◽  
Shujie Feng ◽  
Jiangqiao Cai ◽  
Ling Wang ◽  
Fei Lin ◽  
...  
Keyword(s):  
Mating Type ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
The Other ◽  
Fertility Level ◽  
Mating Types ◽  
Field Isolates ◽  
Sexual Status ◽  
Blast Fungus

A collection of 520 field isolates of the rice blast fungus (Magnaporthe oryzae) originating from five provinces in China was assessed for mating type and sexual fertility. One of the two tester sets was composed of isolates collected from barley and the other from rice. Two mating types (MAT1-1 and MAT1-2) were identified among the 443 fertile isolates. The two mating types were roughly in balance with one another in the southwestern region but one or the other predominated in the southeastern and southern regions. Male-only fertile isolates were the most common, and only a few hermaphroditic and no female only fertile isolates were detected. The fertility level of the isolates was variable. Isolates from Jiangsu were more fertile than those from Fujian. The mating capacity of the testers collected from barley was higher than that of those collected from rice, but this was because the MAT1-2 testers differed very significantly from one another. In contrast, the mating capacities of the two MAT1-1 testers were similar to one another.

scholarly journals Investigating the cell and developmental biology of plant infection by the rice blast fungus Magnaporthe oryzae

2021 ◽  
pp. 103562
Author(s):  
Alice Bisola Eseola ◽  
Lauren S. Ryder ◽  
Míriam Osés-Ruiz ◽  
Kim Findlay ◽  
Xia Yan ◽  
...  
Keyword(s):  
Developmental Biology ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
Plant Infection ◽  
Blast Fungus

scholarly journals Tanzawaic acids I–L: Four new polyketides from Penicillium sp. IBWF104-06

2014 ◽  
Vol 10 ◽  
pp. 251-258 ◽  
Author(s):  
Louis P Sandjo ◽  
Eckhard Thines ◽  
Till Opatz ◽  
Anja Schüffler
Keyword(s):  
Soil Sample ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
Fungal Strain ◽  
Conidial Germination ◽  
Penicillium Sp ◽  
Culture Filtrates ◽  
Blast Fungus ◽  
New Compounds

Four new polyketides have been identified in culture filtrates of the fungal strain Penicillium sp. IBWF104-06 isolated from a soil sample. They are structurally based on the same trans-decalinpentanoic acid skeleton as tanzawaic acids A–H. One of the new compounds was found to inhibit the conidial germination in the rice blast fungus Magnaporthe oryzae at concentrations of 25 μg/mL.

A plant selectable marker gene based on the detoxification of the herbicide dalapon

1992 ◽  
Vol 11 (12) ◽  
Author(s):  
Vicky Buchanan-Wollaston ◽  
Andrea Snape ◽  
Frank Cannon
Keyword(s):  
Selectable Marker ◽  
Marker Gene ◽  
Selectable Marker Gene

scholarly journals Septin-Mediated Plant Cell Invasion by the Rice Blast Fungus, Magnaporthe oryzae

Science ◽  
2012 ◽  
Vol 336 (6088) ◽  
pp. 1590-1595 ◽  
Author(s):  
Y. F. Dagdas ◽  
K. Yoshino ◽  
G. Dagdas ◽  
L. S. Ryder ◽  
E. Bielska ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Invasion ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
Blast Fungus

scholarly journals Lessons in effector and NLR biology of plant-microbe systems

2017 ◽  
Author(s):  
Aleksandra Białas ◽  
Erin K. Zess ◽  
Juan Carlos De la Concepcion ◽  
Marina Franceschetti ◽  
Helen G. Pennington ◽  
...  
Keyword(s):  
Plant Physiology ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Molecular Mechanisms ◽  
Rice Blast Fungus ◽  
Leucine Rich Repeat ◽  
Nucleotide Binding Domain ◽  
Immune Receptors ◽  
Microbe Interactions ◽  
Host Infection

A diversity of plant-associated organisms secrete effectors—proteins and metabolites that modulate plant physiology to favor host infection and colonization. However, effectors can also activate plant immune receptors, notably nucleotide-binding domain and leucine-rich repeat-containing (NLR) proteins, enabling plants to fight off invading organisms. This interplay between effectors, their host targets, and the matching immune receptors is shaped by intricate molecular mechanisms and exceptionally dynamic coevolution. In this article, we focus on three effectors, AVR-Pik, AVR-Pia, and AVR-Pii, from the rice blast fungus Magnaporthe oryzae (syn. Pyricularia oryzae), and their corresponding rice NLR immune receptors, Pik, Pia, and Pii, to highlight general concepts of plant-microbe interactions. We draw 12 lessons in effector and NLR biology that have emerged from studying these three little effectors and are broadly applicable to other plant-microbe systems.

scholarly journals Genome-wide polyadenylation site mapping datasets in the rice blast fungus Magnaporthe oryzae

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Marco Marconi ◽  
Ane Sesma ◽  
Julio Luis Rodríguez-Romero ◽  
María Lourdes Rosano González ◽  
Mark D. Wilkinson
Keyword(s):  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
Polyadenylation Site ◽  
Blast Fungus ◽  
Site Mapping ◽  
Genome Wide

scholarly journals Manipulating genome of diploid potato inbred line Solanumchacoense M6 using selectable marker gene

2020 ◽  
Vol 44 (4) ◽  
pp. 399-407
Author(s):  
Sarbesh Das DANGOL ◽  
İlknur YEL ◽  
Mehmet Emin ÇALIŞKAN ◽  
Allah BAKHSH
Keyword(s):  
Inbred Line ◽  
Selectable Marker ◽  
Marker Gene ◽  
Diploid Potato ◽  
Selectable Marker Gene
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