scholarly journals Genetic Diversity of Pathogenic and Nonpathogenic Populations of Fusarium oxysporum Isolated from Carnation Fields in Argentina

2004 ◽  
Vol 94 (6) ◽  
pp. 661-668 ◽  
Author(s):  
Gladys Lori ◽  
Véronique Edel-Hermann ◽  
Nadine Gautheron ◽  
Claude Alabouvette
Keyword(s):  
Genetic Diversity ◽  
Fusarium Oxysporum ◽  
Intergenic Spacer ◽  
Vegetative Compatibility ◽  
Genetic Homogeneity ◽  
Vegetative Compatibility Group ◽  
Local Populations ◽  
Compatibility Group ◽  
Pathogenicity Tests ◽  
Reference Strains

In order to elucidate the origin of Fusarium oxysporum f. sp. dianthi in Argentina, the genetic diversity among pathogenic isolates together with co-occurring nonpathogenic isolates on carnation was investigated. In all, 151 isolates of F. oxysporum were obtained from soils and carnation plants from several horticultural farms in Argentina. The isolates were characterized using vegetative compatibility group (VCG), intergenic spacer (IGS) typing, and pathogenicity tests on carnation. Seven reference strains of F. oxysporum f. sp. dianthi also were analyzed and assigned to six different IGS types and six VCGs. Twenty-two Argentinean isolates were pathogenic on carnation, had the same IGS type (50), and belonged to a single VCG (0021). The 129 remaining isolates were nonpathogenic on carnation and sorted into 23 IGS types and 97 VCGs. The same VCG never occurred in different IGS types. Our results suggest that the pathogen did not originate in the local populations of F. oxysporum but, rather, that it was introduced into Argentina. Given the genetic homogeneity within Argentinean isolates of F. oxysporum f. sp. dianthi, either IGS type or VCG can be used for the identification of the forma specialis dianthi currently in Argentina.

Further investigations of vegetative compatibility within Fusarium oxysporum f.sp. melonis

1990 ◽  
Vol 68 (6) ◽  
pp. 1245-1248 ◽  
Author(s):  
D. J. Jacobson ◽  
T. R. Gordon
Keyword(s):  
Genetic Diversity ◽  
North America ◽  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Group ◽  
Culture Collections ◽  
Vegetative Compatibility Groups ◽  
The Third ◽  
Geographic Origins ◽  
Compatibility Group

One hundred and nineteen strains of Fusarium oxysporum f.sp. melonis were characterized by virulence and vegetative compatibility. One hundred and seven strains were placed in four previously reported vegetative compatibility groups: 0130, 0131, 0133, and 0134. Four strains were placed in three new vegetative compatibility groups, and the remaining eight strains were vegetatively self-incompatible. Two of the three new vegetative compatibility groups shared similar geographic origins and distribution with two previously reported vegetative compatibility groups; the third represented a more isolated infestation. All vegetatively self-incompatible isolates originated from culture collections; none have been recently isolated from nature. These newly characterized strains extend our knowledge of genetic diversity in F. oxysporum f.sp. melonis. All four F. oxysporum f.sp. melonis races exist in more than one vegetative compatibility group. European strains represent four vegetative compatibility groups, one of which is present in North America and another in the Middle East. The significance of this diversity is unknown, as are the phylogenetic relationships among strains in this forma specialis.

Fusarium oxysporum associated with wilt and root rot of tomato in Queensland; races and vegetative compatibility groups

1992 ◽  
Vol 32 (5) ◽  
pp. 651 ◽  
Author(s):  
MD Ramsey ◽  
RG O'Brien ◽  
KG Pegg
Keyword(s):  
Fusarium Oxysporum ◽  
Root Rot ◽  
Vascular Tissue ◽  
Group Analysis ◽  
Vegetative Compatibility ◽  
Crown Rot ◽  
Vegetative Compatibility Group ◽  
Vegetative Compatibility Groups ◽  
Compatibility Group ◽  
Pathogenicity Tests

Twenty-two isolates of Fusarium oxysporum, from Queensland's major tomato growing areas, were studied in glasshouse pathogenicity tests and assessed for vegetative compatibility. Isolates of Fusarium oxysporum f. sp. lycopersici were identified to race using pathogenicity tests with 4 differential tomato cultivars: Grosse Lisse, Scorpio, moradade and Delta Tristar. The occurrence of race 3 in the Bundaberg district in 1988 was established. In glasshouse experiments, Fusarium wilt severity was influenced by inoculum concentration (1 x 106 v. 5 x 106 conidia/ml). Pathogenic and non-pathogenic isolates were distinguished by vegetative compatibility group analysis. However, all races were in a single vegetative compatibility group and could not be differentiated using this technique. Isolates collected from discoloured vascular tissue in the lower stems of plants with severe root rot (Pythium spp. associated), were non-pathogenic to tomato, bean and pea, although some isolates caused slight damage to cucumber. These isolates were distinctly different from Fusarium oxysporum f. sp. radicis-lycopersici, the cause of root and crown rot.

scholarly journals Characterization of Fusarium oxysporum Isolates Obtained from Cucumber in China by Pathogenicity, VCG, and RAPD

Plant Disease ◽  
2004 ◽  
Vol 88 (6) ◽  
pp. 645-649 ◽  
Author(s):  
D. J. Vakalounakis ◽  
Z. Wang ◽  
G. A. Fragkiadakis ◽  
G. N. Skaracis ◽  
D.-B. Li
Keyword(s):  
Fusarium Oxysporum ◽  
Random Amplified Polymorphic Dna ◽  
Vegetative Compatibility ◽  
Stem Rot ◽  
Chinese Isolate ◽  
Vegetative Compatibility Group ◽  
Group I ◽  
Compatibility Group ◽  
First Time

Thirty-four isolates of Fusarium oxysporum, obtained in China from cucumber plants showing either Fusarium wilt (F. oxysporum f. sp. cucumerinum) or root and stem rot (F. oxysporum f. sp. radicis-cucumerinum) symptoms, were characterized by pathogenicity, vegetative compatibility, and random amplified polymorphic DNA (RAPD). Of these, 23 isolates were identified by pathogenicity as F. oxysporum f. sp. cucumerinum, and one as F. oxysporum f. sp. radicis-cucumerinum, while 10 isolates were avirulent on cucumber, melon, sponge gourd, and pumpkin. The Chinese isolates of F. oxysporum f. sp. cucumerinum were assigned to RAPD groups III and XXI and to vegetative compatibility group (VCG) 0183, four new VCGs, 0184 to 0187, and a single-member VCG included in the artificial VCG 018-. The Chinese isolate of F. oxysporum f. sp. radicis-cucumerinum was assigned to RAPD group I and bridging VCG 0260/0261. The occurrence of F. oxysporum f. sp. radicis-cucumerinum on cucumber is reported for the first time in China.

scholarly journals Vegetative Compatibility Groups of Fusarium oxysporum f. sp. lactucae from Lettuce

Plant Disease ◽  
2005 ◽  
Vol 89 (3) ◽  
pp. 237-240 ◽  
Author(s):  
Matias Pasquali ◽  
Flavia Dematheis ◽  
Giovanna Gilardi ◽  
Maria Lodovica Gullino ◽  
Angelo Garibaldi
Keyword(s):  
Fusarium Oxysporum ◽  
Genetic Relatedness ◽  
The United States ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Group ◽  
Vegetative Compatibility Groups ◽  
Compatibility Group ◽  
Race 1

Fusarium oxysporum f. sp. lactucae, the causal agent of Fusarium wilt of lettuce, has been reported in three continents in the last 10 years. Forty-seven isolates obtained from infected plants and seed in Italy, the United States, Japan, and Taiwan were evaluated for pathogenicity and vegetative compatibility. Chlorate-resistant, nitrate-nonutilizing mutants were used to determine genetic relatedness among isolates from different locations. Using the vegetative compatibility group (VCG) approach, all Italian and American isolates, type 2 Taiwanese isolates, and a Japanese race 1 were assigned to the major VCG 0300. Taiwanese isolates type 1 were assigned to VCG 0301. The hypothesis that propagules of Fusarium oxysporum f. sp. lactucae that caused epidemics on lettuce in 2001-02 in Italian fields might have spread via import and use of contaminated seeds is discussed.

Characterisation of Strains of Fusarium oxysporum f.sp. cubense by Production of Volatiles

1991 ◽  
Vol 39 (2) ◽  
pp. 161 ◽  
Author(s):  
NY Moore ◽  
PA Hargreaves ◽  
KG Pegg ◽  
JAG Irwin
Keyword(s):  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Group ◽  
Compatibility Group ◽  
Race 1 ◽  
Race 2 ◽  
Race 4

The production of volatiles on steamed rice by Australian isolates of Fusarium oxysporum f. sp. cubense correlated well with race and vegetative compatibility group (VCG). All race 4 isolates (VCGs 0120, 0129) produced distinctive volatile odours which gave characteristic gas chromatograms where the num- ber of peaks equated to VCG. Race 1 (VCGs 0124, 0125) and race 2 (VCG 0128) isolates, as well as non-pathogenic isolates of F. oxysporum from the banana rhizosphere, did not produce detectable volatiles and gave chromatograms without significant peaks.

scholarly journals Characterization of a Regional Population of Fusarium oxysporum f. sp. niveum by Race, Cross Pathogenicity, and Vegetative Compatibility

2007 ◽  
Vol 97 (4) ◽  
pp. 461-469 ◽  
Author(s):  
X. G. Zhou ◽  
K. L. Everts
Keyword(s):  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Infested Soil ◽  
Vegetative Compatibility Group ◽  
Atlantic Region ◽  
Compatibility Group ◽  
Race 1 ◽  
Regional Population ◽  
Race 2

Eighty-eight isolates of Fusarium oxysporum f. sp. niveum, collected from wilted watermelon plants and infested soil in Maryland and Dela-ware, were characterized by cross pathogenicity to muskmelon, race, and vegetative compatibility. Four isolates (4.5%) were moderately pathogenic to ≥2 of 18 muskmelon cultivars in a greenhouse test, and one representative isolate also was slightly pathogenic in field microplots. The four isolates all were designated as race 2, and were in vegetative compatibility group (VCG) 0082. Of the 74 isolates to which a VCG could be assigned, 41 were in VCG 0080, the VCG distributed most widely; 27 were in VCG 0082, and were distributed in half of the 20 watermelon fields surveyed; and 6 were in the newly described VCG 0083, and were restricted to three fields. Among the isolates in VCG 0080, 8 were designated as race 0, 21 as race 1, and 12 as race 2. Of the isolates in VCG 0082, 6 were designated as race 0, 11 as race 1, and 10 as race 2. All isolates in VCG 0083 were designated as race 2. Isolates from more than one race within the same VCG or isolates from more than one VCG were recovered from single plants and fields. No differences in aggressiveness on differential watermelon cultivars were observed among isolates from different VCGs of the same race. A diverse association between virulence and VCG throughout the Mid-Atlantic region suggests that the pathotypes of F. oxysporum f. sp. niveum may be of local origin or at least long existent in the region.

scholarly journals Race Determination and Vegetative Compatibility Grouping of Fusarium oxysporum f. sp. melonis from South Africa

Plant Disease ◽  
2000 ◽  
Vol 84 (3) ◽  
pp. 231-234 ◽  
Author(s):  
W. Schreuder ◽  
S. C. Lamprecht ◽  
G. Holz
Keyword(s):  
South Africa ◽  
Fusarium Oxysporum ◽  
South African ◽  
Geographic Region ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Group ◽  
Compatibility Group ◽  
Race 1 ◽  
Race 2 ◽  
High Degree

Isolates of Fusarium oxysporum f. sp. melonis (72 total) obtained from 30 fields in 17 melonproducing regions in South Africa were race typed, using differential cvs. CM 17187, Doublon, Perlita, and Topmark, and grouped on the basis of vegetative compatibility. Fifty-four isolates were identified as race 0, eight as race 1, and ten as race 2. Race 0 occurred in 15 of 17 regions, whereas race 1 was sporadically recovered. Race 2 was obtained from only four fields located in one geographic region. Perlita plants (carrying the gene Fom3) inoculated with local isolates of races 0 and 2 and reference isolates of race 0 became stunted, and their leaves became yellow, thickened, and brittle. Using two inoculation methods, similar symptoms were induced by reference and local isolates of race 0 on Perlita seedlings. The results indicated that Fom3 in Perlita confers a tolerant reaction compared with the resistant reaction of gene Fom1 in Doublon and, therefore, should not be used alone in race determination tests. All isolates belonged to vegetative compatibility group 0134, indicating a high degree of genetic homogeneity among the South African F. oxysporum f. sp. melonis population.

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