scholarly journals Role of Cumulus Cells and Serum on the In Vitro Maturation, Fertilization, and Subsequent Development of Rat Oocytes1

1989 ◽  
Vol 40 (4) ◽  
pp. 720-728 ◽  
Author(s):  
B. C. Vanderhyden ◽  
D. T. Armstrong
Keyword(s):  
In Vitro Maturation ◽  
Subsequent Development ◽  
Cumulus Cells

In vitro maturation and glutathione synthesis of porcine oocytes in the presence or absence of cysteamine under different oxygen tensions: role of cumulus cells

2002 ◽  
Vol 14 (3) ◽  
pp. 125 ◽  
Author(s):  
Y. Z. Bing ◽  
Y. Hirao ◽  
K. Iga ◽  
L. M. Che ◽  
N. Takenouchi ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
In Vitro Maturation ◽  
Cumulus Cells ◽  
Vitro Fertilization ◽  
Male Pronucleus ◽  
Nuclear Maturation ◽  
Maturation Medium ◽  
Oxygen Tensions

The present study was conducted to evaluate the effect of cumulus cells on the in vitro maturation (IVM) and glutathione (GSH) synthesis of porcine oocytes cultured in the presence or absence of cysteamine under different oxygen tensions, and on their subsequent male pronucleus formation after in vitro fertilization (IVF). Cumulus-oocyte complexes (COCs) and cumulus-denuded oocytes (DOs) were cultured for 45 h in modified TCM-199 supplemented with or without 150 m cysteamine under a humidified atmosphere of 5% CO2 in air (20%�O2) or 5% CO2, 5% O2 and 90% N2. When cultured in medium supplemented with cysteamine under 20% O2 tension, the rates of COC maturation to the metaphase II (MII) stage were significantly higher than those of DOs (P<0.05). Regardless of the addition of cysteamine and oxygen tension, the rates of male pronucleus formation in COCs after IVM and IVF were significantly higher than in DOs (P<0.05). The GSH content of oocytes was significantly increased by the addition of cysteamine to the maturation medium (P<0.05), with significantly higher GSH content in COCs than in DOs (P<0.05). However, the GSH content of COCs and DOs was not significantly different when cultured in medium without cysteamine. These results indicate that cumulus cells play an important role in nuclear maturation to MII, GSH synthesis in porcine oocytes cultured in the presence of cysteamine, and subsequent male pronucleus formation after IVF.

scholarly journals Peroxiredoxin 6 Is Upregulated in Bovine Oocytes and Cumulus Cells During In Vitro Maturation: Role of Intercellular Communication1

2004 ◽  
Vol 71 (5) ◽  
pp. 1646-1651 ◽  
Author(s):  
Gregory Leyens ◽  
Benjamin Verhaeghe ◽  
Marie Landtmeters ◽  
Joëlle Marchandise ◽  
Bernard Knoops ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Cumulus Cells ◽  
Peroxiredoxin 6 ◽  
Bovine Oocytes

scholarly journals Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?

2020 ◽  
pp. 1
Author(s):  
Tulay Irez ◽  
Sinem Ercan Dogan ◽  
Enver Ciraci ◽  
Saadet Busra Aksoyer ◽  
Muhammet Sait Toprak ◽  
...  
Keyword(s):  
Experimental Study ◽  
Luteinizing Hormone ◽  
Embryo Development ◽  
In Vitro Maturation ◽  
Germinal Vesicle ◽  
Cumulus Cells ◽  
Immature Oocytes ◽  
Maturation Rate

<p><strong>OBJECTIVE:</strong> In this study, we aimed to investigate the role of the cumulus cell’s apoptosis parameter in the maturation of immature rescue oocytes. </p><p><strong>STUDY DESIGN:</strong> In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matured germinal vesicle oocytes were compared with apoptotic properties of cumulus cells. </p><p><strong>RESULTS:</strong> In all of the immature oocytes after oocyte in vitro maturation, the maturation rate has been observed as 56.1% and 2PN rate as 63.0%. Afterin vitro maturation of germinal vesicle oocytes, there was no difference in apoptosis rates of the cumulus cells between mature and immature oocytes (p&gt; 0.05). The ratio of 2PN in matured germinal vesicle oocytes showing embryo development was 35.4%. A positive correlation was found between luteinizing hormone values on day 3 and E2 values during HCG days during oocyte maturation and embryo development (p=0.021, p=0.020). In addition, it has been observed that the germinal vesicle oocytes, which have completed their maturation and developed into embryos, have high E2 values during HCG days (p=0.020).</p><p><strong>CONCLUSION:</strong> In our study, it has been demonstrated that in vitro maturation in rescue oocytes from stimulated cycles, embryo development potential could not be explained by the apoptosis parameter.</p>

Role of Cumulus Cells on In Vitro Maturation of Canine Oocytes

2007 ◽  
Vol 42 (2) ◽  
pp. 184-189 ◽  
Author(s):  
T Otoi ◽  
T Shin ◽  
DC Kraemer ◽  
ME Westhusin
Keyword(s):  
In Vitro Maturation ◽  
Cumulus Cells

scholarly journals In-vitro maturation of human germinal vesicle stage oocytes: role of cumulus cells and epidermal growth factor in the culture medium

1998 ◽  
Vol 13 (6) ◽  
pp. 1638-1644 ◽  
Author(s):  
P. T. Goud ◽  
A. P. Goud ◽  
C. Qian ◽  
H. Laverge ◽  
J. Van der Elst ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
In Vitro Maturation ◽  
Culture Medium ◽  
Germinal Vesicle ◽  
Cumulus Cells ◽  
Epidermal Growth

55 The Role of Poly (ADP-Ribose) Polymerases in Porcine Cumulus - Oocyte Complex During In Vitro Maturation and Embryonic Development

2018 ◽  
Vol 30 (1) ◽  
pp. 166
Author(s):  
D. H. Kim ◽  
S. T. Shin ◽  
H. T. Lee
Keyword(s):  
Embryonic Development ◽  
In Vitro Maturation ◽  
Parp Inhibitor ◽  
Development Program ◽  
Cumulus Cells ◽  
Treated Group ◽  
Blastocyst Stage ◽  
Mrna Levels

Poly(ADP-ribosyl)ation (PARylation) is related to DNA repair, chromatin modification, and apoptosis and is catalyzed by PARylation polymerases (PARP). Previous studies have shown that PARylation regulates pre-implantation development and participates autophagy mechanism in mouse and pig. However, the involvement of PARylation and pro-survival autophagy in pre-implantation development from cumulus–oocyte complexes (COC) to the blastocyst stage has not yet been documented. Thus, we investigated the role of PARylation during in vitro maturation (IVM) of porcine COC and their embryonic development. To study the effect of PARylation, COC were cultured with 3-aminobenzamide (3-ABA, PARP inhibitor) during IVM. Nuclear maturation rates of oocytes were showed no significant differences between 2 groups in all stages (from GV to MII). However, the expansion rates of cumulus cells were significantly decreased in 3-ABA–treated COC compared with control (11.05 ± 1.09 v. 48.40 ± 0.67%). When we analysed mRNA levels of maturation- and expansion-related genes in cumulus cells, levels of PTX3, CX43, and COX-2 were increased but levels of HAS2 and TNFAIP6 were decreased in treatment group. In addition, expression levels of GDF9 and BMP15 in oocytes were up-regulated in treated group. Then, we examined the development of IVF embryos from IVM oocytes in the presence and absence of 3-ABA and their quality at the blastocyst stage. We found that the developmental rates of embryos were significantly decreased in 3-ABA-treated group. In particular, the proportion of expanded blastocysts was lower in the treated embryos (2.65 ± 1.53) compared with control embryos (12.27 ± 3.05). Furthermore, the transcript levels of autophagy-related genes (ATG5, BECLIN1, and LC3) in 3-ABA-treated embryos were lowered in all stages. In addition, we found a higher rate of apoptosis in treated blastocysts compared with the control (total apoptosis index; 15.65 ± 2.73 v. 4.89 ± 0.67). Finally, SQSTM1/p62 aggregate increased in 3-ABA-treated blastocysts, indicating that the inhibition of PARylation regulates selective autophagy pathways to utilise SQSTM1/p62. Therefore, these results indicate that PARylation by PARPs during IVM of COC is deeply involved in the pro-survival autophagy and influences the development and quality of porcine embryos. This research was supported by a Grant from the Bio & Medical Technology Development Program (2015M3A9C7030091) of the National Research Foundation (NRF) funded by the Korean government.

In vitro culture of embryos produced by in vitro maturation and fertilization of bovine follicular oocytes

1989 ◽  
Vol 1989 ◽  
pp. 14-14
Author(s):  
V. Vergos ◽  
A. Gordon ◽  
M. Gallagher ◽  
I. Gordon
Keyword(s):  
In Vitro Maturation ◽  
Present Report ◽  
Subsequent Development ◽  
Cumulus Cells ◽  
Blastocyst Stage ◽  
Fertilization In Vitro ◽  
Factors Affecting ◽  
Stage Of Development

A previous report from this laboratory dealt with the establishment of pregnancies in the early months of gestation after the non-surgical transfer of cattle embryos derived from the in vitro maturation (IVM) of primary bovine oocytes, their fertilization in vitro (IVF) and their subsequent development to the transferable stage (morula/blastocyst) using an in vivo (sheep oviduct) culture system (Lu et al.,1987). The present report deals with some factors affecting the efficiency of IVF and with the culture in vitro of zygotes to the morula/ blastocyst stage of development. Some embryos were frozen and after thawing transferred by non-surgical procedures to five recipient cattle to obtain information on their capacity to undergo further embryonic development.Primary oocytes, enclosed in cumulus cells, were recovered from vesicular follicles (2-6mm) after their dissection from the ovaries of heifers slaughtered at a local abattoir. The ovaries were brought to the laboratory within one hour of animal slaughter in medium held at 35'C.

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