scholarly journals Morphological and Molecular Analysis of Pinnipedia(Otariodae, Odobenidae, Phocidae); with Classification of New Fossil Material

2021 ◽  
Vol 35 (S1) ◽  
Author(s):  
Azizah Hafed ◽  
Sulman Rahmat ◽  
Irina Koretsky
Keyword(s):  
Molecular Analysis ◽  
Fossil Material

A revised infrageneric classification of Bellevalia Lapeyr. (Asparagaceae: Scilloideae) based on molecular analysis

Phytotaxa ◽  
2021 ◽  
Vol 525 (1) ◽  
pp. 70-84
Author(s):  
AZARNOOSH JAFARI ◽  
JAMIL VAEZI ◽  
MOHAMMAD MAHDI FORGHANIFARD ◽  
FÉLIX FOREST ◽  
JOHN C. MANNING
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Analysis ◽  
Trnl Intron ◽  
Leaf Margin ◽  
Infrageneric Classification ◽  
Flower Shape ◽  
Representative Species

Within the genus Bellevalia (Asparagaceae: Scilloideae: Hyacintheae), flower shape and colour, the ratio of leaf to scape length, and the orientation of the pedicels were traditionally used to delimit sections, subsections and species. We conducted a phylogenetic analysis of representative species from sections Bellevalia, Conicae, Nutantes and Oxydontae, representing four out of the six sections that are currently recognised, using the four chloroplast regions rbcL, matK, trnL intron, and trnL-F spacer. Our results indicate that the sections are not monophyletic as currently circumscribed. Our analyses retrieve two major, well-supported clades. The first clade (sect. Conicae) includes only species with ciliate leaves and green-veined perianth lobes, and the second clade (sect. Bellevalia) includes the species lacking these characteristics. Within the second clade, the species with yellow anthers are separated from those with violet anthers. Our molecular analysis does not support the traditional subdivisions of the genus but establishes the value of cilia on the leaf margin, green veins in the perianths lobes, and the colour of the anthers as indicators of relationships among the species. We recommend that the current four sections and six subsections of Bellevalia be reduced to two sections without subsections and we provide an updated and corrected nomenclature for these sections and subsections, designating lectotypes where necessary.

scholarly journals MPC-09 THE OPTIMIZATION OF TREATMENTS FOR SO-CALLED PRIMITIVE NEUROECTODERMAL TUMORS WITH MOLECULAR ANALYSIS

2019 ◽  
Vol 1 (Supplement_2) ◽  
pp. ii23-ii24
Author(s):  
Mario Suzuki ◽  
Naohide Fujita ◽  
Ikuko Ogino ◽  
Junya Fujimura ◽  
Akihide Kondo
Keyword(s):  
Molecular Analysis ◽  
High Grade Glioma ◽  
Pediatric Brain Tumors ◽  
High Throughput Analysis ◽  
Institutional Review ◽  
Neuroectodermal Tumors ◽  
Molecular Information ◽  
Pediatric Brain ◽  
Informed Parental Consent

Abstract INTRODUCTION In the previous WHO classification of central nervous tumors, the supratentorial tumors comprise small round blue cells with aggressive clinical features had been defined as primitive neuroectodermal tumor (PNET). Recent molecular analysis revealed that they do not belong to a single entity, but they are re-classified as the tumors of other well-defined tumors and newly defined tumor species. These facts were reflected to the new classifications. While, there are few studies those showed the re-consideration of treatments for tumors diagnosed as so-called PNET. In this study, we propose the optimization of treatments for tumors diagnosed by the new classification to clarify which treatments were effective for the tumors those were diagnosed as PNET. MATERIALS AND METHODS The tumor samples diagnosed as so-called PNETs were analyzed. The molecular information was extracted from tumor specimens. We used high throughput analysis with microarray, FISH, and immunohistochemistry. They all had treated in our institution in last 6 years and their clinical courses were followed by medical records. Informed parental consent was obtained from their guardians and this study was approved by the institutional review board of Juntendo university. RESULTS Nine tumor samples were able to be analyzed and they are re-classified into high-grade glioma, neuroblastoma, sarcoma, embryonal tumors with multilayered rosettes, C19MC altered (ETMR). They resembled each other closely in morphology, and therefore, it was not able to be classified by histopathological findings. There was a case of pineoblastoma, whose molecular background suggested that the tumor was re-classified into neuroblastoma. In terms of treatments, we have succeeded in neuroblastoma cases so far, ETMRs were required multiple surgeries and radiations to maintain remissions. CONCLUSIONS Re-classification of diagnosis based on the molecular background is necessary to clarify the optimization of treatments for pediatric brain tumors, and the comprehensive methods is required. We present our methods for molecular diagnosis in clinical field and future plans.

scholarly journals Molecular analysis and biochemical classification of TDP-43 proteinopathy

Brain ◽  
2012 ◽  
Vol 135 (11) ◽  
pp. 3380-3391 ◽  
Author(s):  
Hiroshi Tsuji ◽  
Tetsuaki Arai ◽  
Fuyuki Kametani ◽  
Takashi Nonaka ◽  
Makiko Yamashita ◽  
...  
Keyword(s):  
Molecular Analysis

A new genus for North American Cicadetta species (Hemiptera: Cicadidae)

Zootaxa ◽  
2017 ◽  
Vol 4306 (4) ◽  
pp. 537
Author(s):  
DAVID C. MARSHALL ◽  
KATHY B. R. HILL
Keyword(s):  
Molecular Analysis ◽  
North American ◽  
Type Species ◽  
New Genus ◽  
Morphological Characters ◽  
Globally Distributed

The generic classification of cicadas within the globally distributed tribe Cicadettini (Hemiptera: Cicadidae) has been challenging due to their often conservative morphology. A recent molecular analysis has indicated that the six North American taxa currently classified in Cicadetta are unrelated to the European type species of Cicadetta, C. montana Scopoli. Here we identify a set of diagnostic morphological characters for a new genus, which we distinguish from its closest relatives in Eurasia and Australasia. 

Central European parasitic flatworms of the Cyclocoelidae Stossich, 1902 (Trematoda: Plagiorchiida): molecular and comparative morphological analysis suggests the reclassification of Cyclocoelum obscurum (Leidy, 1887) into the Harrahium Witenberg, 1926

Parasitology ◽  
2016 ◽  
Vol 144 (4) ◽  
pp. 368-383 ◽  
Author(s):  
JILJÍ SITKO ◽  
JIŘÍ BIZOS ◽  
PETR HENEBERG
Keyword(s):  
Molecular Analysis ◽  
Molecular Phylogenetics ◽  
Morphological Analysis ◽  
Central European ◽  
Diagnostic Characters ◽  
Comparative Morphological Analysis ◽  
The Family ◽  
Single Clade

SUMMARYCyclocoelidae Stossich, 1902 are medium-sized to large digenean bird parasites. Although these parasites bear few visible autapomorphic signs, and their diagnostic characters are unstable in response to the pressure applied during preparation, the numerous hitherto suggested re-classifications within the family have not been supported by any molecular analysis. We analyse here cyclocoelids found during the extensive examination of central European birds performed from 1962 to 2016, provide comparative measurements, host spectra, prevalence and intensity, and provide and analyse sequences of four DNA loci of five of the cyclocoelid species. Cyclocoleum Brandes, 1892 appears paraphyletic; thus we suggest the re-classification of Cyclocoleum obscurum (Leidy, 1887) as Harrahium obscurum (Leidy, 1887) Sitko and Heneberg comb. n. Molecular phylogenetics questioned also the validity of Cyclocoelinae Stossich, 1902 and Hyptiasminae Dollfus, 1948, which formed a single clade, whereas Allopyge Johnston, 1913, Prohyptiasmus Witenberg, 1923 and Morishitium Witenberg, 1928 formed another clade. Haematotrephinae Dollfus, 1948 are newly characterized as having a pretesticular or intertesticular ovary that forms a triangle with the testes. Analyses of non-European genera of the Cyclocoelidae and an examination of the position of families within Echinostomata La Rue, 1926 are needed.

scholarly journals Phylogeny and higher classification of Mutillidae (Hymenoptera) based on morphological reanalyses

2017 ◽  
Vol 60 ◽  
pp. 1-97 ◽  
Author(s):  
Denis J. Brothers ◽  
Arkady S. Lelej
Keyword(s):  
Molecular Analysis ◽  
Morphological Characters ◽  
Broad Agreement ◽  
Cladistic Analyses

This study aimed to resolve the differences in the two currently used classifications of Mutillidae, which differ in many respects. Cladistic analyses of 101 genera and subgenera of Mutillidae (represented by females of 253 species and males of 260 species) and four outgroups (pepsine Pompilidae, anthoboscine Tiphiidae and both fedtschenkiine and sapygine Sapygidae) based on 230 morphological characters treated in various ways, produced most-parsimonious trees which were in broad agreement but differed in many details. Evaluation of these results led to the proposal of a compromise tree which reflected each proposed taxon as monophyletic, while trying to keep disruptions to the current classifications to a minimum. The result differs from both previous classifications, and proposes the recognition of eight subfamilies: Myrmosinae (with the tribes Kudakrumiini and Myrmosini), Pseudophotopsidinae, Rhopalomutillinae, Ticoplinae (with the tribes Smicromyrmillini and Ticoplini), Sphaeropthalminae (with the tribes Sphaeropthalmini, Dasymutillinitrib. n., and Pseudomethocini with the subtribes Euspinoliinasubtrib. n.and Pseudomethocina), Myrmillinae, Dasylabrinae (with the tribes Apteromutillinitrib. n.and Dasylabrini) and Mutillinae (with the tribes Ctenotillinitrib. n., Smicromyrmini, Mutillini with the subtribes Ephutina and Mutillina, and Trogaspidiini). Notably, Myrmosinae were consistently strongly supported as monophyletic with the remaining Mutillidae (disagreeing with a recent molecular analysis), and thus retained as a mutillid subfamily. The placements of all currently valid genera and subgenera in the proposed classification are provided.

scholarly journals Mucopolysaccharidosis type VI (MPS VI) and molecular analysis: Review and classification of published variants in theARSBgene

2018 ◽  
Vol 39 (12) ◽  
pp. 1788-1802 ◽  
Author(s):  
Rosella Tomanin ◽  
Litsa Karageorgos ◽  
Alessandra Zanetti ◽  
Moeenaldeen Al-Sayed ◽  
Mitch Bailey ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Mucopolysaccharidosis Type ◽  
Mucopolysaccharidosis Type Vi ◽  
Mps Vi

scholarly journals Molecular typing of Polish strains of Babesia canis protozoa isolated from dogs in the years 2013-2016

2018 ◽  
Vol 74 (1) ◽  
pp. 6021-2018
Author(s):  
PAWEŁ ŁYP ◽  
MACIEJ SKRZYPCZAK ◽  
STANISŁAW WINIARCZYK ◽  
ŁUKASZ ADASZEK
Keyword(s):  
Gene Polymorphism ◽  
Molecular Analysis ◽  
Molecular Typing ◽  
Clinical Disease ◽  
Babesia Canis ◽  
Restriction Digestion ◽  
Gene Fragment ◽  
Pcr Products ◽  
Rna Genes

The aim of the study was the molecular analysis of B. canis strains isolated from dogs from Poland based on 18S RNA and Bc28 genes. The study involved 140 protozoan strains derived from clinical disease cases. All DNA samples of parasites were analyzed in two ways (amplification and restriction digestion) which made it possible to demonstrate the polymorphism of the Bc28 gene, and to show 18S RNA gene polymorphism (amplification and restriction digestion). Amplification of the Bc28 gene fragment and digestion of the resulting PCR products allowed for the classification of 104 isolates of B. canis to the Bc28-A group, and 36 strains of protozoa to the Bc28-B group. Amplification of the Bc28 gene fragment and digestion of the resulting PCR products allowed for the classification of 33 isolates to 18S RNA-A group, while to 18S RNA-B – 107 parasite isolates. Comparison of both groups of protozoa among themselves allowed partial but not complete correlation of polymorphisms in Bc28 and 18S RNA genes..

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