scholarly journals Chlorogenic Acid Relaxes Rat Ileal Smooth Muscles by Mechanisms Independent of Rho Kinase Mediated Effects on Myosin Light Chain Phosphorylation

2021 ◽  
Vol 35 (S1) ◽  
Author(s):  
Erin Ashley ◽  
John Nybo ◽  
Zachary Wieland ◽  
Ekua Amelemah ◽  
Elisha Injeti
Keyword(s):  
Chlorogenic Acid ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Smooth Muscles ◽  
Rho Kinase ◽  
Myosin Light Chain Phosphorylation ◽  
Mediated Effects

Isoproterenol attenuates myosin phosphorylation and contraction of tracheal muscle

1989 ◽  
Vol 66 (5) ◽  
pp. 2017-2022 ◽  
Author(s):  
K. Obara ◽  
P. de Lanerolle
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Isometric Force ◽  
Smooth Muscles ◽  
Inhibitory Effect ◽  
Isometric Tension ◽  
Myosin Light Chain Phosphorylation ◽  
Response Curves ◽  
Shortening Velocity ◽  
Myosin Phosphorylation

The effects of isoproterenol on isometric force, unloaded shortening velocity, and myosin phosphorylation were examined in thin muscle bundles (0.1–0.2 mm diam) dissected from lamb tracheal smooth muscle. Methacholine (10(-6) M) induced rapid increases in isometric force and in phosphorylation of the 20,000-Da myosin light chain. Myosin phosphorylation remained elevated during steady-state maintenance of isometric force. The shortening velocity peaked at 15 s after stimulation with methacholine and then declined to approximately 45% of the maximal value by 3 min. Isoproterenol pretreatment inhibited methacholine-stimulated myosin light chain phosphorylation, shortening velocity, and force during the early stages of force generation. However, the inhibitory effect of isoproterenol on force and myosin phosphorylation is proportionally greater than that on shortening velocity. Isoproterenol pretreatment also caused a rightward non-parallel shift in the methacholine dose-response curves for both isometric tension and myosin light chain phosphorylation. These data demonstrate that isoproterenol attenuates the contractile properties of airway smooth muscles by affecting the rate and extent of myosin light chain phosphorylation, perhaps through a mechanism that involves the synergistic interaction of myosin light chain kinase phosphorylation and Ca2+ metabolism.

Increased Ca2+ and myosin phosphorylation, but not calmodulin activity in sensitized airway smooth muscles

1995 ◽  
Vol 269 (1) ◽  
pp. L135-L135
Author(s):  
He Jiang ◽  
Kang Rao ◽  
Xueliang Liu ◽  
Gang Liu ◽  
Newman L. Stephans
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Smooth Muscles ◽  
Ragweed Pollen ◽  
Myosin Light Chain Phosphorylation ◽  
Myosin Phosphorylation

Page L739: He Jiang, Kang Rao, Xueliang Liu, Gang Liu, and Newman L. Stephans. “Increased Ca2+ and myosin phosphorylation, but not calmodulin activity in sensitized airway smooth muscles.” This title is incorrect and conveys the opposite meaning of the article. The correct title should read: “Intracellular Ca2+, myosin light chain phosphorylation, and calmodulin activity in ragweed pollen-sensitized airway smooth muscles.”

Effects of Rho-kinase inhibition on myosin light chain phosphorylation and obstruction-induced detrusor overactivity

2013 ◽  
Vol 21 (3) ◽  
pp. 319-324 ◽  
Author(s):  
James O Marx ◽  
Maureen E Basha ◽  
Sunish Mohanan ◽  
Joseph A Hypolite ◽  
Shaohua Chang ◽  
...  
Keyword(s):  
Light Chain ◽  
Detrusor Overactivity ◽  
Myosin Light Chain ◽  
Rho Kinase ◽  
Myosin Light Chain Phosphorylation ◽  
Kinase Inhibition

Rho–Rho kinase is involved in smooth muscle cell migration through myosin light chain phosphorylation-dependent and independent pathways

2001 ◽  
Vol 155 (2) ◽  
pp. 321-327 ◽  
Author(s):  
Shingo Ai ◽  
Masafumi Kuzuya ◽  
Teruhiko Koike ◽  
Toshinobu Asai ◽  
Shigeru Kanda ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cell Migration ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Rho Kinase ◽  
Myosin Light Chain Phosphorylation ◽  
Smooth Muscle Cell Migration

scholarly journals Cytoplasmic free calcium, myosin light chain phosphorylation, and force in phasic and tonic smooth muscle.

1988 ◽  
Vol 92 (6) ◽  
pp. 713-729 ◽  
Author(s):  
B Himpens ◽  
G Matthijs ◽  
A V Somlyo ◽  
T M Butler ◽  
A P Somlyo
Keyword(s):  
Smooth Muscle ◽  
Pulmonary Artery ◽  
Light Chain ◽  
Time Course ◽  
Myosin Light Chain ◽  
Muscle Tension ◽  
Smooth Muscles ◽  
Free Calcium ◽  
Myosin Light Chain Phosphorylation ◽  
Tonic Smooth Muscle

The time course of [Ca2+]i, tension, and myosin light chain phosphorylation were determined during prolonged depolarization with high K+ in intact tonic (rabbit pulmonary artery) and phasic (longitudinal layer of guinea pig ileum) smooth muscles. [Ca2+]i was monitored with the 340 nm/380 nm signal ratio of the fluorescent indicator fura-2. The fluorescence ratio had a similar time course in both muscle types during depolarization with 109 mM [K+]o; after a transient peak, there was a decline to 70% of its peak value in tonic smooth muscle, and to 60% in phasic smooth muscle. Tension, however, continued to increase in the pulmonary artery, while in the ileum it declined in parallel with the [Ca2+]i. On changing [K+]o from 109 to 20 mM, tension and [Ca2+]i either remained unchanged or declined in parallel in the pulmonary artery. Phosphorylation of the 20-kD myosin light chain, measured during stimulation of muscle strips with 109 mM [K+]o in another set of experiments, increased from 3% to a peak of 50% in the intact pulmonary artery, and then declined to a steady state value of 23%. In the intact ileum, a very rapid, early transient phosphorylation (up to 50%) at 2-3 s was seen. This transient declined by 30 s to a value that was close to the resting level (7%), while tension remained at 55% of its peak force. A quick release during maintained stimulation induced no detectable change in the [Ca2+]i in either type of smooth muscle. We discuss the possibility that the slowly rising tonic tension in pulmonary artery could be due to cooperativity between phosphorylated and nonphosphorylated crossbridges.

scholarly journals Dynamic Regulation of Myosin Light Chain Phosphorylation by Rho-kinase

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e39269 ◽  
Author(s):  
Takako Kaneko-Kawano ◽  
Fugo Takasu ◽  
Honda Naoki ◽  
Yuichi Sakumura ◽  
Shin Ishii ◽  
...  
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Rho Kinase ◽  
Myosin Light Chain Phosphorylation ◽  
Dynamic Regulation
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