scholarly journals Arachidonic acid (AA) and Prostaglandin E2‐induced cerebral vasodilation is mediated by Carbon Monoxide independent of Reactive Oxygen Species in the newborn pig

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Alie Joseph Kanu ◽  
Charles W. Leffler
Keyword(s):  
Reactive Oxygen Species ◽  
Carbon Monoxide ◽  
Arachidonic Acid ◽  
Prostaglandin E2 ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Cerebral Vasodilation

scholarly journals Arachidonic acid- and prostaglandin E2-induced cerebral vasodilation is mediated by carbon monoxide, independent of reactive oxygen species in piglets

2011 ◽  
Vol 301 (6) ◽  
pp. H2482-H2487 ◽  
Author(s):  
Alie Kanu ◽  
Charles W. Leffler
Keyword(s):  
Reactive Oxygen Species ◽  
Carbon Monoxide ◽  
Arachidonic Acid ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Artificial Cerebrospinal Fluid ◽  
Newborn Pigs ◽  
Cerebral Vasodilation ◽  
Prostacyclin Analog ◽  
Cerebral Vascular

Arachidonic acid (AA) and prostaglandin (PG) E2 stimulate carbon monoxide (CO) production, and AA metabolism is known to be associated with the generation of reactive oxygen species (ROS). This study was conducted to address the hypothesis that CO and/or ROS mediate cerebrovascular dilation in newborn pigs. Experiments were performed on anesthetized newborn pigs with closed cranial windows. Different concentrations of AA (10−8-10−6 M), PGE2 (10−8-10−6 M), iloprost (10−8-10−6 M), and their vehicle (artificial cerebrospinal fluid) were given. Piglets with PGE2 and iloprost received indomethacin (5 mg/kg iv) to inhibit cyclooxygenase. AA, PGE2, and iloprost caused concentration-dependent increases in pial arteriolar diameter. The effects of both AA and PGE2 in producing cerebral vascular dilation and associated CO production were blocked by the heme oxygenase inhibitor chromium mesoporphyrin (2 × 10−5 M), but not by the prostacyclin analog, iloprost. ROS inhibitor tempol (SOD mimetic) (1 × 10−5 M) and the H2O2 scavenger catalase (1,000 U/ml) also do not block these vasodilator effects of AA and PGE2. Heme-l-lysinate-induced cerebrovascular dilation and CO production was blocked by chromium mesoporphyrin. Hypoxanthine plus xanthine oxidase, a combination that is known to generate ROS, caused pial arteriolar dilation and CO production that was inhibited by tempol and catalase. These data suggest that AA- and PGE2-induced cerebral vascular dilation is mediated by CO, independent of ROS.

scholarly journals Role of reactive oxygen species in LPS-induced production of prostaglandin E2 in microglia

2004 ◽  
Vol 88 (4) ◽  
pp. 939-947 ◽  
Author(s):  
Tongguang Wang ◽  
Liya Qin ◽  
Bin Liu ◽  
Yuxin Liu ◽  
Belinda Wilson ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Prostaglandin E2 ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Regulation by reactive oxygen species of interleukin-1β, nitric oxide and prostaglandin E2 production by human chondrocytes

2002 ◽  
Vol 10 (7) ◽  
pp. 547-555 ◽  
Author(s):  
M. Mathy-Hartert ◽  
G.P. Deby-Dupont ◽  
J.-Y.L. Reginster ◽  
N. Ayache ◽  
J.-P. Pujol ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Prostaglandin E2 ◽  
Reactive Oxygen ◽  
Interleukin 1Β ◽  
Human Chondrocytes ◽  
Oxygen Species
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