scholarly journals The role of I614 and E615 in unnatural substrate recognition in Taq DNA polymerase mutants (967.5)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Andrea Gochi ◽  
Hayley Schultz ◽  
Aaron Leconte
Keyword(s):  
Dna Polymerase ◽  
Substrate Recognition ◽  
Taq Dna Polymerase ◽  
Polymerase Mutants

scholarly journals Efforts towards crystallization of Taq DNA polymerase mutants (967.4)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Alfredo Valencia ◽  
Jacqueline Kroll ◽  
Matthew Sazinsky ◽  
Aaron Leconte
Keyword(s):  
Dna Polymerase ◽  
Taq Dna Polymerase ◽  
Polymerase Mutants

Taq DNA Polymerase Mutants and 2′-Modified Sugar Recognition

Biochemistry ◽  
2015 ◽  
Vol 54 (38) ◽  
pp. 5999-6008 ◽  
Author(s):  
Hayley J. Schultz ◽  
Andrea M. Gochi ◽  
Hannah E. Chia ◽  
Alexie L. Ogonowsky ◽  
Sharon Chiang ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Taq Dna Polymerase ◽  
Polymerase Mutants ◽  
Sugar Recognition

scholarly journals Comparative characterization of Taq DNA polymerase mutants capable of recognizing 2' modified nucleotides (967.3)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Aaron Leconte ◽  
Hannah Chia ◽  
Emma Hadley ◽  
Andrea Gochi ◽  
Alfredo Valencia
Keyword(s):  
Dna Polymerase ◽  
Modified Nucleotides ◽  
Taq Dna Polymerase ◽  
Comparative Characterization ◽  
Polymerase Mutants

scholarly journals The SOS Error-Prone DNA Polymerase V Mutasome and β-Sliding Clamp Acting in Concert on Undamaged DNA and during Translesion Synthesis

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1083
Author(s):  
Adhirath Sikand ◽  
Malgorzata Jaszczur ◽  
Linda B. Bloom ◽  
Roger Woodgate ◽  
Michael M. Cox ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Pathogenic Bacteria ◽  
Fold Increase ◽  
Translesion Dna Synthesis ◽  
Sliding Clamp ◽  
Pol V ◽  
Dna Polymerase V ◽  
Acting In Concert

In the mid 1970s, Miroslav Radman and Evelyn Witkin proposed that Escherichia coli must encode a specialized error-prone DNA polymerase (pol) to account for the 100-fold increase in mutations accompanying induction of the SOS regulon. By the late 1980s, genetic studies showed that SOS mutagenesis required the presence of two “UV mutagenesis” genes, umuC and umuD, along with recA. Guided by the genetics, decades of biochemical studies have defined the predicted error-prone DNA polymerase as an activated complex of these three gene products, assembled as a mutasome, pol V Mut = UmuD’2C-RecA-ATP. Here, we explore the role of the β-sliding processivity clamp on the efficiency of pol V Mut-catalyzed DNA synthesis on undamaged DNA and during translesion DNA synthesis (TLS). Primer elongation efficiencies and TLS were strongly enhanced in the presence of β. The results suggest that β may have two stabilizing roles: its canonical role in tethering the pol at a primer-3’-terminus, and a possible second role in inhibiting pol V Mut’s ATPase to reduce the rate of mutasome-DNA dissociation. The identification of umuC, umuD, and recA homologs in numerous strains of pathogenic bacteria and plasmids will ensure the long and productive continuation of the genetic and biochemical journey initiated by Radman and Witkin.

The Role of Cytochrome 2B1 Substrate Recognition Site Residues 115, 294, 297, 298, and 362 in the Oxidation of Steroids and 7-Alkoxycoumarins

2001 ◽  
Vol 394 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Tammy L. Domanski ◽  
You-Qun He ◽  
Emily E. Scott ◽  
Qinmi Wang ◽  
James R. Halpert
Keyword(s):  
Substrate Recognition ◽  
Recognition Site
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